Diagenode

Ty1 Antibody – ChIP Grade

目录号
格式
价格
C15200054
(MAb-054-050)
50 µg/29 µl
$380.00
  Bulk order
其他格式



Monoclonal antibody raised in mouse against the Ty1 tag (amino acid sequence EVHTNQDPLD).

Lot007
Concentration2.2 µg/µl
Species reactivity
TypeMonoclonal
PurityProtein A purified monoclonal antibody in PBS containing 0.05% azide.
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2 μg/ChIP Fig 1
Western Blotting 1:1,000 - 1:2,000 Fig 2, 3

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data
    Ty1 Antibody validated in ChIP

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody against the Ty1 tag
    ChIP assays were performed using Plasmodium falciparium cells stably expressing Ty1-H2A (blue bars) and wild type Plasmodium falciparium cells (brown bars). Primer pairs were used for active genes, inactive genes, and variable genes. Sheared chromatin from 1x10e6 cells and 2 μg of antibody against Ty1 (cat. No. C15200054) were used per ChIP experiment

    Ty1 Antibody validated in WB

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the Ty1 tag
    Western blot was performed on 40 µg whole cell extracts from HeLa cells transfected with Ty1-tagged MBD1. The WB was performed with the Diagenode antibody against the Ty1 tag (cat. No. C15200054) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk (lane 2) or with an MBD1 antibody (C15410078, lane 1). The position of the Ty1-tagged MBD1 and Ty1-tagged MBD2 are shown on the right, the marker (in kDa) is shown on the left.

    Ty1 Antibody validated in WB

    Figure 3. Western blot analysis using the Diagenode monoclonal antibody against the Ty1 tag
    Western blot was performed on 40 μg whole cell extracts from HeLa cells transfected with either Ty1-tagged MBD1 (lanes 1 and 3) or Ty1-tagged MBD2 (lane 2 and 4). The WB was performed with the Diagenode antibody against the Ty1 tag (Cat. No. C15200054) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (lane 1 and 2) or with an MBD1 antibody (C15410078, lane 3) or an MBD2 antibody (C15410312, lane 4). The position of the Ty1-tagged MBD1 and Ty1-tagged MBD2 are shown on the right, the marker (in kDa) is shown on the left.

  •  应用
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  文档
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Datasheet Ty1 C15200054 DATASHEET
    Monoclonal antibody raised in mouse against the Ty1 tag (amino acid sequence EVHTNQDPLD).
    Download
  •  Safety sheets
    Ty1 antibody SDS GB en Download
    Ty1 antibody SDS US en Download
    Ty1 antibody SDS BE nl Download
    Ty1 antibody SDS FR fr Download
    Ty1 antibody SDS BE fr Download
    Ty1 antibody SDS ES es Download
    Ty1 antibody SDS DE de Download
    Ty1 antibody SDS JP ja Download
  •  出版物

    How to properly cite this product in your work

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    Using our products in your publication? Let us know!

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