EN | | | / Search

Products
Shearing technologies
Automation
- IP-Star accessories
Chromatin studies
DNA methylation
Genome editing (CRISPR/Cas9)
- Selection of guide RNA by ChIP
Antibodies
NGS Library preparation
Exosomes
Reagents
- Primer indexes for tagmented libraries
Services
Epigenomics Profiling Services
Epigenomics Data Analysis Service
Research areas
Resources
- Protocols
- Publications
- Documents
- Testimonials
- Media pack
- Videos
- Webinars
Company
- About us
- Events
- News
- Careers
- Scientific programs
Contact
- Sales and Technical Support
- How to find us?

Auto Premium Bisulfite kit

Catalog Number

Format

Price

C02030031

40 rxns

$240.00

Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.

Characteristics
Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.

The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 μg, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA
Documents
Auto Premium Bisulfite kit MANUAL
Fastest method for complete bisulfite conversion of DNA for methylation analysis. ... Download

Related products
- C03010010
  
  Auto IPure kit v2
- C02010034
  
  Auto hMeDIP kit x16 (monoclonal mouse antibody)
- C02020011
  
  Auto MethylCap kit
- C02030032
  
  Premium RRBS kit (24 rxns) - replaced by Premiu...
- C02030033
  
  Premium RRBS kit (96 rxns) - replaced by Premiu...

Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'Auto Premium Bisulfite kit',
	'meta_title' => 'Auto Premium Bisulfite kit',
	'product' => array(
		'Product' => array(
			'id' => '1893',
			'antibody_id' => null,
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => 'Characteristics',
			'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '40 rxns',
			'catalog_number' => 'C02030031',
			'old_catalog_number' => '',
			'sf_code' => 'C02030031-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '255',
			'price_USD' => '240',
			'price_GBP' => '230',
			'price_JPY' => '39945',
			'price_CNY' => '',
			'price_AUD' => '600',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-premium-bisulfite-kit-40-rxns',
			'meta_title' => 'Auto Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Auto Premium Bisulfite kit',
			'modified' => '2019-12-16 10:05:26',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'Auto Premium Bisulfite kit'
$meta_title = 'Auto Premium Bisulfite kit'
$product = array(
	'Product' => array(
		'id' => '1893',
		'antibody_id' => null,
		'name' => 'Auto Premium Bisulfite kit',
		'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
		'label1' => 'Characteristics',
		'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '40 rxns',
		'catalog_number' => 'C02030031',
		'old_catalog_number' => '',
		'sf_code' => 'C02030031-',
		'type' => 'REF',
		'search_order' => '04-undefined',
		'price_EUR' => '255',
		'price_USD' => '240',
		'price_GBP' => '230',
		'price_JPY' => '39945',
		'price_CNY' => '',
		'price_AUD' => '600',
		'country' => 'ALL',
		'except_countries' => 'Japan',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'auto-premium-bisulfite-kit-40-rxns',
		'meta_title' => 'Auto Premium Bisulfite kit',
		'meta_keywords' => '',
		'meta_description' => 'Auto Premium Bisulfite kit',
		'modified' => '2019-12-16 10:05:26',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1899',
			'antibody_id' => null,
			'name' => 'Auto IPure kit v2',
			'description' => '<p><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></p>
<p>The DNA recovery after ChIP or MeDIP might be an issue for specific downstream applications (e.g. Next generation sequencing). Diagenode's IPure kit is the only DNA purification kit that is specifically optimized for extracting very low amounts of DNA after ChIP &amp; MeDIP.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 rxns',
			'catalog_number' => 'C03010010',
			'old_catalog_number' => 'AL-Auto01-0100',
			'sf_code' => 'C03010010-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '380',
			'price_USD' => '410',
			'price_GBP' => '340',
			'price_JPY' => '59525',
			'price_CNY' => '',
			'price_AUD' => '1025',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-ipure-kit-v2-x100-100-rxns',
			'meta_title' => 'Auto IPure kit v2 x100',
			'meta_keywords' => '',
			'meta_description' => 'Auto IPure kit v2 x100',
			'modified' => '2019-02-27 15:19:42',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1885',
			'antibody_id' => null,
			'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented&nbsp;genomic DNA&nbsp;samples for use in genome-wide methylation analysis. It features</span><span>&nbsp;a highly specific monoclonal antibody against&nbsp;</span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes&nbsp;control DNA and primers to assess the effiency of the assay.&nbsp;</span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast,&nbsp;reliable and&nbsp;highly specific.</span></p>',
			'label1' => ' Characteristics',
			'info1' => '<ul style="list-style-type: disc;">
<li><span>Robust enrichment &amp; immunoprecipitation of hydroxymethylated DNA</span></li>
<li>Highly specific monoclonal antibody against 5-hmC<span>&nbsp;for reliable, reproducible results</span></li>
<li>Including&nbsp;control DNA and primers to&nbsp;<span>monitor the&nbsp;efficiency of the assay</span>
<ul style="list-style-type: circle;">
<li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li>
<li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li>
</ul>
</li>
</ul>
<ul style="list-style-type: disc;">
<li>Improved single-tube, magnetic bead-based protocol</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '16 rxns',
			'catalog_number' => 'C02010034',
			'old_catalog_number' => '',
			'sf_code' => 'C02010034-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '630',
			'price_USD' => '690',
			'price_GBP' => '580',
			'price_JPY' => '98690',
			'price_CNY' => '',
			'price_AUD' => '1725',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns',
			'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'meta_keywords' => '',
			'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'modified' => '2021-01-18 10:37:19',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1888',
			'antibody_id' => null,
			'name' => 'Auto MethylCap kit',
			'description' => '<p>The Auto MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. This procedure has been optimized to perform automated immunoprecipitation of chromatin using the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a> enabling highly reproducible results and allowing for high throughput.</p>',
			'label1' => ' Characteristics',
			'info1' => '<ul>
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>Automation compatibility</strong><strong></strong>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>F</strong><strong>igure 1.</strong><span>&nbsp;</span>Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).<br /><strong></strong></p>
</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020011',
			'old_catalog_number' => 'AF-Auto01-0048',
			'sf_code' => 'C02020011-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-methylcap-kit-x48-48-rxns',
			'meta_title' => 'Auto MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'Auto MethylCap kit x48',
			'modified' => '2020-09-17 13:33:12',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1894',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (24 rxns) - replaced by Premium RRBS kit v2 C02030036',
			'description' => '<div class="extra-spaced"><center></center>The kit Premium RRBS (24 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a><span>.</span>&nbsp;</div>
<div class="extra-spaced">For the information about the format 96 rxns - check out <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-x96-96-rxns">Premium RRBS (96 rxns)</a>.&nbsp;</div>
<div class="extra-spaced"></div>
<p style="text-align: justify;"></p>',
			'label1' => '',
			'info1' => '',
			'label2' => 'Nature Methods',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>',
			'label3' => '',
			'info3' => '',
			'format' => '24 rxns',
			'catalog_number' => 'C02030032',
			'old_catalog_number' => '',
			'sf_code' => 'C02030032-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '1560',
			'price_USD' => '1640',
			'price_GBP' => '1445',
			'price_JPY' => '272000',
			'price_CNY' => '',
			'price_AUD' => '4100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x24-24-rxns',
			'meta_title' => 'Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit x24 | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium RRBS kit analyzes DNA methylation at the single nucleotide level for 4 million CpGs.Reduced Representation Bisulfite Sequencing is fast and affordable.',
			'modified' => '2022-06-02 14:31:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1895',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
			'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'NATURE METHODS',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '96 rxns',
			'catalog_number' => 'C02030033',
			'old_catalog_number' => '',
			'sf_code' => 'C02030033-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '4415',
			'price_USD' => '4385',
			'price_GBP' => '4085',
			'price_JPY' => '769000',
			'price_CNY' => '',
			'price_AUD' => '10965',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x96-96-rxns',
			'meta_title' => 'Premium RRBS Kit | Diagenode ',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries to efficiently analyze DNA methylation at the single nucleotide level. More cost effective than Whole Genome Bisulfite Sequencing (WGBS) and with greater coverage than microarray-based methods.',
			'modified' => '2022-12-05 11:22:21',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '5',
			'position' => '10',
			'parent_id' => '1',
			'name' => 'Bisulfite conversion',
			'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">Bisulfite modification of DNA is the most commonly used, "<strong>gold standard</strong>" method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. T<span style="font-weight: 400;">his technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.</span></div>
<div style="text-align: justify;" class="large-12 columns"></div>
<div style="text-align: justify;" class="large-12 columns">Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.
<h3>How it works</h3>
Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).
<p class="text-center"><img src="https://www.diagenode.com/img/applications/bisulfite.png" /><br />Figure 1: Overview of bisulfite conversion of DNA</p>
</div>
</div>',
			'in_footer' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'dna-bisulfite-conversion',
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies. Since bisulfite treatment introduces specific changes in the DNA sequence depending on the methylation status of individual cytosine residues',
			'meta_title' => 'Bisulfite sequencing(Bis-Seq) - Bisulfite conversion - DNA Methylation | Diagenode',
			'modified' => '2018-03-14 14:48:31',
			'created' => '2015-02-17 14:48:32',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1',
			'position' => '9',
			'parent_id' => null,
			'name' => 'DNA Methylation',
			'description' => '<div class="row">
<div class="large-12 columns">
<div style="text-align: justify;" class="small-12 medium-8 large-8 columns">
<h2>Complete solutions for DNA methylation studies</h2>
<p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p>
</div>
<div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div>
<div style="text-align: justify;" class="small-12 medium-12 large-12 columns">
<p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p>
</div>
<div class="small-12 medium-12 large-12 columns"><br /><br />
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a>
<div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.
<p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p>
</div>
</li>
</ul>
<br />
<h2>Main DNA methylation technologies</h2>
<p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p>
<div class="row">
<ol>
<li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite &ndash; Bisulfite conversion</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li>
</ol>
<p><span style="font-weight: 400;">&nbsp;</span></p>
<div class="row">
<table>
<thead>
<tr>
<th></th>
<th>Description</th>
<th width="350">Features</th>
</tr>
</thead>
<tbody>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><b>Methylated DNA enrichment</b></td>
<td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><strong>Restriction enzyme-based digestion</strong></td>
<td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div class="row"></div>
</div>
</div>
<div class="large-12 columns"></div>
</div>',
			'in_footer' => true,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'epigenetics-dna-methylation',
			'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation',
			'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.',
			'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode',
			'modified' => '2019-03-25 10:07:27',
			'created' => '2015-05-03 13:47:53',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '54',
			'position' => '1',
			'parent_id' => '12',
			'name' => 'Bisulfite conversion',
			'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Sodium bisulfite conversion of genomic DNA is the most commonly used&nbsp;method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. It enables <span>to differentiate and detect unmethylated versus methylated cytosines.&nbsp;This procedure can then be followed either by <strong>PCR amplification</strong> or&nbsp;<strong>next generation sequencing</strong> to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.</span></p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<h2>How it works</h2>
<p style="text-align: left;">Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged.&nbsp;<span>The DNA is then amplified by PCR where the uracils are converted to thymines.&nbsp;</span></p>
<p style="text-align: center;"><span></span></p>
<p><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/bisulfite-conversion-acgautac.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<h2>Advantages</h2>
<ul class="nobullet" style="font-size: 19px;">
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;</strong><strong>Single nucleotide</strong> resolution</li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;Gene-specific&nbsp;</strong>and&nbsp;<strong>genome-wide</strong><span>&nbsp;analyses</span></li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;NGS</strong><span>&nbsp;</span>compatible</li>
</ul>
<h2>Downstream analysis techniques</h2>
<ul class="square">
<li>Reduced Representation Bisulfite Sequencing (RRBS) with our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS Kit&nbsp;V2</a></li>
<li>Bisulfite conversion with our <a href="https://www.diagenode.com/en/p/premium-bisulfite-kit-50-rxns">Premium Bisulfite Kit</a> followed by qPCR, Sanger, Pyrosequencing</li>
</ul>
<p></p>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'bisulfite-conversion',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfitre conversion is the gold standard method for DNA methylation studies at a single base pair resolution. Prepare your libraries for bisulfite sequencing with one of our Premium kits.',
			'meta_title' => 'DNA Methylation - Bisulfite conversion - Epigenetics | Diagenode ',
			'modified' => '2023-06-20 18:19:50',
			'created' => '2015-07-08 09:42:42',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '101',
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<ul>
<li>
<p><span>Fastest method for complete bisulfite conversion of DNA for methylation analysis. </span></p>
</li>
<li>
<p><span>Ready-to-use conversion reagent is added directly to DNA. </span></p>
</li>
<li>
<p><span>High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen Sequencing. </span></p>
</li>
</ul>
</div>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Manual',
			'url' => 'files/products/kits/Auto-Premium-Bisulfite-kit-manual.pdf',
			'slug' => 'auto-premium-bisulfite-kit-manual',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-09-01 12:36:09',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '1176',
			'name' => 'Auto Premium Bisulfite kit SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-US-en-1_0.pdf',
			'countries' => 'US',
			'modified' => '2021-02-09 11:00:57',
			'created' => '2021-02-09 11:00:57',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1174',
			'name' => 'Auto Premium Bisulfite kit SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-GB-en-1_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-02-09 11:00:18',
			'created' => '2021-02-09 11:00:18',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1172',
			'name' => 'Auto Premium Bisulfite kit SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-ES-es-1_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-02-09 10:59:34',
			'created' => '2021-02-09 10:59:34',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1171',
			'name' => 'Auto Premium Bisulfite kit SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-DE-de-1_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-02-09 10:59:13',
			'created' => '2021-02-09 10:59:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1175',
			'name' => 'Auto Premium Bisulfite kit SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-JP-ja-1_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-02-09 11:00:39',
			'created' => '2021-02-09 11:00:39',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '1170',
			'name' => 'Auto Premium Bisulfite kit SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-BE-nl-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-02-09 10:58:51',
			'created' => '2021-02-09 10:58:51',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '1169',
			'name' => 'Auto Premium Bisulfite kit SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-BE-fr-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-02-09 10:58:33',
			'created' => '2021-02-09 10:58:33',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '1173',
			'name' => 'Auto Premium Bisulfite kit SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-FR-fr-1_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-02-09 10:59:55',
			'created' => '2021-02-09 10:59:55',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-ipure-kit-v2-x100-100-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C03010010</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1899" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1899" id="CartAdd/1899Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1899" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto IPure kit v2</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto IPure kit v2',
                    'C03010010',
                     '410',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto IPure kit v2',
                    'C03010010',
                    '410',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-ipure-kit-v2-x100-100-rxns" data-reveal-id="cartModal-1899" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto IPure kit v2</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02010034</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1885" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1885" id="CartAdd/1885Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1885" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto hMeDIP kit x16 (monoclonal mouse antibody)</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010034',
                     '690',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010034',
                    '690',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1885" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto hMeDIP kit x16 (monoclonal mouse antibody)</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020011</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1888" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1888" id="CartAdd/1888Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1888" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto MethylCap kit</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                     '695',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                    '695',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-methylcap-kit-x48-48-rxns" data-reveal-id="cartModal-1888" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto MethylCap kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x24-24-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030032</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (24 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x96-96-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030033</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (96 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '1895',
	'antibody_id' => null,
	'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
	'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
	'label1' => 'Characteristics',
	'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => 'NATURE METHODS',
	'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label3' => '',
	'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'format' => '96 rxns',
	'catalog_number' => 'C02030033',
	'old_catalog_number' => '',
	'sf_code' => 'C02030033-',
	'type' => 'RFR',
	'search_order' => '04-undefined',
	'price_EUR' => '4415',
	'price_USD' => '4385',
	'price_GBP' => '4085',
	'price_JPY' => '769000',
	'price_CNY' => '',
	'price_AUD' => '10965',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => true,
	'featured' => true,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'premium-rrbs-kit-x96-96-rxns',
	'meta_title' => 'Premium RRBS Kit | Diagenode ',
	'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
	'meta_description' => 'Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries to efficiently analyze DNA methylation at the single nucleotide level. More cost effective than Whole Genome Bisulfite Sequencing (WGBS) and with greater coverage than microarray-based methods.',
	'modified' => '2022-12-05 11:22:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsRelated' => array(
		'id' => '3234',
		'product_id' => '1893',
		'related_id' => '1895'
	),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	)
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '101',
	'name' => 'Auto Premium Bisulfite kit',
	'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<ul>
<li>
<p><span>Fastest method for complete bisulfite conversion of DNA for methylation analysis. </span></p>
</li>
<li>
<p><span>Ready-to-use conversion reagent is added directly to DNA. </span></p>
</li>
<li>
<p><span>High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen Sequencing. </span></p>
</li>
</ul>
</div>
</div>
</div>
</div>',
	'image_id' => null,
	'type' => 'Manual',
	'url' => 'files/products/kits/Auto-Premium-Bisulfite-kit-manual.pdf',
	'slug' => 'auto-premium-bisulfite-kit-manual',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2015-09-01 12:36:09',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '177',
		'product_id' => '1893',
		'document_id' => '101'
	)
)
$sds = array(
	'id' => '1173',
	'name' => 'Auto Premium Bisulfite kit SDS FR fr',
	'language' => 'fr',
	'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-FR-fr-1_0.pdf',
	'countries' => 'FR',
	'modified' => '2021-02-09 10:59:55',
	'created' => '2021-02-09 10:59:55',
	'ProductsSafetySheet' => array(
		'id' => '2051',
		'product_id' => '1893',
		'safety_sheet_id' => '1173'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'Auto Premium Bisulfite kit',
	'meta_title' => 'Auto Premium Bisulfite kit',
	'product' => array(
		'Product' => array(
			'id' => '1893',
			'antibody_id' => null,
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => 'Characteristics',
			'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '40 rxns',
			'catalog_number' => 'C02030031',
			'old_catalog_number' => '',
			'sf_code' => 'C02030031-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '255',
			'price_USD' => '240',
			'price_GBP' => '230',
			'price_JPY' => '39945',
			'price_CNY' => '',
			'price_AUD' => '600',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-premium-bisulfite-kit-40-rxns',
			'meta_title' => 'Auto Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Auto Premium Bisulfite kit',
			'modified' => '2019-12-16 10:05:26',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'Auto Premium Bisulfite kit'
$meta_title = 'Auto Premium Bisulfite kit'
$product = array(
	'Product' => array(
		'id' => '1893',
		'antibody_id' => null,
		'name' => 'Auto Premium Bisulfite kit',
		'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
		'label1' => 'Characteristics',
		'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '40 rxns',
		'catalog_number' => 'C02030031',
		'old_catalog_number' => '',
		'sf_code' => 'C02030031-',
		'type' => 'REF',
		'search_order' => '04-undefined',
		'price_EUR' => '255',
		'price_USD' => '240',
		'price_GBP' => '230',
		'price_JPY' => '39945',
		'price_CNY' => '',
		'price_AUD' => '600',
		'country' => 'ALL',
		'except_countries' => 'Japan',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'auto-premium-bisulfite-kit-40-rxns',
		'meta_title' => 'Auto Premium Bisulfite kit',
		'meta_keywords' => '',
		'meta_description' => 'Auto Premium Bisulfite kit',
		'modified' => '2019-12-16 10:05:26',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1899',
			'antibody_id' => null,
			'name' => 'Auto IPure kit v2',
			'description' => '<p><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></p>
<p>The DNA recovery after ChIP or MeDIP might be an issue for specific downstream applications (e.g. Next generation sequencing). Diagenode's IPure kit is the only DNA purification kit that is specifically optimized for extracting very low amounts of DNA after ChIP &amp; MeDIP.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 rxns',
			'catalog_number' => 'C03010010',
			'old_catalog_number' => 'AL-Auto01-0100',
			'sf_code' => 'C03010010-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '380',
			'price_USD' => '410',
			'price_GBP' => '340',
			'price_JPY' => '59525',
			'price_CNY' => '',
			'price_AUD' => '1025',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-ipure-kit-v2-x100-100-rxns',
			'meta_title' => 'Auto IPure kit v2 x100',
			'meta_keywords' => '',
			'meta_description' => 'Auto IPure kit v2 x100',
			'modified' => '2019-02-27 15:19:42',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1885',
			'antibody_id' => null,
			'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented&nbsp;genomic DNA&nbsp;samples for use in genome-wide methylation analysis. It features</span><span>&nbsp;a highly specific monoclonal antibody against&nbsp;</span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes&nbsp;control DNA and primers to assess the effiency of the assay.&nbsp;</span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast,&nbsp;reliable and&nbsp;highly specific.</span></p>',
			'label1' => ' Characteristics',
			'info1' => '<ul style="list-style-type: disc;">
<li><span>Robust enrichment &amp; immunoprecipitation of hydroxymethylated DNA</span></li>
<li>Highly specific monoclonal antibody against 5-hmC<span>&nbsp;for reliable, reproducible results</span></li>
<li>Including&nbsp;control DNA and primers to&nbsp;<span>monitor the&nbsp;efficiency of the assay</span>
<ul style="list-style-type: circle;">
<li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li>
<li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li>
</ul>
</li>
</ul>
<ul style="list-style-type: disc;">
<li>Improved single-tube, magnetic bead-based protocol</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '16 rxns',
			'catalog_number' => 'C02010034',
			'old_catalog_number' => '',
			'sf_code' => 'C02010034-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '630',
			'price_USD' => '690',
			'price_GBP' => '580',
			'price_JPY' => '98690',
			'price_CNY' => '',
			'price_AUD' => '1725',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns',
			'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'meta_keywords' => '',
			'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'modified' => '2021-01-18 10:37:19',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1888',
			'antibody_id' => null,
			'name' => 'Auto MethylCap kit',
			'description' => '<p>The Auto MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. This procedure has been optimized to perform automated immunoprecipitation of chromatin using the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a> enabling highly reproducible results and allowing for high throughput.</p>',
			'label1' => ' Characteristics',
			'info1' => '<ul>
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>Automation compatibility</strong><strong></strong>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>F</strong><strong>igure 1.</strong><span>&nbsp;</span>Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).<br /><strong></strong></p>
</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020011',
			'old_catalog_number' => 'AF-Auto01-0048',
			'sf_code' => 'C02020011-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-methylcap-kit-x48-48-rxns',
			'meta_title' => 'Auto MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'Auto MethylCap kit x48',
			'modified' => '2020-09-17 13:33:12',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1894',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (24 rxns) - replaced by Premium RRBS kit v2 C02030036',
			'description' => '<div class="extra-spaced"><center></center>The kit Premium RRBS (24 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a><span>.</span>&nbsp;</div>
<div class="extra-spaced">For the information about the format 96 rxns - check out <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-x96-96-rxns">Premium RRBS (96 rxns)</a>.&nbsp;</div>
<div class="extra-spaced"></div>
<p style="text-align: justify;"></p>',
			'label1' => '',
			'info1' => '',
			'label2' => 'Nature Methods',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>',
			'label3' => '',
			'info3' => '',
			'format' => '24 rxns',
			'catalog_number' => 'C02030032',
			'old_catalog_number' => '',
			'sf_code' => 'C02030032-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '1560',
			'price_USD' => '1640',
			'price_GBP' => '1445',
			'price_JPY' => '272000',
			'price_CNY' => '',
			'price_AUD' => '4100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x24-24-rxns',
			'meta_title' => 'Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit x24 | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium RRBS kit analyzes DNA methylation at the single nucleotide level for 4 million CpGs.Reduced Representation Bisulfite Sequencing is fast and affordable.',
			'modified' => '2022-06-02 14:31:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1895',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
			'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'NATURE METHODS',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '96 rxns',
			'catalog_number' => 'C02030033',
			'old_catalog_number' => '',
			'sf_code' => 'C02030033-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '4415',
			'price_USD' => '4385',
			'price_GBP' => '4085',
			'price_JPY' => '769000',
			'price_CNY' => '',
			'price_AUD' => '10965',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x96-96-rxns',
			'meta_title' => 'Premium RRBS Kit | Diagenode ',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries to efficiently analyze DNA methylation at the single nucleotide level. More cost effective than Whole Genome Bisulfite Sequencing (WGBS) and with greater coverage than microarray-based methods.',
			'modified' => '2022-12-05 11:22:21',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '5',
			'position' => '10',
			'parent_id' => '1',
			'name' => 'Bisulfite conversion',
			'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">Bisulfite modification of DNA is the most commonly used, "<strong>gold standard</strong>" method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. T<span style="font-weight: 400;">his technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.</span></div>
<div style="text-align: justify;" class="large-12 columns"></div>
<div style="text-align: justify;" class="large-12 columns">Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.
<h3>How it works</h3>
Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).
<p class="text-center"><img src="https://www.diagenode.com/img/applications/bisulfite.png" /><br />Figure 1: Overview of bisulfite conversion of DNA</p>
</div>
</div>',
			'in_footer' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'dna-bisulfite-conversion',
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies. Since bisulfite treatment introduces specific changes in the DNA sequence depending on the methylation status of individual cytosine residues',
			'meta_title' => 'Bisulfite sequencing(Bis-Seq) - Bisulfite conversion - DNA Methylation | Diagenode',
			'modified' => '2018-03-14 14:48:31',
			'created' => '2015-02-17 14:48:32',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1',
			'position' => '9',
			'parent_id' => null,
			'name' => 'DNA Methylation',
			'description' => '<div class="row">
<div class="large-12 columns">
<div style="text-align: justify;" class="small-12 medium-8 large-8 columns">
<h2>Complete solutions for DNA methylation studies</h2>
<p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p>
</div>
<div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div>
<div style="text-align: justify;" class="small-12 medium-12 large-12 columns">
<p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p>
</div>
<div class="small-12 medium-12 large-12 columns"><br /><br />
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a>
<div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.
<p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p>
</div>
</li>
</ul>
<br />
<h2>Main DNA methylation technologies</h2>
<p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p>
<div class="row">
<ol>
<li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite &ndash; Bisulfite conversion</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li>
</ol>
<p><span style="font-weight: 400;">&nbsp;</span></p>
<div class="row">
<table>
<thead>
<tr>
<th></th>
<th>Description</th>
<th width="350">Features</th>
</tr>
</thead>
<tbody>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><b>Methylated DNA enrichment</b></td>
<td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><strong>Restriction enzyme-based digestion</strong></td>
<td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div class="row"></div>
</div>
</div>
<div class="large-12 columns"></div>
</div>',
			'in_footer' => true,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'epigenetics-dna-methylation',
			'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation',
			'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.',
			'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode',
			'modified' => '2019-03-25 10:07:27',
			'created' => '2015-05-03 13:47:53',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '54',
			'position' => '1',
			'parent_id' => '12',
			'name' => 'Bisulfite conversion',
			'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Sodium bisulfite conversion of genomic DNA is the most commonly used&nbsp;method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. It enables <span>to differentiate and detect unmethylated versus methylated cytosines.&nbsp;This procedure can then be followed either by <strong>PCR amplification</strong> or&nbsp;<strong>next generation sequencing</strong> to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.</span></p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<h2>How it works</h2>
<p style="text-align: left;">Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged.&nbsp;<span>The DNA is then amplified by PCR where the uracils are converted to thymines.&nbsp;</span></p>
<p style="text-align: center;"><span></span></p>
<p><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/bisulfite-conversion-acgautac.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<h2>Advantages</h2>
<ul class="nobullet" style="font-size: 19px;">
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;</strong><strong>Single nucleotide</strong> resolution</li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;Gene-specific&nbsp;</strong>and&nbsp;<strong>genome-wide</strong><span>&nbsp;analyses</span></li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;NGS</strong><span>&nbsp;</span>compatible</li>
</ul>
<h2>Downstream analysis techniques</h2>
<ul class="square">
<li>Reduced Representation Bisulfite Sequencing (RRBS) with our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS Kit&nbsp;V2</a></li>
<li>Bisulfite conversion with our <a href="https://www.diagenode.com/en/p/premium-bisulfite-kit-50-rxns">Premium Bisulfite Kit</a> followed by qPCR, Sanger, Pyrosequencing</li>
</ul>
<p></p>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'bisulfite-conversion',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfitre conversion is the gold standard method for DNA methylation studies at a single base pair resolution. Prepare your libraries for bisulfite sequencing with one of our Premium kits.',
			'meta_title' => 'DNA Methylation - Bisulfite conversion - Epigenetics | Diagenode ',
			'modified' => '2023-06-20 18:19:50',
			'created' => '2015-07-08 09:42:42',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '101',
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<ul>
<li>
<p><span>Fastest method for complete bisulfite conversion of DNA for methylation analysis. </span></p>
</li>
<li>
<p><span>Ready-to-use conversion reagent is added directly to DNA. </span></p>
</li>
<li>
<p><span>High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen Sequencing. </span></p>
</li>
</ul>
</div>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Manual',
			'url' => 'files/products/kits/Auto-Premium-Bisulfite-kit-manual.pdf',
			'slug' => 'auto-premium-bisulfite-kit-manual',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-09-01 12:36:09',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '1176',
			'name' => 'Auto Premium Bisulfite kit SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-US-en-1_0.pdf',
			'countries' => 'US',
			'modified' => '2021-02-09 11:00:57',
			'created' => '2021-02-09 11:00:57',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1174',
			'name' => 'Auto Premium Bisulfite kit SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-GB-en-1_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-02-09 11:00:18',
			'created' => '2021-02-09 11:00:18',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1172',
			'name' => 'Auto Premium Bisulfite kit SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-ES-es-1_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-02-09 10:59:34',
			'created' => '2021-02-09 10:59:34',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1171',
			'name' => 'Auto Premium Bisulfite kit SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-DE-de-1_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-02-09 10:59:13',
			'created' => '2021-02-09 10:59:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1175',
			'name' => 'Auto Premium Bisulfite kit SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-JP-ja-1_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-02-09 11:00:39',
			'created' => '2021-02-09 11:00:39',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '1170',
			'name' => 'Auto Premium Bisulfite kit SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-BE-nl-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-02-09 10:58:51',
			'created' => '2021-02-09 10:58:51',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '1169',
			'name' => 'Auto Premium Bisulfite kit SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-BE-fr-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-02-09 10:58:33',
			'created' => '2021-02-09 10:58:33',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '1173',
			'name' => 'Auto Premium Bisulfite kit SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-FR-fr-1_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-02-09 10:59:55',
			'created' => '2021-02-09 10:59:55',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-ipure-kit-v2-x100-100-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C03010010</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1899" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1899" id="CartAdd/1899Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1899" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto IPure kit v2</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto IPure kit v2',
                    'C03010010',
                     '410',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto IPure kit v2',
                    'C03010010',
                    '410',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-ipure-kit-v2-x100-100-rxns" data-reveal-id="cartModal-1899" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto IPure kit v2</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02010034</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1885" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1885" id="CartAdd/1885Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1885" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto hMeDIP kit x16 (monoclonal mouse antibody)</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010034',
                     '690',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010034',
                    '690',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1885" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto hMeDIP kit x16 (monoclonal mouse antibody)</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020011</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1888" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1888" id="CartAdd/1888Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1888" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto MethylCap kit</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                     '695',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                    '695',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-methylcap-kit-x48-48-rxns" data-reveal-id="cartModal-1888" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto MethylCap kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x24-24-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030032</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (24 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x96-96-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030033</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (96 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '1895',
	'antibody_id' => null,
	'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
	'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
	'label1' => 'Characteristics',
	'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => 'NATURE METHODS',
	'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label3' => '',
	'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'format' => '96 rxns',
	'catalog_number' => 'C02030033',
	'old_catalog_number' => '',
	'sf_code' => 'C02030033-',
	'type' => 'RFR',
	'search_order' => '04-undefined',
	'price_EUR' => '4415',
	'price_USD' => '4385',
	'price_GBP' => '4085',
	'price_JPY' => '769000',
	'price_CNY' => '',
	'price_AUD' => '10965',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => true,
	'featured' => true,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'premium-rrbs-kit-x96-96-rxns',
	'meta_title' => 'Premium RRBS Kit | Diagenode ',
	'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
	'meta_description' => 'Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries to efficiently analyze DNA methylation at the single nucleotide level. More cost effective than Whole Genome Bisulfite Sequencing (WGBS) and with greater coverage than microarray-based methods.',
	'modified' => '2022-12-05 11:22:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsRelated' => array(
		'id' => '3234',
		'product_id' => '1893',
		'related_id' => '1895'
	),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	)
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '101',
	'name' => 'Auto Premium Bisulfite kit',
	'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<ul>
<li>
<p><span>Fastest method for complete bisulfite conversion of DNA for methylation analysis. </span></p>
</li>
<li>
<p><span>Ready-to-use conversion reagent is added directly to DNA. </span></p>
</li>
<li>
<p><span>High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen Sequencing. </span></p>
</li>
</ul>
</div>
</div>
</div>
</div>',
	'image_id' => null,
	'type' => 'Manual',
	'url' => 'files/products/kits/Auto-Premium-Bisulfite-kit-manual.pdf',
	'slug' => 'auto-premium-bisulfite-kit-manual',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2015-09-01 12:36:09',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '177',
		'product_id' => '1893',
		'document_id' => '101'
	)
)
$sds = array(
	'id' => '1173',
	'name' => 'Auto Premium Bisulfite kit SDS FR fr',
	'language' => 'fr',
	'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-FR-fr-1_0.pdf',
	'countries' => 'FR',
	'modified' => '2021-02-09 10:59:55',
	'created' => '2021-02-09 10:59:55',
	'ProductsSafetySheet' => array(
		'id' => '2051',
		'product_id' => '1893',
		'safety_sheet_id' => '1173'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'Auto Premium Bisulfite kit',
	'meta_title' => 'Auto Premium Bisulfite kit',
	'product' => array(
		'Product' => array(
			'id' => '1893',
			'antibody_id' => null,
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => 'Characteristics',
			'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '40 rxns',
			'catalog_number' => 'C02030031',
			'old_catalog_number' => '',
			'sf_code' => 'C02030031-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '255',
			'price_USD' => '240',
			'price_GBP' => '230',
			'price_JPY' => '39945',
			'price_CNY' => '',
			'price_AUD' => '600',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-premium-bisulfite-kit-40-rxns',
			'meta_title' => 'Auto Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Auto Premium Bisulfite kit',
			'modified' => '2019-12-16 10:05:26',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'Auto Premium Bisulfite kit'
$meta_title = 'Auto Premium Bisulfite kit'
$product = array(
	'Product' => array(
		'id' => '1893',
		'antibody_id' => null,
		'name' => 'Auto Premium Bisulfite kit',
		'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
		'label1' => 'Characteristics',
		'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '40 rxns',
		'catalog_number' => 'C02030031',
		'old_catalog_number' => '',
		'sf_code' => 'C02030031-',
		'type' => 'REF',
		'search_order' => '04-undefined',
		'price_EUR' => '255',
		'price_USD' => '240',
		'price_GBP' => '230',
		'price_JPY' => '39945',
		'price_CNY' => '',
		'price_AUD' => '600',
		'country' => 'ALL',
		'except_countries' => 'Japan',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'auto-premium-bisulfite-kit-40-rxns',
		'meta_title' => 'Auto Premium Bisulfite kit',
		'meta_keywords' => '',
		'meta_description' => 'Auto Premium Bisulfite kit',
		'modified' => '2019-12-16 10:05:26',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1899',
			'antibody_id' => null,
			'name' => 'Auto IPure kit v2',
			'description' => '<p><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></p>
<p>The DNA recovery after ChIP or MeDIP might be an issue for specific downstream applications (e.g. Next generation sequencing). Diagenode's IPure kit is the only DNA purification kit that is specifically optimized for extracting very low amounts of DNA after ChIP &amp; MeDIP.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 rxns',
			'catalog_number' => 'C03010010',
			'old_catalog_number' => 'AL-Auto01-0100',
			'sf_code' => 'C03010010-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '380',
			'price_USD' => '410',
			'price_GBP' => '340',
			'price_JPY' => '59525',
			'price_CNY' => '',
			'price_AUD' => '1025',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-ipure-kit-v2-x100-100-rxns',
			'meta_title' => 'Auto IPure kit v2 x100',
			'meta_keywords' => '',
			'meta_description' => 'Auto IPure kit v2 x100',
			'modified' => '2019-02-27 15:19:42',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1885',
			'antibody_id' => null,
			'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented&nbsp;genomic DNA&nbsp;samples for use in genome-wide methylation analysis. It features</span><span>&nbsp;a highly specific monoclonal antibody against&nbsp;</span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes&nbsp;control DNA and primers to assess the effiency of the assay.&nbsp;</span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast,&nbsp;reliable and&nbsp;highly specific.</span></p>',
			'label1' => ' Characteristics',
			'info1' => '<ul style="list-style-type: disc;">
<li><span>Robust enrichment &amp; immunoprecipitation of hydroxymethylated DNA</span></li>
<li>Highly specific monoclonal antibody against 5-hmC<span>&nbsp;for reliable, reproducible results</span></li>
<li>Including&nbsp;control DNA and primers to&nbsp;<span>monitor the&nbsp;efficiency of the assay</span>
<ul style="list-style-type: circle;">
<li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li>
<li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li>
</ul>
</li>
</ul>
<ul style="list-style-type: disc;">
<li>Improved single-tube, magnetic bead-based protocol</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '16 rxns',
			'catalog_number' => 'C02010034',
			'old_catalog_number' => '',
			'sf_code' => 'C02010034-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '630',
			'price_USD' => '690',
			'price_GBP' => '580',
			'price_JPY' => '98690',
			'price_CNY' => '',
			'price_AUD' => '1725',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns',
			'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'meta_keywords' => '',
			'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'modified' => '2021-01-18 10:37:19',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1888',
			'antibody_id' => null,
			'name' => 'Auto MethylCap kit',
			'description' => '<p>The Auto MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. This procedure has been optimized to perform automated immunoprecipitation of chromatin using the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a> enabling highly reproducible results and allowing for high throughput.</p>',
			'label1' => ' Characteristics',
			'info1' => '<ul>
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>Automation compatibility</strong><strong></strong>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>F</strong><strong>igure 1.</strong><span>&nbsp;</span>Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).<br /><strong></strong></p>
</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020011',
			'old_catalog_number' => 'AF-Auto01-0048',
			'sf_code' => 'C02020011-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-methylcap-kit-x48-48-rxns',
			'meta_title' => 'Auto MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'Auto MethylCap kit x48',
			'modified' => '2020-09-17 13:33:12',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1894',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (24 rxns) - replaced by Premium RRBS kit v2 C02030036',
			'description' => '<div class="extra-spaced"><center></center>The kit Premium RRBS (24 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a><span>.</span>&nbsp;</div>
<div class="extra-spaced">For the information about the format 96 rxns - check out <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-x96-96-rxns">Premium RRBS (96 rxns)</a>.&nbsp;</div>
<div class="extra-spaced"></div>
<p style="text-align: justify;"></p>',
			'label1' => '',
			'info1' => '',
			'label2' => 'Nature Methods',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>',
			'label3' => '',
			'info3' => '',
			'format' => '24 rxns',
			'catalog_number' => 'C02030032',
			'old_catalog_number' => '',
			'sf_code' => 'C02030032-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '1560',
			'price_USD' => '1640',
			'price_GBP' => '1445',
			'price_JPY' => '272000',
			'price_CNY' => '',
			'price_AUD' => '4100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x24-24-rxns',
			'meta_title' => 'Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit x24 | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium RRBS kit analyzes DNA methylation at the single nucleotide level for 4 million CpGs.Reduced Representation Bisulfite Sequencing is fast and affordable.',
			'modified' => '2022-06-02 14:31:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1895',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
			'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'NATURE METHODS',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '96 rxns',
			'catalog_number' => 'C02030033',
			'old_catalog_number' => '',
			'sf_code' => 'C02030033-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '4415',
			'price_USD' => '4385',
			'price_GBP' => '4085',
			'price_JPY' => '769000',
			'price_CNY' => '',
			'price_AUD' => '10965',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x96-96-rxns',
			'meta_title' => 'Premium RRBS Kit | Diagenode ',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries to efficiently analyze DNA methylation at the single nucleotide level. More cost effective than Whole Genome Bisulfite Sequencing (WGBS) and with greater coverage than microarray-based methods.',
			'modified' => '2022-12-05 11:22:21',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '5',
			'position' => '10',
			'parent_id' => '1',
			'name' => 'Bisulfite conversion',
			'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">Bisulfite modification of DNA is the most commonly used, "<strong>gold standard</strong>" method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. T<span style="font-weight: 400;">his technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.</span></div>
<div style="text-align: justify;" class="large-12 columns"></div>
<div style="text-align: justify;" class="large-12 columns">Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.
<h3>How it works</h3>
Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).
<p class="text-center"><img src="https://www.diagenode.com/img/applications/bisulfite.png" /><br />Figure 1: Overview of bisulfite conversion of DNA</p>
</div>
</div>',
			'in_footer' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'dna-bisulfite-conversion',
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies. Since bisulfite treatment introduces specific changes in the DNA sequence depending on the methylation status of individual cytosine residues',
			'meta_title' => 'Bisulfite sequencing(Bis-Seq) - Bisulfite conversion - DNA Methylation | Diagenode',
			'modified' => '2018-03-14 14:48:31',
			'created' => '2015-02-17 14:48:32',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1',
			'position' => '9',
			'parent_id' => null,
			'name' => 'DNA Methylation',
			'description' => '<div class="row">
<div class="large-12 columns">
<div style="text-align: justify;" class="small-12 medium-8 large-8 columns">
<h2>Complete solutions for DNA methylation studies</h2>
<p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p>
</div>
<div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div>
<div style="text-align: justify;" class="small-12 medium-12 large-12 columns">
<p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p>
</div>
<div class="small-12 medium-12 large-12 columns"><br /><br />
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a>
<div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.
<p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p>
</div>
</li>
</ul>
<br />
<h2>Main DNA methylation technologies</h2>
<p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p>
<div class="row">
<ol>
<li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite &ndash; Bisulfite conversion</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li>
</ol>
<p><span style="font-weight: 400;">&nbsp;</span></p>
<div class="row">
<table>
<thead>
<tr>
<th></th>
<th>Description</th>
<th width="350">Features</th>
</tr>
</thead>
<tbody>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><b>Methylated DNA enrichment</b></td>
<td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><strong>Restriction enzyme-based digestion</strong></td>
<td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div class="row"></div>
</div>
</div>
<div class="large-12 columns"></div>
</div>',
			'in_footer' => true,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'epigenetics-dna-methylation',
			'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation',
			'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.',
			'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode',
			'modified' => '2019-03-25 10:07:27',
			'created' => '2015-05-03 13:47:53',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '54',
			'position' => '1',
			'parent_id' => '12',
			'name' => 'Bisulfite conversion',
			'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Sodium bisulfite conversion of genomic DNA is the most commonly used&nbsp;method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. It enables <span>to differentiate and detect unmethylated versus methylated cytosines.&nbsp;This procedure can then be followed either by <strong>PCR amplification</strong> or&nbsp;<strong>next generation sequencing</strong> to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.</span></p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<h2>How it works</h2>
<p style="text-align: left;">Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged.&nbsp;<span>The DNA is then amplified by PCR where the uracils are converted to thymines.&nbsp;</span></p>
<p style="text-align: center;"><span></span></p>
<p><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/bisulfite-conversion-acgautac.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<h2>Advantages</h2>
<ul class="nobullet" style="font-size: 19px;">
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;</strong><strong>Single nucleotide</strong> resolution</li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;Gene-specific&nbsp;</strong>and&nbsp;<strong>genome-wide</strong><span>&nbsp;analyses</span></li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;NGS</strong><span>&nbsp;</span>compatible</li>
</ul>
<h2>Downstream analysis techniques</h2>
<ul class="square">
<li>Reduced Representation Bisulfite Sequencing (RRBS) with our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS Kit&nbsp;V2</a></li>
<li>Bisulfite conversion with our <a href="https://www.diagenode.com/en/p/premium-bisulfite-kit-50-rxns">Premium Bisulfite Kit</a> followed by qPCR, Sanger, Pyrosequencing</li>
</ul>
<p></p>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'bisulfite-conversion',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfitre conversion is the gold standard method for DNA methylation studies at a single base pair resolution. Prepare your libraries for bisulfite sequencing with one of our Premium kits.',
			'meta_title' => 'DNA Methylation - Bisulfite conversion - Epigenetics | Diagenode ',
			'modified' => '2023-06-20 18:19:50',
			'created' => '2015-07-08 09:42:42',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '101',
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<ul>
<li>
<p><span>Fastest method for complete bisulfite conversion of DNA for methylation analysis. </span></p>
</li>
<li>
<p><span>Ready-to-use conversion reagent is added directly to DNA. </span></p>
</li>
<li>
<p><span>High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen Sequencing. </span></p>
</li>
</ul>
</div>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Manual',
			'url' => 'files/products/kits/Auto-Premium-Bisulfite-kit-manual.pdf',
			'slug' => 'auto-premium-bisulfite-kit-manual',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-09-01 12:36:09',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '1176',
			'name' => 'Auto Premium Bisulfite kit SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-US-en-1_0.pdf',
			'countries' => 'US',
			'modified' => '2021-02-09 11:00:57',
			'created' => '2021-02-09 11:00:57',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1174',
			'name' => 'Auto Premium Bisulfite kit SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-GB-en-1_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-02-09 11:00:18',
			'created' => '2021-02-09 11:00:18',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1172',
			'name' => 'Auto Premium Bisulfite kit SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-ES-es-1_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-02-09 10:59:34',
			'created' => '2021-02-09 10:59:34',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1171',
			'name' => 'Auto Premium Bisulfite kit SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-DE-de-1_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-02-09 10:59:13',
			'created' => '2021-02-09 10:59:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1175',
			'name' => 'Auto Premium Bisulfite kit SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-JP-ja-1_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-02-09 11:00:39',
			'created' => '2021-02-09 11:00:39',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '1170',
			'name' => 'Auto Premium Bisulfite kit SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-BE-nl-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-02-09 10:58:51',
			'created' => '2021-02-09 10:58:51',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '1169',
			'name' => 'Auto Premium Bisulfite kit SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-BE-fr-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-02-09 10:58:33',
			'created' => '2021-02-09 10:58:33',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '1173',
			'name' => 'Auto Premium Bisulfite kit SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-FR-fr-1_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-02-09 10:59:55',
			'created' => '2021-02-09 10:59:55',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-ipure-kit-v2-x100-100-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C03010010</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1899" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1899" id="CartAdd/1899Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1899" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto IPure kit v2</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto IPure kit v2',
                    'C03010010',
                     '410',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto IPure kit v2',
                    'C03010010',
                    '410',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-ipure-kit-v2-x100-100-rxns" data-reveal-id="cartModal-1899" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto IPure kit v2</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02010034</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1885" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1885" id="CartAdd/1885Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1885" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto hMeDIP kit x16 (monoclonal mouse antibody)</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010034',
                     '690',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010034',
                    '690',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1885" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto hMeDIP kit x16 (monoclonal mouse antibody)</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020011</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1888" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1888" id="CartAdd/1888Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1888" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto MethylCap kit</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                     '695',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                    '695',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-methylcap-kit-x48-48-rxns" data-reveal-id="cartModal-1888" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto MethylCap kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x24-24-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030032</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (24 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x96-96-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030033</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (96 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '1895',
	'antibody_id' => null,
	'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
	'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
	'label1' => 'Characteristics',
	'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => 'NATURE METHODS',
	'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label3' => '',
	'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'format' => '96 rxns',
	'catalog_number' => 'C02030033',
	'old_catalog_number' => '',
	'sf_code' => 'C02030033-',
	'type' => 'RFR',
	'search_order' => '04-undefined',
	'price_EUR' => '4415',
	'price_USD' => '4385',
	'price_GBP' => '4085',
	'price_JPY' => '769000',
	'price_CNY' => '',
	'price_AUD' => '10965',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => true,
	'featured' => true,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'premium-rrbs-kit-x96-96-rxns',
	'meta_title' => 'Premium RRBS Kit | Diagenode ',
	'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
	'meta_description' => 'Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries to efficiently analyze DNA methylation at the single nucleotide level. More cost effective than Whole Genome Bisulfite Sequencing (WGBS) and with greater coverage than microarray-based methods.',
	'modified' => '2022-12-05 11:22:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsRelated' => array(
		'id' => '3234',
		'product_id' => '1893',
		'related_id' => '1895'
	),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	)
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '101',
	'name' => 'Auto Premium Bisulfite kit',
	'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<ul>
<li>
<p><span>Fastest method for complete bisulfite conversion of DNA for methylation analysis. </span></p>
</li>
<li>
<p><span>Ready-to-use conversion reagent is added directly to DNA. </span></p>
</li>
<li>
<p><span>High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen Sequencing. </span></p>
</li>
</ul>
</div>
</div>
</div>
</div>',
	'image_id' => null,
	'type' => 'Manual',
	'url' => 'files/products/kits/Auto-Premium-Bisulfite-kit-manual.pdf',
	'slug' => 'auto-premium-bisulfite-kit-manual',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2015-09-01 12:36:09',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '177',
		'product_id' => '1893',
		'document_id' => '101'
	)
)
$sds = array(
	'id' => '1173',
	'name' => 'Auto Premium Bisulfite kit SDS FR fr',
	'language' => 'fr',
	'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-FR-fr-1_0.pdf',
	'countries' => 'FR',
	'modified' => '2021-02-09 10:59:55',
	'created' => '2021-02-09 10:59:55',
	'ProductsSafetySheet' => array(
		'id' => '2051',
		'product_id' => '1893',
		'safety_sheet_id' => '1173'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'Auto Premium Bisulfite kit',
	'meta_title' => 'Auto Premium Bisulfite kit',
	'product' => array(
		'Product' => array(
			'id' => '1893',
			'antibody_id' => null,
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => 'Characteristics',
			'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '40 rxns',
			'catalog_number' => 'C02030031',
			'old_catalog_number' => '',
			'sf_code' => 'C02030031-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '255',
			'price_USD' => '240',
			'price_GBP' => '230',
			'price_JPY' => '39945',
			'price_CNY' => '',
			'price_AUD' => '600',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-premium-bisulfite-kit-40-rxns',
			'meta_title' => 'Auto Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Auto Premium Bisulfite kit',
			'modified' => '2019-12-16 10:05:26',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'Auto Premium Bisulfite kit'
$meta_title = 'Auto Premium Bisulfite kit'
$product = array(
	'Product' => array(
		'id' => '1893',
		'antibody_id' => null,
		'name' => 'Auto Premium Bisulfite kit',
		'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
		'label1' => 'Characteristics',
		'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '40 rxns',
		'catalog_number' => 'C02030031',
		'old_catalog_number' => '',
		'sf_code' => 'C02030031-',
		'type' => 'REF',
		'search_order' => '04-undefined',
		'price_EUR' => '255',
		'price_USD' => '240',
		'price_GBP' => '230',
		'price_JPY' => '39945',
		'price_CNY' => '',
		'price_AUD' => '600',
		'country' => 'ALL',
		'except_countries' => 'Japan',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'auto-premium-bisulfite-kit-40-rxns',
		'meta_title' => 'Auto Premium Bisulfite kit',
		'meta_keywords' => '',
		'meta_description' => 'Auto Premium Bisulfite kit',
		'modified' => '2019-12-16 10:05:26',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1899',
			'antibody_id' => null,
			'name' => 'Auto IPure kit v2',
			'description' => '<p><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></p>
<p>The DNA recovery after ChIP or MeDIP might be an issue for specific downstream applications (e.g. Next generation sequencing). Diagenode's IPure kit is the only DNA purification kit that is specifically optimized for extracting very low amounts of DNA after ChIP &amp; MeDIP.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 rxns',
			'catalog_number' => 'C03010010',
			'old_catalog_number' => 'AL-Auto01-0100',
			'sf_code' => 'C03010010-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '380',
			'price_USD' => '410',
			'price_GBP' => '340',
			'price_JPY' => '59525',
			'price_CNY' => '',
			'price_AUD' => '1025',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-ipure-kit-v2-x100-100-rxns',
			'meta_title' => 'Auto IPure kit v2 x100',
			'meta_keywords' => '',
			'meta_description' => 'Auto IPure kit v2 x100',
			'modified' => '2019-02-27 15:19:42',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1885',
			'antibody_id' => null,
			'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented&nbsp;genomic DNA&nbsp;samples for use in genome-wide methylation analysis. It features</span><span>&nbsp;a highly specific monoclonal antibody against&nbsp;</span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes&nbsp;control DNA and primers to assess the effiency of the assay.&nbsp;</span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast,&nbsp;reliable and&nbsp;highly specific.</span></p>',
			'label1' => ' Characteristics',
			'info1' => '<ul style="list-style-type: disc;">
<li><span>Robust enrichment &amp; immunoprecipitation of hydroxymethylated DNA</span></li>
<li>Highly specific monoclonal antibody against 5-hmC<span>&nbsp;for reliable, reproducible results</span></li>
<li>Including&nbsp;control DNA and primers to&nbsp;<span>monitor the&nbsp;efficiency of the assay</span>
<ul style="list-style-type: circle;">
<li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li>
<li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li>
</ul>
</li>
</ul>
<ul style="list-style-type: disc;">
<li>Improved single-tube, magnetic bead-based protocol</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '16 rxns',
			'catalog_number' => 'C02010034',
			'old_catalog_number' => '',
			'sf_code' => 'C02010034-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '630',
			'price_USD' => '690',
			'price_GBP' => '580',
			'price_JPY' => '98690',
			'price_CNY' => '',
			'price_AUD' => '1725',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns',
			'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'meta_keywords' => '',
			'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'modified' => '2021-01-18 10:37:19',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1888',
			'antibody_id' => null,
			'name' => 'Auto MethylCap kit',
			'description' => '<p>The Auto MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. This procedure has been optimized to perform automated immunoprecipitation of chromatin using the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a> enabling highly reproducible results and allowing for high throughput.</p>',
			'label1' => ' Characteristics',
			'info1' => '<ul>
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>Automation compatibility</strong><strong></strong>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>F</strong><strong>igure 1.</strong><span>&nbsp;</span>Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).<br /><strong></strong></p>
</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020011',
			'old_catalog_number' => 'AF-Auto01-0048',
			'sf_code' => 'C02020011-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-methylcap-kit-x48-48-rxns',
			'meta_title' => 'Auto MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'Auto MethylCap kit x48',
			'modified' => '2020-09-17 13:33:12',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1894',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (24 rxns) - replaced by Premium RRBS kit v2 C02030036',
			'description' => '<div class="extra-spaced"><center></center>The kit Premium RRBS (24 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a><span>.</span>&nbsp;</div>
<div class="extra-spaced">For the information about the format 96 rxns - check out <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-x96-96-rxns">Premium RRBS (96 rxns)</a>.&nbsp;</div>
<div class="extra-spaced"></div>
<p style="text-align: justify;"></p>',
			'label1' => '',
			'info1' => '',
			'label2' => 'Nature Methods',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>',
			'label3' => '',
			'info3' => '',
			'format' => '24 rxns',
			'catalog_number' => 'C02030032',
			'old_catalog_number' => '',
			'sf_code' => 'C02030032-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '1560',
			'price_USD' => '1640',
			'price_GBP' => '1445',
			'price_JPY' => '272000',
			'price_CNY' => '',
			'price_AUD' => '4100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x24-24-rxns',
			'meta_title' => 'Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit x24 | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium RRBS kit analyzes DNA methylation at the single nucleotide level for 4 million CpGs.Reduced Representation Bisulfite Sequencing is fast and affordable.',
			'modified' => '2022-06-02 14:31:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1895',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
			'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'NATURE METHODS',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '96 rxns',
			'catalog_number' => 'C02030033',
			'old_catalog_number' => '',
			'sf_code' => 'C02030033-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '4415',
			'price_USD' => '4385',
			'price_GBP' => '4085',
			'price_JPY' => '769000',
			'price_CNY' => '',
			'price_AUD' => '10965',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x96-96-rxns',
			'meta_title' => 'Premium RRBS Kit | Diagenode ',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries to efficiently analyze DNA methylation at the single nucleotide level. More cost effective than Whole Genome Bisulfite Sequencing (WGBS) and with greater coverage than microarray-based methods.',
			'modified' => '2022-12-05 11:22:21',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '5',
			'position' => '10',
			'parent_id' => '1',
			'name' => 'Bisulfite conversion',
			'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">Bisulfite modification of DNA is the most commonly used, "<strong>gold standard</strong>" method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. T<span style="font-weight: 400;">his technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.</span></div>
<div style="text-align: justify;" class="large-12 columns"></div>
<div style="text-align: justify;" class="large-12 columns">Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.
<h3>How it works</h3>
Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).
<p class="text-center"><img src="https://www.diagenode.com/img/applications/bisulfite.png" /><br />Figure 1: Overview of bisulfite conversion of DNA</p>
</div>
</div>',
			'in_footer' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'dna-bisulfite-conversion',
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies. Since bisulfite treatment introduces specific changes in the DNA sequence depending on the methylation status of individual cytosine residues',
			'meta_title' => 'Bisulfite sequencing(Bis-Seq) - Bisulfite conversion - DNA Methylation | Diagenode',
			'modified' => '2018-03-14 14:48:31',
			'created' => '2015-02-17 14:48:32',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1',
			'position' => '9',
			'parent_id' => null,
			'name' => 'DNA Methylation',
			'description' => '<div class="row">
<div class="large-12 columns">
<div style="text-align: justify;" class="small-12 medium-8 large-8 columns">
<h2>Complete solutions for DNA methylation studies</h2>
<p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p>
</div>
<div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div>
<div style="text-align: justify;" class="small-12 medium-12 large-12 columns">
<p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p>
</div>
<div class="small-12 medium-12 large-12 columns"><br /><br />
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a>
<div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.
<p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p>
</div>
</li>
</ul>
<br />
<h2>Main DNA methylation technologies</h2>
<p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p>
<div class="row">
<ol>
<li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite &ndash; Bisulfite conversion</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li>
</ol>
<p><span style="font-weight: 400;">&nbsp;</span></p>
<div class="row">
<table>
<thead>
<tr>
<th></th>
<th>Description</th>
<th width="350">Features</th>
</tr>
</thead>
<tbody>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><b>Methylated DNA enrichment</b></td>
<td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><strong>Restriction enzyme-based digestion</strong></td>
<td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div class="row"></div>
</div>
</div>
<div class="large-12 columns"></div>
</div>',
			'in_footer' => true,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'epigenetics-dna-methylation',
			'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation',
			'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.',
			'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode',
			'modified' => '2019-03-25 10:07:27',
			'created' => '2015-05-03 13:47:53',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '54',
			'position' => '1',
			'parent_id' => '12',
			'name' => 'Bisulfite conversion',
			'description' => '<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p>Sodium bisulfite conversion of genomic DNA is the most commonly used&nbsp;method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. It enables <span>to differentiate and detect unmethylated versus methylated cytosines.&nbsp;This procedure can then be followed either by <strong>PCR amplification</strong> or&nbsp;<strong>next generation sequencing</strong> to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.</span></p>
</div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
 var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } 
// ]]></script>
</center></div>
</div>
<h2>How it works</h2>
<p style="text-align: left;">Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged.&nbsp;<span>The DNA is then amplified by PCR where the uracils are converted to thymines.&nbsp;</span></p>
<p style="text-align: center;"><span></span></p>
<p><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/bisulfite-conversion-acgautac.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<h2>Advantages</h2>
<ul class="nobullet" style="font-size: 19px;">
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;</strong><strong>Single nucleotide</strong> resolution</li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;Gene-specific&nbsp;</strong>and&nbsp;<strong>genome-wide</strong><span>&nbsp;analyses</span></li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;NGS</strong><span>&nbsp;</span>compatible</li>
</ul>
<h2>Downstream analysis techniques</h2>
<ul class="square">
<li>Reduced Representation Bisulfite Sequencing (RRBS) with our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS Kit&nbsp;V2</a></li>
<li>Bisulfite conversion with our <a href="https://www.diagenode.com/en/p/premium-bisulfite-kit-50-rxns">Premium Bisulfite Kit</a> followed by qPCR, Sanger, Pyrosequencing</li>
</ul>
<p></p>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'bisulfite-conversion',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfitre conversion is the gold standard method for DNA methylation studies at a single base pair resolution. Prepare your libraries for bisulfite sequencing with one of our Premium kits.',
			'meta_title' => 'DNA Methylation - Bisulfite conversion - Epigenetics | Diagenode ',
			'modified' => '2023-06-20 18:19:50',
			'created' => '2015-07-08 09:42:42',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '101',
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<ul>
<li>
<p><span>Fastest method for complete bisulfite conversion of DNA for methylation analysis. </span></p>
</li>
<li>
<p><span>Ready-to-use conversion reagent is added directly to DNA. </span></p>
</li>
<li>
<p><span>High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen Sequencing. </span></p>
</li>
</ul>
</div>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Manual',
			'url' => 'files/products/kits/Auto-Premium-Bisulfite-kit-manual.pdf',
			'slug' => 'auto-premium-bisulfite-kit-manual',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-09-01 12:36:09',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '1176',
			'name' => 'Auto Premium Bisulfite kit SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-US-en-1_0.pdf',
			'countries' => 'US',
			'modified' => '2021-02-09 11:00:57',
			'created' => '2021-02-09 11:00:57',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1174',
			'name' => 'Auto Premium Bisulfite kit SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-GB-en-1_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-02-09 11:00:18',
			'created' => '2021-02-09 11:00:18',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1172',
			'name' => 'Auto Premium Bisulfite kit SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-ES-es-1_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-02-09 10:59:34',
			'created' => '2021-02-09 10:59:34',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1171',
			'name' => 'Auto Premium Bisulfite kit SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-DE-de-1_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-02-09 10:59:13',
			'created' => '2021-02-09 10:59:13',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1175',
			'name' => 'Auto Premium Bisulfite kit SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-JP-ja-1_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-02-09 11:00:39',
			'created' => '2021-02-09 11:00:39',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '1170',
			'name' => 'Auto Premium Bisulfite kit SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-BE-nl-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-02-09 10:58:51',
			'created' => '2021-02-09 10:58:51',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '1169',
			'name' => 'Auto Premium Bisulfite kit SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-BE-fr-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-02-09 10:58:33',
			'created' => '2021-02-09 10:58:33',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '1173',
			'name' => 'Auto Premium Bisulfite kit SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-FR-fr-1_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-02-09 10:59:55',
			'created' => '2021-02-09 10:59:55',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-ipure-kit-v2-x100-100-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C03010010</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1899" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1899" id="CartAdd/1899Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1899" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto IPure kit v2</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto IPure kit v2',
                    'C03010010',
                     '410',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto IPure kit v2',
                    'C03010010',
                    '410',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-ipure-kit-v2-x100-100-rxns" data-reveal-id="cartModal-1899" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto IPure kit v2</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02010034</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1885" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1885" id="CartAdd/1885Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1885" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto hMeDIP kit x16 (monoclonal mouse antibody)</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010034',
                     '690',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010034',
                    '690',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1885" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto hMeDIP kit x16 (monoclonal mouse antibody)</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020011</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1888" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1888" id="CartAdd/1888Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1888" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto MethylCap kit</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                     '695',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                    '695',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-methylcap-kit-x48-48-rxns" data-reveal-id="cartModal-1888" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto MethylCap kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x24-24-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030032</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (24 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x96-96-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030033</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (96 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '1895',
	'antibody_id' => null,
	'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
	'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
	'label1' => 'Characteristics',
	'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label2' => 'NATURE METHODS',
	'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'label3' => '',
	'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
	'format' => '96 rxns',
	'catalog_number' => 'C02030033',
	'old_catalog_number' => '',
	'sf_code' => 'C02030033-',
	'type' => 'RFR',
	'search_order' => '04-undefined',
	'price_EUR' => '4415',
	'price_USD' => '4385',
	'price_GBP' => '4085',
	'price_JPY' => '769000',
	'price_CNY' => '',
	'price_AUD' => '10965',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => true,
	'featured' => true,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'premium-rrbs-kit-x96-96-rxns',
	'meta_title' => 'Premium RRBS Kit | Diagenode ',
	'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
	'meta_description' => 'Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries to efficiently analyze DNA methylation at the single nucleotide level. More cost effective than Whole Genome Bisulfite Sequencing (WGBS) and with greater coverage than microarray-based methods.',
	'modified' => '2022-12-05 11:22:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsRelated' => array(
		'id' => '3234',
		'product_id' => '1893',
		'related_id' => '1895'
	),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	)
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '101',
	'name' => 'Auto Premium Bisulfite kit',
	'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<ul>
<li>
<p><span>Fastest method for complete bisulfite conversion of DNA for methylation analysis. </span></p>
</li>
<li>
<p><span>Ready-to-use conversion reagent is added directly to DNA. </span></p>
</li>
<li>
<p><span>High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen Sequencing. </span></p>
</li>
</ul>
</div>
</div>
</div>
</div>',
	'image_id' => null,
	'type' => 'Manual',
	'url' => 'files/products/kits/Auto-Premium-Bisulfite-kit-manual.pdf',
	'slug' => 'auto-premium-bisulfite-kit-manual',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2015-09-01 12:36:09',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '177',
		'product_id' => '1893',
		'document_id' => '101'
	)
)
$sds = array(
	'id' => '1173',
	'name' => 'Auto Premium Bisulfite kit SDS FR fr',
	'language' => 'fr',
	'url' => 'files/SDS/Bisulfite/SDS-C02030031-Auto_Premium_Bisulfite_kit-FR-fr-1_0.pdf',
	'countries' => 'FR',
	'modified' => '2021-02-09 10:59:55',
	'created' => '2021-02-09 10:59:55',
	'ProductsSafetySheet' => array(
		'id' => '2051',
		'product_id' => '1893',
		'safety_sheet_id' => '1173'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Contact Information

First name

Last name

Company

Phone number

City

Country

State

Email verification^*

Comment

Tweets by Diagenode

Events

APHL 2024
Milwaukee, Wisconsin, USA
May 6-May 9, 2024
London Calling 2024
London, UK
May 21-May 24, 2024

See all events

News

See all news

The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.

Auto Premium Bisulfite kit SDS US en	Download
Auto Premium Bisulfite kit SDS GB en	Download
Auto Premium Bisulfite kit SDS ES es	Download
Auto Premium Bisulfite kit SDS DE de	Download
Auto Premium Bisulfite kit SDS JP ja	Download
Auto Premium Bisulfite kit SDS BE nl	Download
Auto Premium Bisulfite kit SDS BE fr	Download
Auto Premium Bisulfite kit SDS FR fr	Download

Auto Premium Bisulfite kit

How to properly cite this product in your work

Auto IPure kit v2

Auto hMeDIP kit x16 (monoclonal mouse antibody)

Auto MethylCap kit

Premium RRBS kit (24 rxns) - replaced by Premiu...

Premium RRBS kit (96 rxns) - replaced by Premiu...

Events

News