Diagenode

Applications

  • RNA-seq

    Learn more

    Messenger RNA (mRNA)

    Protein-coding RNA with specific poly A selectionCancer-related mRNA signaturesAberrant mRNA translation in cancer pathogenesisGene expression profiling and identification of differentially expressed genes (DEG)Epigenetic mechanism of gene expression (transcriptome plus H3K36me3 and H3K4me3 marks)Functional annotati... Learn more

    Small RNA and miRNA

    Post-transcriptional regulation of gene expressionNovel transcripts and differentially-expressed small RNAsmiRNAs involved in regulation of onco- and tumor-suppressor gene expressionDifferential miRNA expression Learn more

    Whole transcriptome analysis and long non-coding RNA (lncRNA) analysis

    Transcriptome sequencing: Analysis of whole transcriptome and RNAs larger than 200nt with optional rRNA depletionNon-protein coding RNAs involved in chromatin remodeling & transcriptional/post-transcriptional regulation Learn more

  • Services

    Learn more

    Custom NGS services

    Low input libraries from picogramsSpecific analysesComplete customized projects Learn more

    Histone ChIP-seq services

    Use our services to explore ChIP-seq histone modifications down to 10,000 cellsPost-translational modification of histones regulates gene expression. With our Epigenomic Profiling Services, study regulatory elements and their interacting proteins with active promoter and enhancer analysis. Profile genome-wide histon... Learn more

    Transcription factor ChIP-seq services

    Explore the effects of transcription factor binding through ChIP-seq analysis of a multitude of TFs including:CTCF: transcriptional repressor and insulator activityp300/CBP: histone acetyltransferasePol II, p53, and moreEpigenetic writers, readers, erasersNuclear receptorsTumor supressor genes Learn more

  • エピジェネティクス・DNAメチル化

    DNAメチル化研究のための完全打開策 Diagenodeは、DNAメチル化の分野の経験者、未経験者どちらの方でも、実験の一貫性のあるデータを確保しながら、できるだけシンプルかつ便利な実験にするために必要なすべてをご提供いたします。Diagenodeは、超音波装置、試薬キット、高品質の抗体、ハイスループットの自動化機能を備えており、特定のDNAメチル化解析のすべての要件に対応します。 DNAメチル化は最初に発見されたエピジェネティックマークであり、エピジェネティックスにおいて最も広く研究されているトピックです。生体内でDNAは複製後にメチル化され、刷り込みされた遺伝子、X染色体不活性化および癌細胞における腫瘍抑制遺伝子サイレンシングの調節を含む多くの生物学的過程に関与します。.メチル化は、多くの場合プロモーター領域の上流にあるDNAのシトシン - グアニンが豊富な領域(CpGアイランド)で発生します。 参照 エピジェネティック規制でDNAの唯一の変形として、5-メチルシトシン(5-mC)が最も有名です。 しかし2009年に、Kriaucionisは第2のメチル化シトシン、5-ヒドロキシメチルシトシン(5-hmC)を発見しました。いわゆる6番目の塩基は、オキシゲナーゼのTET類による5-メチルシトシン(5-mC)の5-ヒドロキシメチルシトシンへの酵素的変換によって生成されます。初期の報告では、DNAを脱メチル化して5... Learn more

    Bisulfite conversion

    Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine D... Learn more

    Methylated DNA immunoprecipitation

    The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is pr... Learn more

    Methylbinding domain protein

    The MBD approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. The H6-GST-MBD fusion protein can be used to... Learn more

  • エピジェネティクス・クロマチン解析

    エピジェネティクス研究は、異なる転写パターン、遺伝子発現およびサイレンシングを引き起こすクロマチンの変化に対処します。クロマチンの主成分はDNAおよびヒストン蛋白質です。 各ヒストンコア蛋白質(H2A、H2B、H3およびH4)の2つのコピーを8量体に組み込み、DNAで包んでヌクレオソームコアを形成させます。 ヌクレオソームは、転写機械のDNAへの接近可能性およびクロマチン再構成因子を制御します。 クロマチン免疫沈降(ChIP)は、関心対象の特定の蛋白質に対するゲノム結合部位の位置を解明するために使用される方法であり、遺伝子発現の制御に関する非常に貴重な洞察を提供します。 ChIPは特定の抗原を含むクロマチン断片の選択的富化に関与します。 特定の蛋白質または蛋白質修飾を認識する抗体を使用して、特定の遺伝子座における抗原の相対存在量を決定します。 ChIP-seqおよびChIP-qPCRは、蛋白質-DNA結合部位の同定を可能にする技術です。   Learn more

    ChIP-qPCR

    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus o... Learn more

    ChIP-seq

    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life s... Learn more

  • クロマチン断片

    ChIPの成功に最も重要なステップは細胞の固定と溶解、そしてクロマチンの断片化の両方です。 研究者の中には、これらのステップの両方の重要な性質を見過すことがあります。断片化工程の不一致を解消するDiagenodeのPicoruptor®は、最先端の超音波ACT(Adaptive Cavitation Technology)を駆使し、効率的にクロマチンを断片します。ACTは、穏やかかつ非常に有効な断片力を用いて、ChIP実験に必須の高いIP効率および感度を持つ最高のクロマチン品質を可能にします。 さらに、Picoruptor®は、タンパク質-DNA複合体が高感度で偏りのない正確なChIPのために十分に保存されるよう、熱分解からクロマチン断片を保存する正確に制御された温度環境を提供します。 Diagenode Picoruptor®は、qPCRやChIP-seqなど、最適に断片化された偏りのないクロマチンが必須な多くのアプリケーションにぴったりのクロマチン断片を実現します。 Panel A, 10 µl volume: Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes ... Learn more

  • 次世代シーケンシング

    DNA Shearing, library preparation, and automation: your one-stop shop for NGS 1. Choose your shearing device: Shear DNA anywhere from 150 bp to 75 kb Shear down to 5 μl: 150 bp - 2 kb Perfect for NGS DNA library prep and FFPE nucleic acid extraction Shear anywhere from 2 kb – 75 kb Perfect for mate pair library prep and long fragment DNA sequencing Shear 20 or 50 µl with this light desktop device 2. Choose your optimized library preparation kit 3. Choose library prep automation for unmatched data reproducibility Low input of 50 pg... Learn more

    DNA/RNA library preparation

    Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to fragmented DNA or RNA prior to sequencing After input DNA has been fragmented, it is end-repaired and blunt-ended. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphoryl... Learn more

    DNA/RNA shearing

    In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to... Learn more

    FFPE DNA extraction

    Diagenode's high yields FFPE DNA extraction using Bioruptor® is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no toxic reagents, digest tissues, and purify DNA with high y... Learn more

    RNA extraction

    Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. Diageno... Learn more

  • タンパク質分析

    Proteins play a critical role in virtually all cell processes including metabolism, structure, growth, and repair. Understanding protein function, behavior, structure, and their importance are key aspects of understanding disease and therapeutics. Experimental analysis of proteins typically involves expression and purification or the direct extraction of proteins from cells or tissues for further downstream analysis such as ligand binding assays, mass spectrometry, or protein sequencing. Other protein analysis methods include detection methods such as BCA assays and Western blots or methods to understand protein-to-protein in... Learn more

    Protein extraction

    Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purificati... Learn more

    Western blot analysis

    We have what you need for a successful Western blot! Western blots require multiple steps - sample preparation, sample loading, electrophoresis, protein transfer, antibody incubation, and signal detection - so it is important to be sure that each step is done correctly. For a successful Western blot we offer: Loa... Learn more

  • Antibody Applications

    Learn more

    Protein array

    Protein array Learn more

    DB

    Dot blotting Learn more

    ELISA

    Enzyme-linked immunosorbent assay. Learn more

    GSA

    Gel Supershift Learn more

    IC

    Immunochemistry Learn more

    IF

    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°... Learn more

    IHC

    Immunohistochemistry Learn more

    IP

    Immunoprecipitation Learn more

    Peptide array

    Peptide array Learn more

    siRNA Knockdown

    Epigenetic antibodies you can trust! Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-hist... Learn more

    WB

    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Loading control, MW marker visualization. Check our selection of antibod... Learn more

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics