Diagenode

Testimonials

When performing FAIRE-PCR (Formaldehyde-Assisted-Isolated-Regulatory-Elements) experiments, instead of using ethanol precipitation after the phenol-chloroform extraction, we used the Ipure kit to decrosslink and purify DNA. The yield was much better than after ethanol precipitation. In addition, we get rid of problem of inhibition of the following PCR reactions. 

Dr Emmanuèle Mouchel-Vielh, CNRS-UPMC Laboratoire de Biologie du Développement, Paris, France

The Diagenode Plant ChIP-seq kit works very well. The kit gives higher enrichment over background for the positive samples compared to our previous method. Using both the Plant ChIP-seq Kit and Diagenode’s Premium H3K9/14 ac polyclonal antibody, we performed ChIP-qPCR of H3K9/14 ac in region 3 of the TOC1 promoter. TOC1 is a circadian clock gene involved in evening loop that inhibits circadian clock genes expressed during the light phase of the day. We observed higher acetylation around TSS of TOC1 at the end of light phase in short day conditions, results that correlate with previously published data.

Dorota Komar, Centre for Plant Biotechnology and Genomics, Madrid, Spain
about H3K9/14ac polyclonal antibody - Premium, H3K9/14ac polyclonal antibody - Premium (sample size).

The Diagenode MicroPlex kit is an efficient way to make sequencing libraries, especially from samples with very low inputs. We used it for successfully processing ChIP material of transcription factors and histone modifications.

Dr. Filippo Cernilogar (Ludwig-Maximilian-Universität München, Germany)
about MicroPlex Library Preparation Kit v2 (48 indexes), MicroPlex Library Preparation Kit v2 (12 indexes).

When doing CRISPR experiments, it is essential to verify the expression levels of the Cas9 to ensure high-quality results. Diagenode's new CRISPR/Cas9 4G10 antibody is extremely specific, able to recognize different Cas9 fusion proteins (even those used for CRISPRi experiments) and compatible with many biochemical assays, such as immunofluorescence, western blot and immunoprecipitation. Among the different antibodies in the market, Diagenode's 4G10 CRISPR/Cas9 is our antibody of choice.

Researcher at EPFL, Lausanne, Switzerland
about CRISPR/Cas9 monoclonal antibody 4G10 (sample size), CRISPR/Cas9 monoclonal antibody 4G10, CRISPR/Cas9 monoclonal antibody 4G10.

The Diagenode MicroPlex kit is the quickest and most efficient way to make sequencing libraries, especially from samples with very low inputs. We regularly start with picogram amounts of ChIP material and produce excellent quality libraries that would be impossible to make using normal methods. Sequencing libraries made from the MicroPlex kit give us excellent results even in large genomes. The kit performs very well, and we will use the kit in the future for studies with low cell numbers or starting material.

Dr. Morgan Sammons, Lab of Dr. Shelley Berger, University of Pennsylvania
about MicroPlex Library Preparation Kit v2 (12 indexes), MicroPlex Library Preparation Kit v2 (12 indexes), MicroPlex Library Preparation Kit v2 (48 indexes).

Not only does the IP-Star eliminate the problem of human variation associated with producing our samples, it also enables us to produce 1000-2000 ChIP-seq samples per year very reliably. The IP-Star reduces our processing time down from one day of manual work to just one overnight run with only 30 minutes of hands-on work. The IP-Star has made all our ChIPs consistent and the process completely reliable regardless of the operator or the time of day

Dr. John Lambourne, Postdoctorate Researcher at the Innovation Centre, McGill University, Canada
about SX-8G IP-Star® Compact Automated System.

We are very happy with the SX-8G IP-Star that we are routinely using in combination with MeDIP or MethylCap for DNA methylation profiling or for ChIP of histones or transcription factors. The use of the robot significantly reduces the hands-on time, and even more importantly provides a very high reproducibility, which is very valuable and essential for the generation of large number numbers of precious samples that will be analyzed on NGS platforms

Henk Stunnenberg, The Radboud University Nijmegen Medical Centre
about SX-8G IP-Star® Compact Automated System.

We found that the IP-Star® provides an efficient, reliable and accurate tool for the construction of Illumina next-generation RNA-seq libraries, especially for trancriptome-based annotation of larger genomes or genomes with many alternative gene isoforms. The automated protocol significantly saves on-hands experimentation time, related costs and error-prone manual steps. Added benefits include ease of operation and generation of consistent data regardless of human variability and experimental run. Adaptation of this technology should support the unveiling of the mechanisms governing differential gene expression and transcription processing genome-wide, leading to a better understanding of genetic and epigenetic regulation and inheritance in a time-efficient manner.

UB Genomics and Bioinformatics Core, State University of New York at Buffalo, Research Team: Dr N. Nowak, Dr M.J. Buck, Dr M. Tsompana, S. Valiyaparambil, J. Bard, and B. Marzullo.
about SX-8G IP-Star® Compact Automated System.

Automated ChIP experiments in the IP-Star® Compact Automated System can be used to detect changes in transcription factor binding after treatment with small interference RNAs. Compared to conventionnal ChIP, the use of the SX-8G IP-Star® Compact in combination with the Auto ChIP kit saves working time and improves the reproducibility of ChIP experiments

S. Prall, Northern Institute of Cancer Research, Newcastle University
about SX-8G IP-Star® Compact Automated System.

I was using Diagenode Plant ChIP-seq kit to investigate abundance of particular histone mark in A.thaliana's seedlings. Plant ChIP-seq kit yielded in high amounts of immunoprecipitation DNA, very good relative enrichment to input and low background level. I frankly recommend it for ChIP on Plant tissues.

Msc Pawel Mikulski, Lab of Dr. Daniel Schubert, Heinrich Heine University Düsseldorf

The ability to produce libraries starting with low input DNA is primordial when using ChIP-seq. I am a ChIP-seq user and I always use the MicroPlex kit for library making from my immunoprecipitated DNA. Not only it’s quick and requires as little as 50 pg of input DNA but it also never fails. I’ve made over 100 libraries using the MicroPlex library preparation kit, and none of them failed. I use the Microplex kit for preparing libraries from immunoprecipitated DNA and from genomic DNA with low concentration.
As part of a genomics lab, I regularly prepare libraries from a large number of samples. The IP-star® is extremely useful for high throughput library preparation. I have prepared 200 genomic libraries in less than 1 month using the IP-star®, it has helped me economize time and effort and increase reproducibility. I have used the IP-star® for automation of ChIP as well; 16 samples can be processed in one run. In brief, it’s a blessing to have this machine if you apply ChIP-seq to a large number of samples.

Zineb Rchiad, Microbial Genomics Laboratory, King Abdullah University of Science and Technology, Kingdom of Saudi Arabia.
about SX-8G IP-Star® Compact Automated System.

The Megaruptor® shows a more dense size distribution of sheared fragments, and a higher and more reproducible yield.

Sylke Winkler, Sylvia Clausing, Nicola Gscheidel, Yannick Duport, Eugene Myers and Andreas Dahl, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany, and Deep Sequencing Group, BIOTEC, TU Dresden, Germany
about Megaruptor®.

The antibody worked very well, even during our first attempt. We did a 1 hour incubation on a shaker at 4 degrees instead of overnight. We wanted to verify expression of Cas9 in various cell lines I had made (immunofluorescence). We have used the antibody a number of times and it works every time.

Researcher at Harvard University
about CRISPR/Cas9 monoclonal antibody 7A9, CRISPR/Cas9 monoclonal antibody 7A9 (sample size), CRISPR/Cas9 monoclonal antibody 7A9.

The Bioruptor® NGS* is a robust user-friendly sonication system that is key for both reproducible chromatin shearing and DNA shearing. The Bioruptor® NGS consistently delivered optimal DNA shearing that was critical in generating homogeneous sequencing libraries for our experiments. We also observed excellent chromatin shearing result that were crucial for our epigenetic mapping projects.

Dr. Sascha Tierling, Department of Genetics and Epigenetics, Prof . Dr. Jörn Walter's lab, Universität des Saarlandes, Germany.
about Picoruptor ® Sonicator.

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