Diagenode

Infinium Methylation EPIC array service

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Catalog Number
Format
G02090000

The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allows to quantitatively detect the methylation level of over 850,000 human CpG positions throughout the genome with single nucleotide resolution.  

Briefly, upon treatment with bisulfite, unmethylated cytosine bases are converted to uracil, while methylated cytosine bases remain unchanged. Infinium HD array technology interrogates these differentiated loci using site-specific probes (designed for the methylated and unmethylated sites respectively). The level of methylation for the interrogated locus can be determined by calculating the ratio of the fluorescent signals from the methylated vs. unmethylated sites.

Excellent method for comprehensive genome-wide coverage

  • Cost-effective with rapid turnaround time
  • Quantitative analysis of methylation sites in CpG, non-CpG, and CHH sites
  • Over 850,000 human CpG positions at single nucleotide resolution
  • Differentially methylated site analysis
  • Comprehensive  services includes end-to-end bisulfite conversion, array hybridization, and analysis

  • Description

    Infinium MethylationEPIC Array services include:

    QC of the genomic DNA

    • Measurement of DNA concentration

    End-to-end MethylationEPIC Array

    • Bisulfite conversion
    • QC of bisulfite conversion by Methylation-specific PCR (MSP) on specific regions  
    • Whole genome amplification  
    • Array hybridization
    • Single base extension  
    • Array scanner

  • Bioinformatic analysis

    Standard files provided:

    • Sample annotation
    • Variable annotation  
    • Raw data (un-normalized β values and detection p-values)
    • Scanner output

    Additional analysis on request:  

    Differential methylation analysis:

    Identification of differentially methylated CpG between sample groups

    Provided files:

    • Report with summary of differential methylation analysis and plots
    • Files containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea) 
    • File containing differential methylated region (DMRs)

    Gene ontology terms analysis:

    Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in methylated CpGs may indicate the underlying biological processes involved.

    Pathway analysis:

    Identify biochemical pathways in which genes associated with methylated CpG may be overrepresented

  •  Publications

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