The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allows to quantitatively detect the total methylation level of over 850,000 CpG positions throughout the human genome at single nucleotide resolution.
Briefly, upon bisulfite treatment, unmethylated cytosines (both 5mC and 5hmC) are converted to uracils, while methylated cytosines remain unchanged. Infinium HD array technology interrogates these differentiated loci using site-specific probes (designed for the methylated and unmethylated sites respectively). The level of total methylation for the interrogated locus can be determined by calculating the ratio of the fluorescent signals from the methylated vs. unmethylated sites.
Comprehensive method for genome-wide coverage
Cost-effective solution with rapid turnaround time
Over 850,000 CpGs detected in human samples at single nucleotide resolution
Quantitative interrogation of CpG, non-CpG, and CHH sites