The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allows to quantitatively detect the total methylation level of over 850,000 CpG positions throughout the human genome at single nucleotide resolution.
Briefly, upon bisulfite treatment, unmethylated cytosines (both 5mC and 5hmC) are converted to uracils, while methylated cytosines remain unchanged. Infinium HD array technology interrogates these differentiated loci using site-specific probes (designed for the methylated and unmethylated sites respectively). The level of total methylation for the interrogated locus can be determined by calculating the ratio of the fluorescent signals from the methylated vs. unmethylated sites.
Comprehensive method for genome-wide coverage
Cost-effective solution with rapid turnaround time
Over 850,000 CpGs detected in human samples at single nucleotide resolution
Quantitative interrogation of CpG, non-CpG, and CHH sites
Raw data (un-normalized β values and detection p-values)
Scanner output
Differential methylation analysis
Identification of differentially methylated CpGs between sample groups.
Files provided:
Report with summary of differential methylation analysis and plots
File containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea)
File containing differential methylated regions (DMRs)
Gene ontology terms analysis
Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.
Pathway analysis
Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.
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