Infinium MethylationEPIC Array Service

The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina^® technology. It allows to quantitatively detect the total methylation level of over 850,000 methylations sites throughout the human genome at single nucleotide resolution. It offers comprehensive, expert-selected coverage of CpG islands, enhancer regions, open chromatin sites and other important regions of the methylome.

Briefly, upon bisulfite treatment, unmethylated cytosines (both 5mC and 5hmC) are converted to uracils, while methylated cytosines remain unchanged. Infinium HD array technology interrogates these differentiated loci using site-specific probes (designed for the methylated and unmethylated sites respectively). The level of total methylation for the interrogated locus can be determined by calculating the ratio of the fluorescent signals from the methylated vs. unmethylated sites.

Comprehensive Genome-Wide Coverage

• Cost-effective solution with rapid turnaround time
• Over 850,000 CpGs detected in human samples at single nucleotide resolution
• Quantitative interrogation of CpG, non-CpG, and CHH sites
• Differentially methylated site analysis using bioinformatic tools
• Available for 5hmC profiling
• End-to-end services include bisulfite conversion, array hybridization, and analysis

See our other DNA Methylation Profiling Services