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Infinium MethylationEPIC Array Service

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Catalog Number
Format
G02090000

The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allows to quantitatively detect the total methylation level of over 850,000 CpG positions throughout the human genome at single nucleotide resolution.  

Briefly, upon bisulfite treatment, unmethylated cytosines (both 5mC and 5hmC) are converted to uracils, while methylated cytosines remain unchanged. Infinium HD array technology interrogates these differentiated loci using site-specific probes (designed for the methylated and unmethylated sites respectively). The level of total methylation for the interrogated locus can be determined by calculating the ratio of the fluorescent signals from the methylated vs. unmethylated sites.

See our Infinium MethylationEPIC and Hydroxymethylation oxEPIC Array Service if interested in both true methylation (5mC) and hydroxymethylation (5hmC) levels in the DNA sample

Excellent method for comprehensive genome-wide coverage

  • Cost-effective with rapid turnaround time
  • Over 850,000 CpGs detected in human samples at single nucleotide resolution
  • Quantitative interrogation of CpG, non-CpG, and CHH sites
  • Differentially methylated site analysis
  • Comprehensive services include end-to-end bisulfite conversion, array hybridization, and analysis

See our other DNA methylation prodfiling services for reduced representation, whole genome, and targeted analysis

  • Description

    End-to-end Array

    • Bisulfite conversion
    • QC of bisulfite conversion by Methylation-specific PCR (MSP) on 3 specific regions with known methylation status  
    • Whole genome amplification  
    • Array hybridization
    • Single base extension  
    • Array scanner 
  • Bioinformatic analysis

    Analysis

    Features

    Standard

    Standard files provided:

    • Sample annotation
    • Variable annotation
    • Raw data (un-normalized β values and detection p-values)
    • Scanner output

    Differential methylation analysis

    Identification of differentially methylated CpGs between sample groups.

    Files provided:

    • Report with summary of differential methylation analysis and plots
    • File containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea)
    • File containing differential methylated regions (DMRs)

    Gene ontology terms analysis

    Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.

    Pathway analysis

    Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Infinium MethylationEPIC Array Service (Diagenode Cat# G02090000). Click here to copy to clipboard.

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  •  Related products
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      G02090004
      Infinium MethylationEPIC and Hydroxymethylation...

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