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Infinium Methylation EPIC Array受託サービス

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  •  詳細
  •  資料
  •  関連記事
  •   お見積り
カタログ番号
フォーマット
G02090000

Infinium MethylationEPIC Arrayは、バイサルファイト処理とIllumina®技術に基づくゲノムワイドのDNAメチル化解析技術です。これは、一塩基分解能を有するゲノム全体にわたる850,000を超えるヒトCpG位置のメチル化レベルの定量的な検出を可能にします。

バイサルファイトで処理すると、メチル化されたシトシン塩基は変化しませんが、メチル化されていないシトシン塩基はウラシルに変換されます。Infinium HD配列技術は、メチル化部位と非メチル化部位に対して各々に設計された部位特異的プローブを用いて、これらの分化した遺伝子座を調べます。調べられた遺伝子座のメチル化のレベルは、メチル化部位と非メチル化部位との蛍光シグナルの比を計算することによって決定されます。

包括的なゲノムワイドカバレッジのための優れた方法

  • 高いコストパフォーマンス、結果までの時間短縮
  • CpG、非CpG、及びCHH部位におけるメチル化部位の定量解析
  • 一塩基分解能で850,000を超えるヒトCpG位置
  • 異なるメチル化部位解析
  • 徹底したバイサルファイト変換、配列交配、および解析が含まれる包括的なサービス

  • 詳細
    EPIC Array Service

    End-to-end array

    • Bisulfite conversion
    • Whole genome amplification  
    • Array hybridization
    • Single base extension  
    • Array scanner 
  • バイオインフォマティック解析

    Analysis

    Features

    Standard

    Standard files provided:

    • Sample annotation
    • Variable annotation
    • Scanner output (IDAT files)

    Differential methylation analysis

    Identification of differentially methylated CpGs between sample groups.

    Files provided:

    • Report with summary of differential methylation analysis and plots
    • File containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea)
    • File containing differential methylated regions (DMRs)

    Gene ontology terms analysis

    Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.

    Pathway analysis

    Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.

  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Infinium Methylation EPIC Array受託サービス (Diagenode Cat# G02090000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Enhanced cell deconvolution of peripheral blood using DNA methylation for high-resolution immune profiling
    Lucas A Salas, Ze Zhang, Devin C Koestler, Rondi A Butler, Helen M Hansen, Annette M Molinaro, John K Wiencke, Karl T Kelsey, Brock C Christensen
    DNA methylation microarrays can be employed to interrogate cell-type composition in complex tissues. Here, we expand reference-based deconvolution of blood DNA methylation to include 12 leukocyte subtypes (neutrophils, eosinophils, basophils, monocytes, B cells, CD4+ and CD8+ naïve and memory cells, natural kil...

    Interplay between Histone and DNA Methylation Seen through Comparative Methylomes in Rare Mendelian Disorders
    Guillaume Velasco, Damien Ulveling,Sophie Rondeau,Pauline Marzin,Motoko Unoki,Valérie Cormier-Daire, Claire Francastel
    DNA methylation (DNAme) profiling is used to establish specific biomarkers to improve the diagnosis of patients with inherited neurodevelopmental disorders and to guide mutation screening. In the specific case of mendelian disorders of the epigenetic machinery, it also provides the basis to infer mechanistic aspects...

    Genome-wide DNA methylation and transcriptome integration reveal distinct sex differences in skeletal muscle
    Shanie Landen, Macsue Jacques , Danielle Hiam , Javier Alvarez, Nicholas R Harvey, Larisa M. Haupt, Lyn R, Griffiths, Kevin J Ashton, Séverine Lamon, Sarah Voisin, Nir Eynon
    Nearly all human complex traits and diseases exhibit some degree of sex differences, and epigenetics contributes to these differences as DNA methylation shows sex differences in various tissues. However, skeletal muscle epigenetic sex differences remain largely unexplored, yet skeletal muscle displays distinct sex d...

  •  関連商品

イベント

  • DNA methylation workshop: Master MeDIP assay
    Diagenode
    Sep 30, 2021
  • DNA methylation workshop: Become an expert at RRBS
    Diagenode
    Oct 1-Oct 31, 2021
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