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Illumina Infinium MethylationEPIC array BeadChip (850K) service

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  •  描述
  •  文档
  •  相关
  •   获得报价
目录号
格式
G02090000

The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allows to quantitatively detect the methylation level of over 850,000 human CpG positions throughout the genome with single nucleotide resolution.  

Briefly, upon treatment with bisulfite, unmethylated cytosine bases are converted to uracil, while methylated cytosine bases remain unchanged. Infinium HD array technology interrogates these differentiated loci using site-specific probes (designed for the methylated and unmethylated sites respectively). The level of methylation for the interrogated locus can be determined by calculating the ratio of the fluorescent signals from the methylated vs. unmethylated sites.

Excellent method for comprehensive genome-wide coverage

  • Cost-effective with rapid turnaround time
  • Quantitative analysis of methylation sites in CpG, non-CpG, and CHH sites
  • Over 850,000 human CpG positions at single nucleotide resolution
  • Differentially methylated site analysis
  • Comprehensive  services includes end-to-end bisulfite conversion, array hybridization, and analysis

See our other DNA methylation analysis service options for reduced, whole genome, and targeted analysis

  • Description
    EPIC Array Service

    End-to-end array

    • Bisulfite conversion
    • QC of bisulfite conversion by Methylation-specific PCR (MSP) on 3 specific regions with known methylation status  
    • Whole genome amplification  
    • Array hybridization
    • Single base extension  
    • Array scanner 
  • Bioinformatic analysis

    Analysis

    Features

    Standard

    Standard files provided:

    • Sample annotation
    • Variable annotation
    • Raw data (un-normalized β values and detection p-values)
    • Scanner output

    Differential methylation analysis

    Identification of differentially methylated CpGs between sample groups.

    Files provided:

    • Report with summary of differential methylation analysis and plots
    • File containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea)
    • File containing differential methylated regions (DMRs)

    Gene ontology terms analysis

    Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.

    Pathway analysis

    Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.

  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Illumina Infinium MethylationEPIC array BeadChip (850K) service (Diagenode Cat# G02090000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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