The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allows to quantitatively detect the methylation level of over 850,000 human CpG positions throughout the genome with single nucleotide resolution.
Briefly, upon treatment with bisulfite, unmethylated cytosine bases are converted to uracil, while methylated cytosine bases remain unchanged. Infinium HD array technology interrogates these differentiated loci using site-specific probes (designed for the methylated and unmethylated sites respectively). The level of methylation for the interrogated locus can be determined by calculating the ratio of the fluorescent signals from the methylated vs. unmethylated sites.
Excellent method for comprehensive genome-wide coverage
Cost-effective with rapid turnaround time
Quantitative analysis of methylation sites in CpG, non-CpG, and CHH sites
Over 850,000 human CpG positions at single nucleotide resolution
Differentially methylated site analysis
Comprehensive services includes end-to-end bisulfite conversion, array hybridization, and analysis