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Illumina Infinium MethylationEPIC array BeadChip (850K) service

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  •  描述
  •  文档
  •  相关
  •   获得报价
目录号
格式
G02090000

The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allows to quantitatively detect the methylation level of over 850,000 human CpG positions throughout the genome with single nucleotide resolution.  

Briefly, upon treatment with bisulfite, unmethylated cytosine bases are converted to uracil, while methylated cytosine bases remain unchanged. Infinium HD array technology interrogates these differentiated loci using site-specific probes (designed for the methylated and unmethylated sites respectively). The level of methylation for the interrogated locus can be determined by calculating the ratio of the fluorescent signals from the methylated vs. unmethylated sites.

Excellent method for comprehensive genome-wide coverage

  • Cost-effective with rapid turnaround time
  • Quantitative analysis of methylation sites in CpG, non-CpG, and CHH sites
  • Over 850,000 human CpG positions at single nucleotide resolution
  • Differentially methylated site analysis
  • Comprehensive  services includes end-to-end bisulfite conversion, array hybridization, and analysis

See our other DNA methylation analysis service options for reduced, whole genome, and targeted analysis

  • Description
    EPIC Array Service

    End-to-end array

    • Bisulfite conversion
    • Whole genome amplification  
    • Array hybridization
    • Single base extension  
    • Array scanner 
  • Bioinformatic analysis

    Analysis

    Features

    Standard

    Standard files provided:

    • Sample annotation
    • Variable annotation
    • Scanner output (IDAT files)

    Differential methylation analysis

    Identification of differentially methylated CpGs between sample groups.

    Files provided:

    • Report with summary of differential methylation analysis and plots
    • File containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea)
    • File containing differential methylated regions (DMRs)

    Gene ontology terms analysis

    Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.

    Pathway analysis

    Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.

  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Illumina Infinium MethylationEPIC array BeadChip (850K) service (Diagenode Cat# G02090000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Enhanced cell deconvolution of peripheral blood using DNA methylation for high-resolution immune profiling
    Lucas A Salas, Ze Zhang, Devin C Koestler, Rondi A Butler, Helen M Hansen, Annette M Molinaro, John K Wiencke, Karl T Kelsey, Brock C Christensen
    DNA methylation microarrays can be employed to interrogate cell-type composition in complex tissues. Here, we expand reference-based deconvolution of blood DNA methylation to include 12 leukocyte subtypes (neutrophils, eosinophils, basophils, monocytes, B cells, CD4+ and CD8+ naïve and memory cells, natural kil...

    Interplay between Histone and DNA Methylation Seen through Comparative Methylomes in Rare Mendelian Disorders
    Guillaume Velasco, Damien Ulveling,Sophie Rondeau,Pauline Marzin,Motoko Unoki,Valérie Cormier-Daire, Claire Francastel
    DNA methylation (DNAme) profiling is used to establish specific biomarkers to improve the diagnosis of patients with inherited neurodevelopmental disorders and to guide mutation screening. In the specific case of mendelian disorders of the epigenetic machinery, it also provides the basis to infer mechanistic aspects...

    Genome-wide DNA methylation and transcriptome integration reveal distinct sex differences in skeletal muscle
    Shanie Landen, Macsue Jacques , Danielle Hiam , Javier Alvarez, Nicholas R Harvey, Larisa M. Haupt, Lyn R, Griffiths, Kevin J Ashton, Séverine Lamon, Sarah Voisin, Nir Eynon
    Nearly all human complex traits and diseases exhibit some degree of sex differences, and epigenetics contributes to these differences as DNA methylation shows sex differences in various tissues. However, skeletal muscle epigenetic sex differences remain largely unexplored, yet skeletal muscle displays distinct sex d...

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    Nov 12, 2021
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