Polyclonal antibody raised in rabbit against CEBP Alpha (CCAAT/enhancer binding protein (C/EBP), alpha), using a KLH-conjugated synthetic peptide.
Polyclonal antibody raised in rabbit against CEBP Alpha (CCAAT/enhancer binding protein (C/EBP), alpha), using a KLH-conjugated synthetic peptide.
| Lot | 43586 |
|---|---|
| Concentration | 0.59 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 2 |
| Immunoprecipitation | 1:100 - 1:500 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 1 |
* Optimal dilutions/concentrations should be determined by the researcher.
Figure 1. C/EBP alpha antibody IP results
C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell extract A: Control with 3 μg of pre-immune rabbit IgG B: Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (C15410225) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (C15410225) diluted 1:1,000.
Figure 2. C/EBP alpha antibody ChIP results
ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter. Results are expressed as fold enrichment over the IgG negative control.
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