Diagenode

C/EBP alpha polyclonal antibody - Classic

Catalog Number
Format
Price
C15410225-100
100 μl
$335.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against CEBP Alpha (CCAAT/enhancer binding protein (C/EBP), alpha), using a KLH-conjugated synthetic peptide.

Lot40058
Concentration1 μg/μl
Species reactivityHuman, mouse
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 2
Immunoprecipitation 1:100 - 1:500 Fig 1
Western Blotting 1:1,000 Fig 1
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    IP result

    Figure 1. C/EBP alpha antibody IP results
    C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell extract A: Control with 3 μg of pre-immune rabbit IgG B: Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (C15410225) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (C15410225) diluted 1:1,000.

    ChIP result

    Figure 2. C/EBP alpha antibody ChIP results
    ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter. Results are expressed as fold enrichment over the IgG negative control.

  •  Applications
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet CEBP C15410225 DATASHEET
    Datasheet description
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: C/EBP alpha polyclonal antibody - Classic (Diagenode Cat# C15410225-100 Lot# 40058). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  Related products

Events

 See all events

Applications

 See all applications



The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics