C/EBP alpha polyclonal antibody - Classic

100 μl
  Bulk order

Polyclonal antibody raised in rabbit against CEBP Alpha (CCAAT/enhancer binding protein (C/EBP), alpha), using a KLH-conjugated synthetic peptide.

Concentration1 μg/μl
Species reactivityHuman, mouse
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 2
Immunoprecipitation 1:100 - 1:500 Fig 1
Western Blotting 1:1,000 Fig 1
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    IP result

    Figure 1. C/EBP alpha antibody IP results
    C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell extract A: Control with 3 μg of pre-immune rabbit IgG B: Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (C15410225) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (C15410225) diluted 1:1,000.

    ChIP result

    Figure 2. C/EBP alpha antibody ChIP results
    ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter. Results are expressed as fold enrichment over the IgG negative control.

  •  実験手法
    Immunoprecipitation Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet CEBP C15410225 DATASHEET
    Datasheet description
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: C/EBP alpha polyclonal antibody - Classic (Diagenode Cat# C15410225-100 Lot# 40058). Click here to copy to clipboard.

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