Ski3 polyclonal antibody - Classic

Catalog Number
150 µg/50 µl
  Bulk order

Alternative names: TTC37

Polyclonal antibody raised in rabbit against human SKI3, using a recombinant protein.

Concentration3.0 µg/µl
Species reactivityHuman
PurityProtein G purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 Fig 1
Immunofluorescence 1:100 - 1:500 Fig 2, Ref 1
ChIP 1 - 5 μg/ChIP Ref 1
  • Validation Data


    Figure 1. Western blot analysis using the Diagenode antibody directed against SKI3
    Western blot was performed on HeLa nuclear extracts (lane 1) and on the purified human SKI complex (lane 2) (Figure 1A), or on nuclear extracts from HeLa cells (HeLa NE, 20 μg) (Figure 1B) using the Diagenode antibody against SKI3 (Cat. No. pAb-011-150) diluted 1:1,000. The location of the protein is indicated on the right, the marker (in kDa) is shown on the left.


    Figure 2. Immunofluorescence with the Diagenode antibody directed against SKI3
    HeLa cells were stained with the Diagenode antibody against SKI3 (Cat. No. pAb-011-150) (in red), with DAPI to label the DNA (in blue), and with an antibody against α-tubulin to label the cytoplasm (in green). SKI3 is detected in both the cytoplasm and the nucleus but appears to be excluded from the nucleolus.

  • Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
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  • Documents
    Datasheet SKI3 pAb-011-150 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Ski3 polyclonal antibody - Classic (Diagenode Cat# C15410011 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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