Diagenode

Ski3 polyclonal antibody - Classic

カタログ番号
フォーマット
価格
C15410011
(pAb-011-150)
150 µg/50 µl
$315.00
  Bulk order

Alternative names: TTC37

Polyclonal antibody raised in rabbit against human SKI3, using a recombinant protein.

Lot001
Concentration3.0 µg/µl
Species reactivityHuman
TypePolyclonal
PurityProtein G purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 Fig 1
Immunofluorescence 1:100 - 1:500 Fig 2, Ref 1
ChIP 1 - 5 μg/ChIP Ref 1
  • Validation Data

    WB

    Figure 1. Western blot analysis using the Diagenode antibody directed against SKI3
    Western blot was performed on HeLa nuclear extracts (lane 1) and on the purified human SKI complex (lane 2) (Figure 1A), or on nuclear extracts from HeLa cells (HeLa NE, 20 μg) (Figure 1B) using the Diagenode antibody against SKI3 (Cat. No. pAb-011-150) diluted 1:1,000. The location of the protein is indicated on the right, the marker (in kDa) is shown on the left.

    Immunofluorescence

    Figure 2. Immunofluorescence with the Diagenode antibody directed against SKI3
    HeLa cells were stained with the Diagenode antibody against SKI3 (Cat. No. pAb-011-150) (in red), with DAPI to label the DNA (in blue), and with an antibody against α-tubulin to label the cytoplasm (in green). SKI3 is detected in both the cytoplasm and the nucleus but appears to be excluded from the nucleolus.

  •  実験手法
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet SKI3 pAb-011-150 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Ski3 polyclonal antibody - Classic (Diagenode Cat# C15410011 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  関連商品

イベント

  • Genome instability: when RNA meets chromatin
    Roscoff
    Sep 16-Jun 20, 2019
  • Japanese Biochemical Society
    Yokohama
    Sep 18-Sep 20, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    Atlanta, Georgia
    Sep 19, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    RTP, North Carolina
    Sep 20, 2019
 すべてのイベントを見る

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics