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Figure 1. Western blot analysis using the Diagenode antibody directed against SKI3 Western blot was performed on HeLa nuclear extracts (lane 1) and on the purified human SKI complex (lane 2) (Figure 1A), or on nuclear extracts from HeLa cells (HeLa NE, 20 µg) (Figure 1B) using the Diagenode antibody against SKI3 (Cat. No. C15410011) diluted 1:100. The location of the protein is indicated on the right, the marker (in kDa) is shown on the left.
Figure 2. Immunofluorescence with the Diagenode antibody directed against SKI3 HeLa cells were stained with the Diagenode antibody against SKI3 (Cat. No. C15410011) (in red), with DAPI to label the DNA (in blue), and with an antibody against α-tubulin to label the cytoplasm (in green). SKI3 is detected in both the cytoplasm and the nucleus but appears to be excluded from the nucleolus.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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