Ski3 Antibody

Catalog Number
150 µg/50 µl
  Bulk order

Alternative names: TTC37

Polyclonal antibody raised in rabbit against human SKI3, using a recombinant protein.

Concentration3.0 µg/µl
Species reactivityHuman
PurityProtein G purified
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide and 0.05% ProClin 300
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:100 Fig 1
Immunofluorescence 1:100 Fig 2, Ref 1
ChIP 1 - 5 μg/ChIP Ref 1
  • Validation Data

    Ski3 Antibody validated in Western blot

    Figure 1. Western blot analysis using the Diagenode antibody directed against SKI3
    Western blot was performed on HeLa nuclear extracts (lane 1) and on the purified human SKI complex (lane 2) (Figure 1A), or on nuclear extracts from HeLa cells (HeLa NE, 20 µg) (Figure 1B) using the Diagenode antibody against SKI3 (Cat. No. C15410011) diluted 1:100. The location of the protein is indicated on the right, the marker (in kDa) is shown on the left.

    Ski3 Antibody validated in Immunofluorescence

    Figure 2. Immunofluorescence with the Diagenode antibody directed against SKI3
    HeLa cells were stained with the Diagenode antibody against SKI3 (Cat. No. C15410011) (in red), with DAPI to label the DNA (in blue), and with an antibody against α-tubulin to label the cytoplasm (in green). SKI3 is detected in both the cytoplasm and the nucleus but appears to be excluded from the nucleolus.

  • Target Description

    SKI3 is a component of the SKI complex which is composed of SKI2, SKI3 and SKI8. The SKI complex interacts with SKI7, making the link between the SKI complex and the exosome in order to perform mRNA degradation.

  •  Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet SKI3 pAb-011-150 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
    Ski3 Antibody SDS US en Download
    Ski3 Antibody SDS GB en Download
    Ski3 Antibody SDS BE fr Download
    Ski3 Antibody SDS FR fr Download
    Ski3 Antibody SDS ES es Download
    Ski3 Antibody SDS DE de Download
    Ski3 Antibody SDS JP ja Download
    Ski3 Antibody SDS BE nl Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Ski3 Antibody (Diagenode Cat# C15410011 Lot# 001). Click here to copy to clipboard.

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