Diagenode

RING1B polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15430002
100 μg
$325.00
  Bulk order

Polyclonal antibody raised in goat against RING1B (Ring finger protein 1b), using a KLH-conjugated synthetic peptide.

Lot001
Concentration1.0 μg/μl
Species reactivityHuman, chimpanzee, orangutan, mouse, rat, dog, bovine, frog, chicken
TypePolyclonal
PurityAffinity purified
HostGoat
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:5,000 - 1:25,000
Immunofluorescence 1:300 Fig 1
Western Blotting 1:500 - 1:2,000 Fig 2
  • Validation Data

    Immunofluorescence

    Figure 1. RING1B antibody Immunofluorescence results
    Immunofluorescence of RING1B antibody. Tissue: human HeLa cells. Fixation in methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Primary antibody at 1:300 for 20 minutes at 25°C. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.

    Western blot

    Figure 2. RING1B antibody western blot results
    Western blot using the Diagenode RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of RING1B antibody incubated at room temperature.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet RING1B C15430002 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

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