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Figure 1. RING1B antibody Immunofluorescence results Immunofluorescence of RING1B antibody. Tissue: human HeLa cells. Fixation in methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Primary antibody at 1:300 for 20 minutes at 25°C. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.
Figure 2. RING1B antibody western blot results Western blot using the Diagenode RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of RING1B antibody incubated at room temperature.
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
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WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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