Diagenode

H4K8ac polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15310103
(CS-103-100)
100 µl
$295.00
  Bulk order

Polyclonal antibody raised in rabbit against histone H4 containing the acetylated lysine 8 (H4K8ac), using a KLH-conjugated synthetic peptide.

LotA156-001
Concentrationnot determined
Species reactivityHuman
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μl/ChIP Fig 1
ELISA 1:500 Fig 2
Dot Blotting 1:20,000 Fig 3
Western Blotting 1:250 Fig 4

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μl per IP.

  • Validation Data

    ELISA

    Figure 1. Determination of the antibody titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human H4K8ac (cat. No. CS-103-100). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:17,500.

    Dot Blot

    Figure 2. Cross reactivity test using the Diagenode antibody directed against H4K8ac
    A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H4K8ac (cat. No. CS-103-100) with peptides containing other modifications of histone H4 and H3 and an unmodified histone H4 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the modification of interest.

    Western Blot

    Figure 3. Western blot analysis using the Diagenode crude serum against H4K8ac
    Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2c (Cat. No. CS-105-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 120 kDa) is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment of approximately 92 kDa may represent a splicing variant.

    ChIP

    Figure 4. ChIP results obtained with the Diagenode crude serum directed against H4K8ac
    Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode antibody against H4K8ac (cat. No. CS-103-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right. Lane 2 shows the result of the Western analysis with the antibody; lane 1 shows the same analysis after incubation of the antibody with 750 pmol blocking peptide (cat. No. sp-103-050) for 1 hour at room temperature.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    DB
    Dot blotting Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet H4K8ac CS-103-100 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4K8ac polyclonal antibody - Classic (Diagenode Cat# C15310103 Lot# A156-001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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