Diagenode

H3T45p polyclonal antibody - Pioneer

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410321
50 μg /34 μl
$195.00
  Bulk order

Polyclonal antibody raised in rabbit against the region of histone H3 containing the phosphorylated threonine 45 (H3T45p), using a KLH-conjugated synthetic peptide.

LotA2280P
Concentration1.5 µg/µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:5,000 - 1:10,000 Fig 1
Dot Blotting 1:20,000 Fig 2
  • Validation data

    ELISA

    Figure 1. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H3T45p (cat. No. 15410321) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:350,000.

    Dot blot

    Figure 2. Cross reactivity tests using the Diagenode antibody directed against H3T45p
    To test the cross reactivity of the Diagenode antibody against H3T45p (cat. No. 15410321), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3T45. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    DB
    Dot blotting Read more
  • Documents
    Datasheet H3T45p antibody DATASHEET
    Datasheet of H3T45p antibody
    Download
  • Publications

    How to properly cite this product in your work

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