Diagenode

GST monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200207
500 µg
$325.00
  Bulk order

Polyclonal antibody raised in mouse against GST (Glutathione-S-Transferase), using a recombinant protein.

Lot001
Concentration1 μg/μl
Species reactivity
TypeMonoclonal
PurityProtein A purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100,000 - 1:900,000
Western Blotting 1:1,000 - 1:10,000 Fig 1, 2
  • Validation Data

    Western Blot

    Figure 1. GST antibody western blot results
    Western Blot of GST antibody. Lane 1: molecular weight marker. Lane 2: Mouse- anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha- 1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 μg per lane. Primary antibody: Each primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 overnight at 4°C.

    Western Blot

    Figure 2. GST antibody western blot results
    Western Blot of GST antibody. Lane 1: GST recombinant protein. Lane 2: lysate of HeLa cells expressing recombinant GST protein. Load: 0.1 μg per lane. Primary antibody used at 1.0 μg/ml for 1 h at room temperature. Secondary antibody: IRDye800TM goat anti-mouse secondary antibody at 1:5,000 for 45 min at RT.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet GST C15200207 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: GST monoclonal antibody - Classic (Diagenode Cat# C15200207 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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    Sep 27-Sep 30, 2016
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