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Figure 1. GST antibody western blot results Western Blot of GST antibody. Lane 1: molecular weight marker. Lane 2: Mouse- anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha- 1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 μg per lane. Primary antibody: Each primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 overnight at 4°C.
Figure 2. GST antibody western blot results Western Blot of GST antibody. Lane 1: GST recombinant protein. Lane 2: lysate of HeLa cells expressing recombinant GST protein. Load: 0.1 μg per lane. Primary antibody used at 1.0 μg/ml for 1 h at room temperature. Secondary antibody: IRDye800TM goat anti-mouse secondary antibody at 1:5,000 for 45 min at RT.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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