Figure 1. GST antibody western blot results
Western Blot of GST antibody. Lane 1: molecular weight marker. Lane 2: Mouse- anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha- 1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 μg per lane. Primary antibody: Each primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 overnight at 4°C.