H3K18me3 polyclonal antibody

Catalog Number
50 μg
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (trimethyl Lys18), using a KLH-conjugated synthetic peptide.

Concentration0.6 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP 2-5 μg/million cells
Immunofluorescence 1:50 Fig 1
Western Blotting 1:500 Fig 2
Immunochemistry 1:50
Dot Blotting 1:1,000 Fig 3
  • Validation Data


    Figure 1. Immunofluorescence
    Immunofluorescence of H3K18me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K18me3 is nuclear and chromosomal. Staining: Histone H3K18me3 is expressed in green, nuclei are counterstained with DAPI (blue).

    Western Blot

    Figure 2. Western Blot
    Western Blot of H3K18me3 antibody. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Dot Blot

    Figure 3. Dot Blot
    Dot Blot of H3K18me3 antibody. Lane 1: K18 unmodified. Lane 2: K18Me1. Lane 3: K18Me2. Lane 4: K18Me3. Lane 5: K18Ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 0.5 μg/ml for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  •  実験手法
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Dot blotting Read more
    Immunochemistry Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet H3K18me3 C15410292 DATASHEET
    Datasheet description
  •  出版物

    How to properly cite this product in your work

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