TIP5 Antibody

Catalog Number
100 µl
  Bulk order
Other format

Alternative names: BAZ2A, WALp3

Polyclonal antibody raised in rabbit against TIP5 (Transcription termination factor I-interacting protein 5), using the recombinant protein.

Concentrationnot determined
Species reactivityHuman, mouse
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μl/ChIP Fig 1
Western Blotting 1:1,000 Fig 2

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μl per IP.

  • Validation Data

    TIP5 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against TIP5
    ChIP assays were performed using the Diagenode antibody against TIP5 (cat. No. CS-090-100). Chromatin from HEK293T and NIH3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor (Diagenode) to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4°C with 100 μg sheared chromatin and either 5 μl of the TIP5 antibody or 5 μl IgG which was used as negative IP control. The IP’d DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. Figure 1 shows the recovery by the TIP5 antibody and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.

    TIP5 Antibody validated in Western Blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against TIP5
    Western blot was performed on 150 μg nuclear extract from either NIH3T3 or HeLa cells with the Diagenode antibody against TIP5 (cat. No. CS-090-100), diluted 1:1,000 in PBS containing 5% milk powder and 0.1% Tween- 20. The molecular weight marker is shown on the left, the location of the protein of interest is indicated on the right.

  • Target Description

    TIP 5 (UniProt/Swiss-Prot entry Q9UIF9) is the large subunit of the nucleolar remodeling complex NoRC. NoRC causes the repression of ribosomal gene transcription. It was demonstrated that histone deacetylation is involved in this repression and that TIP5 is associated with the histone deacetylase HDAC1 and mediates the deacetylation of histones in the vicinity of the rDNA promoter. The interaction of TIP5 and HDAC1, which is necessary for transcriptional repression, is established by the C-terminal PHD finger and bromodomain.

  •  Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet TIP5 CS-090-100 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
    SDS C15310090 TIP5 Antibody GB en Download
    SDS C15310090 TIP5 Antibody US en Download
    SDS C15310090 TIP5 Antibody BE fr Download
    SDS C15310090 TIP5 Antibody ES es Download
    SDS C15310090 TIP5 Antibody FR fr Download
    SDS C15310090 TIP5 Antibody DE de Download
    SDS C15310090 TIP5 Antibody BE nl Download
    SDS C15310090 TIP5 Antibody JP ja Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: TIP5 Antibody (Diagenode Cat# C15310090 Lot# A300-004 ). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The RNA helicase DHX9 establishes nucleolar heterochromatin, and this activity is required for embryonic stem cell differentiation
    Leone S. et al.
    Long non-coding RNAs (lncRNAs) have been implicated in the regulation of chromatin conformation and epigenetic patterns. lncRNA expression levels are widely taken as an indicator for functional properties. However, the role of RNA processing in modulating distinct features of the same lncRNA is less understood. The ...

    ING1 regulates rRNA levels by altering nucleolar chromatin structure and mTOR localization
    Rajarajacholan U.K. et al.
    Epigenetic, transcriptional and signaling processes in the nucleolus regulate rRNA transcription and cell growth. We report here that the tumor suppressor ING1b binds rDNA, regulates rDNA chromatin modifications and affects nucleolar localization of mTOR to modulate rRNA levels. ING1 represses rDNA transcription by ...

    BAZ2A (TIP5) is involved in epigenetic alterations in prostate cancer and its overexpression predicts disease recurrence.
    Gu L, Frommel SC, Oakes CC, Simon R, Grupp K, Gerig CY, Bär D, Robinson MD, Baer C, Weiss M, Gu Z, Schapira M, Kuner R, Sültmann H, Provenzano M, , Yaspo ML, Brors B, Korbel J, Schlomm T, Sauter G, Eils R, Plass C, Santoro R
    Prostate cancer is driven by a combination of genetic and/or epigenetic alterations. Epigenetic alterations are frequently observed in all human cancers, yet how aberrant epigenetic signatures are established is poorly understood. Here we show that the gene encoding BAZ2A (TIP5), a factor previously implicated in ep...

    lncRNA Maturation to Initiate Heterochromatin Formation in the Nucleolus Is Required for Exit from Pluripotency in ESCs.
    Savić N, Bär D, Leone S, Frommel SC, Weber FA, Vollenweider E, Ferrari E, Ziegler U, Kaech A, Shakhova O, Cinelli P, Santoro R
    The open chromatin of embryonic stem cells (ESCs) condenses into repressive heterochromatin as cells exit the pluripotent state. How the 3D genome organization is orchestrated and implicated in pluripotency and lineage specification is not understood. Here, we find that maturation of the long noncoding RNA (lncRNA) ...

    Interaction of nucleolin with ribosomal RNA genes and its role in RNA polymerase I transcription.
    Cong R, Das S, Ugrinova I, Kumar S, Mongelard F, Wong J, Bouvet P
    Nucleolin is a multi-functional nucleolar protein that is required for ribosomal RNA gene (rRNA) transcription in vivo, but the mechanism by which nucleolin modulates RNA polymerase I (RNAPI) transcription is not well understood. Nucleolin depletion results in an increase in the heterochromatin mark H3K9me2 and a de...

    The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats
    Guetg C, Lienemann P, Sirri V, Grummt I, Hernandez-Verdun D, Hottiger MO, Fussenegger M, Santoro R
    Maintenance of specific heterochromatic domains is crucial for genome stability. In eukaryotic cells, a fraction of the tandem-repeated ribosomal RNA (rRNA) genes is organized in the heterochromatic structures. The principal determinant of rDNA silencing is the nucleolar remodelling complex, NoRC, that consists of T...

    Chromatin remodeling by imitation switch (ISWI) class ATP-dependent remodelers is stimulated by histone variant H2A.Z.
    Goldman JA, Garlick JD, Kingston RE
    ATP-dependent chromatin remodeling complexes rearrange nucleosomes by altering the position of DNA around the histone octamer. Although chromatin remodelers and the histone variant H2A.Z colocalize on transcriptional control regions, whether H2A.Z directly affects remodeler association or activity is unclear. We det...

    Epigenetic engineering of ribosomal RNA genes enhances protein production.
    Santoro R, Lienemann P, Fussenegger M
    Selection of mammalian high-producer cell lines remains a major challenge for the biopharmaceutical manufacturing industry. Ribosomal RNA (rRNA) genes encode the major component of the ribosome but many rRNA gene copies are not transcribed due to epigenetic silencing by the nucleolar remodelling complex (NoRC) [6], ...

  •  Related products


  • FASEB Biological Methylation: Fundamental Mechanisms
    Porto, Portugal
    Jul 28-Aug 1, 2024
 See all events


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy