Diagenode

>>>   Click for Diagenode’s approach to COVID-19

TIP5 Antibody - ChIP Grade (sample size)

Catalog Number
Format
Price
C15310090-20
20 µl
$95.00
  Bulk order
Other format



Alternative names: BAZ2A, WALp3

Polyclonal antibody raised in rabbit against TIP5 (Transcription termination factor I-interacting protein 5), using the recombinant protein.

LotA300-004
Concentrationnot determined
Species reactivityHuman, mouse
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μl/ChIP Fig 1
Western Blotting 1:1,000 Fig 2

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μl per IP.

  • Validation Data

    TIP5 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against TIP5
    ChIP assays were performed using the Diagenode antibody against TIP5 (cat. No. CS-090-100). Chromatin from HEK293T and NIH3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor (Diagenode) to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4°C with 100 μg sheared chromatin and either 5 μl of the TIP5 antibody or 5 μl IgG which was used as negative IP control. The IP’d DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. Figure 1 shows the recovery by the TIP5 antibody and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.

    TIP5 Antibody validated in Western Blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against TIP5
    Western blot was performed on 150 μg nuclear extract from either NIH3T3 or HeLa cells with the Diagenode antibody against TIP5 (cat. No. CS-090-100), diluted 1:1,000 in PBS containing 5% milk powder and 0.1% Tween- 20. The molecular weight marker is shown on the left, the location of the protein of interest is indicated on the right.

  • Target Description

    TIP 5 (UniProt/Swiss-Prot entry Q9UIF9) is the large subunit of the nucleolar remodeling complex NoRC. NoRC causes the repression of ribosomal gene transcription. It was demonstrated that histone deacetylation is involved in this repression and that TIP5 is associated with the histone deacetylase HDAC1 and mediates the deacetylation of histones in the vicinity of the rDNA promoter. The interaction of TIP5 and HDAC1, which is necessary for transcriptional repression, is established by the C-terminal PHD finger and bromodomain.

  •  Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Datasheet TIP5 CS-090-100 DATASHEET
    Datasheet description
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: TIP5 Antibody - ChIP Grade (sample size) (Diagenode Cat# C15310090-20 Lot# A300-004 ). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics