Diagenode

S. aureus CRISPR/Cas9 monoclonal antibody

Catalog Number
Format
Price
C15200230-100
100 μg
$500.00
  Bulk order
Other format



Monoclonal antibody raised in mouse against the N-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.

Lot001
Concentration2 μg/μl
Species reactivityMouse
TypeMonoclonal
PurityProtein G purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:4,000 Fig 1
Immunoprecipitation 5 μg/IP Fig 2
Immunofluorescence 1:400 Fig 3
  • Validation data

    WB figure 1

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against S. aureus CRISPR/ Cas9
    Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus CRISPR/Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.

    IP figure 2

    Figure 2. IP using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9
    IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1, 3 and 5), or untransfected cells (lane 2, 4 and 6) using 5 μg of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230). The immunoprecipitated proteins were subsequently analysed by Western blot. The results obtained with the Cas9 antibody are shown in lane 3 and 4. The negative control (IP with beads only) is shown in lane 5 and 6, the input (10 μg) is shown in lane 1 and 2.

    IF figure 3

    Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9
    Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/Cas9 antibody (cat. No. C15200230) diluted 1:400 in blocking solution at 4°C o/n, followed by incubation with an anti-mouse secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.

  •  Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IP
    Immunoprecipitation Read more
  •  Documents
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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    S. aureus CRISPR/Cas9 monoclonal antibody tds DATASHEET
    Datasheet of the S. aureus CRISPR/Cas9 monoclonal antibody
    Download
    Accurate QC to optimize CRISPR/Cas9 genome editing specificity POSTER
    The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease res...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: S. aureus CRISPR/Cas9 monoclonal antibody (Diagenode Cat# C15200230-100 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Life-Long AAV-Mediated CRISPR Genome Editing in Dystrophic Heart Improves Cardiomyopathy without Causing Serious Lesions in mdx Mice.
    Xu L, Lau YS, Gao Y, Li H, Han R
    Previous studies from others and us have demonstrated that CRISPR genome editing could offer a promising therapeutic strategy to restore dystrophin expression and function in the skeletal muscle and heart of Duchenne muscular dystrophy (DMD) mouse models. However, the long-term efficacy and safety of CRISPR genome-e...

    Long-term evaluation of AAV-CRISPR genome editing for Duchenne muscular dystrophy.
    Nelson CE, Wu Y, Gemberling MP, Oliver ML, Waller MA, Bohning JD, Robinson-Hamm JN, Bulaklak K, Castellanos Rivera RM, Collier JH, Asokan A, Gersbach CA
    Duchenne muscular dystrophy (DMD) is a monogenic disorder and a candidate for therapeutic genome editing. There have been several recent reports of genome editing in preclinical models of Duchenne muscular dystrophy, however, the long-term persistence and safety of these genome editing approaches have not been addre...

    CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsufficiency.
    Matharu N, Rattanasopha S, Tamura S, Maliskova L, Wang Y, Bernard A, Hardin A, Eckalbar WL, Vaisse C, Ahituv N
    A wide range of human diseases result from haploinsufficiency, where the function of one of the two gene copies is lost. Here, we targeted the remaining functional copy of a haploinsufficient gene using CRISPR-mediated activation (CRISPRa) in and heterozygous mouse models to rescue their obesity phenotype. Transgeni...

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