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S. aureus CRISPR/Cas9 polyclonal antibody (C-terminal)

Catalog Number
100 μl
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Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.

Concentration100 μl
Species reactivityRabbit
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:10,000 Fig 1
Immunoprecipitation 1 μg/IP Fig 2
Immunofluorescence 1:1,000 Fig 3
  • Validation data

    WB figure 1

    Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)
    Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.

    IP figure 2

    Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)
    IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.

    IF figure 3

    Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)
    Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.

  •  Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunoprecipitation Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  •  Documents
    S. aureus CRISPR/Cas9 polyclonal antibody (C-terminal) tds DATASHEET
    Datasheet of the S. aureus CRISPR/Cas9 polyclonal antibody (C-terminal).
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Accurate QC to optimize CRISPR/Cas9 genome editing specificity POSTER
    The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease res...
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: S. aureus CRISPR/Cas9 polyclonal antibody (C-terminal) (Diagenode Cat# C15310259-100 Lot# A2555-001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors
    Kleinjan D.A. et al.
    The nuclease-deactivated variant of CRISPR-Cas9 proteins (dCas9) fused to heterologous transactivation domains can act as a potent guide RNA sequence-directed inducer or repressor of gene expression in mammalian cells. In such a system the long-term presence of a stable dCas9 effector can be a draw-back precluding t...

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