PHF8 polyclonal antibody - Pioneer

Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PHF8 (Cat. No. pAb-064-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,500.

Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8
E14TG2A mouse embryonic stem cells were transfected with a conditional allele of PHF8. Nuclear extracts (135 μg) from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. pAb-064-050). The antibody was diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.