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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PHF8 (Cat. No. pAb-064-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,500.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br />E14TG2A mouse embryonic stem cells were transfected with a conditional allele of PHF8. Nuclear extracts (135 μg) from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. pAb-064-050). The antibody was diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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Controller::render() - CORE/Cake/Controller/Controller.php, line 963
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Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PHF8 (Cat. No. pAb-064-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,500.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br />E14TG2A mouse embryonic stem cells were transfected with a conditional allele of PHF8. Nuclear extracts (135 μg) from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. pAb-064-050). The antibody was diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><span>Polyclonal antibody raised in rabbit against human PHF8 (PHD finger protein 8), using 3 different KLH-conjugated synthetic peptides.</span></p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410064_fig1.jpg" alt="ELISA" height="285" width="357" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PHF8 (Cat. No. pAb-064-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,500.</small></p>
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<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410064_fig2.jpg" alt="Western blot" height="159" width="285" /></p>
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<div class="small-6 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br />E14TG2A mouse embryonic stem cells were transfected with a conditional allele of PHF8. Nuclear extracts (135 μg) from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. pAb-064-050). The antibody was diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×