Diagenode

PHF8 polyclonal antibody

目录号
格式
价格
C15410064
(pAb-064-050)
50 µg/39 µl
$260.00
  Bulk order



Alternative names: JHDM1F, MRXSSD, ZNF422

Polyclonal antibody raised in rabbit against human PHF8 (PHD finger protein 8), using 3 different KLH-conjugated synthetic peptides.

LotA138-0031
Concentration1.3 µg/µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 - 1:500 Fig 1
Western Blotting 1:1,000 Fig 2
  • Validation Data

    ELISA

    Figure 1. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PHF8 (Cat. No. pAb-064-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,500.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8
    E14TG2A mouse embryonic stem cells were transfected with a conditional allele of PHF8. Nuclear extracts (135 μg) from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. pAb-064-050). The antibody was diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  •  应用
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  文档
    Datasheet PHF8 pAb-064-050 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  •  Safety sheets
    PHF8 Antibody SDS US en Download
    PHF8 Antibody SDS GB en Download
    PHF8 Antibody SDS ES es Download
    PHF8 Antibody SDS DE de Download
    PHF8 Antibody SDS JP ja Download
    PHF8 Antibody SDS BE nl Download
    PHF8 Antibody SDS BE fr Download
    PHF8 Antibody SDS FR fr Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PHF8 polyclonal antibody (Diagenode Cat# C15410064 Lot# A138-0031). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

 


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy