NFKB cRel Antibody

Catalog Number
100 μl
  Bulk order

Polyclonal antibody raised in rabbit against NFKB p50 (NFKB1), using a KLH-conjugated synthetic peptide.

Concentrationnot determined
Species reactivityHuman
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:5,000 - 1:25,000
Gel Shift 1:500
Western Blotting 1:500 - 1:3,000 Fig 1
  • Validation Data

    Western Blot

    Figure 1. NFkB cRel antibody western blot results
    Western blot of HeLa cell extract. All incubations were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5’ each. The western blot was incubated with secondary antibody (HRP Goat anti Rabbit IgG) diluted 1:2,000 for 1 h.

  •  Applications
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
    Datasheet NFKB cRel C15310257 DATASHEET
    Datasheet description
  •  Safety sheets
    SDS C15310257 NFKB cRel Antibody GB en Download
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  •  Publications

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