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Figure 1. NFkB cRel antibody western blot results Western blot of HeLa cell extract. All incubations were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5’ each. The western blot was incubated with secondary antibody (HRP Goat anti Rabbit IgG) diluted 1:2,000 for 1 h.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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