Diagenode

NFKB cRel polyclonal antibody - Classic

カタログ番号
フォーマット
価格
C15310257
100 μl
$345.00
  Bulk order



Polyclonal antibody raised in rabbit against NFKB p50 (NFKB1), using a KLH-conjugated synthetic peptide.

Lot001
Concentrationnot determined
Species reactivityHuman
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:5,000 - 1:25,000
Gel Shift 1:500
Western Blotting 1:500 - 1:3,000 Fig 1
  • Validation Data

    Western Blot

    Figure 1. NFkB cRel antibody western blot results
    Western blot of HeLa cell extract. All incubations were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5’ each. The western blot was incubated with secondary antibody (HRP Goat anti Rabbit IgG) diluted 1:2,000 for 1 h.

  •  実験手法
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  資料
    Datasheet NFKB cRel C15310257 DATASHEET
    Datasheet description
    Download
  •  出版物

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