Diagenode

Megaruptor 3 DNAFluid+ Kit

Catalog Number
Format
Price
E07020001
16 samples
$335.00
Other format

The DNAFluid+ Kit for viscous DNA

The DNAFluid+ Kit for viscous DNA  eliminates the challenges of using highly viscous extracted DNA samples for any downstream steps and QC. The DNAFluid+ Kit consists of proprietary "Hydropore-Syringe" and "Hydro Tube" shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the Megaruptor 3. This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. 


TESTIMONIAL

Circulomics is excited to collaborate with Diagenode to develop rapid and easy methods to facilitate the handling and shearing of HMW DNA. Combining Nanobind HMW DNA with Megaruptor shearing and Short Read Eliminator size selection allows accurate control of DNA size for long read sequencing.

Circulomics

Dr. Kelvin Liu, Founder and CEO of Circulomics Inc
  • Data

    Validation data: DNAFluid+ Kit for viscous DNA

    Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.

    DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.

    Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.

    Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.

    Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.

  • Characteristics

    The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor® 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.

    Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use

    Storage
    Store at room temperature

    Precautions
    This product is for research use only. Not for use in diagnostic or therapeutic procedures

  • Video

  •  Documents
    Megaruptor® 3 FLYER
    Your partner in long-read sequencing Designed to provide simple and automated DNA sample prepara...
    Download
    Megaruptor® 3 Quick guide QUICK GUIDE
    The Megaruptor® 3 is intended for laboratory use. It is not suitable for clinical use. Use of...
    Download
    DNAFluid+ guide PROTOCOL
    This is the protocol for DNAFluid+ Kit (long version) where you will find some more tips and tric...
    Download
    DNAFluid+ Quickguide PROTOCOL
    This is a short version of the DNAFluid+ protocol. For more details, read the full guide.
    Download
    Using the DNAFluid+ Kit for viscous DNA to effectively pre-condition high molecular weight DNA for consistent shearing APPLICATION NOTE
    This application note demonstrates that the PacBio Circulomics SRE XS is a good substitute for ge...
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Megaruptor 3 DNAFluid+ Kit (Diagenode Cat# E07020001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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