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<h1 style="text-align: center;"><strong>PRE-RELEASE PROGRAM</strong></h1>
<h2 style="text-align: center;">Be involved in the optimization of the solution</h2>
<p style="text-align: center;">Handling <strong>highly viscous extracted DNA samples </strong>for any downstream steps and QC should not be a challenge anymore. The purpose is to combine the <strong>DNAFluid+ Kit </strong>with the <strong>Megaruptor 3</strong> in order to allow an effective and automatized homogenization of highly viscous DNA samples by keeping the integrity of the DNA and a full control of the DNA size prior any downstream applications.</p>
<p style="text-align: center;">You can be part of the improvement of the solution while getting an early access to the kit and giving us your comments, concerns, and suggestions.</p>
<h2 style="text-align: center;"><strong>How to participate ?</strong></h2>
<p style="text-align: center;">You can get an early access by ordering the DNAFluid+ kit as for other Diagenode products and feedback us on the product by clicking on the below button.</p>
<p style="text-align: center;"><strong>Share your experience and protocols</strong></p>
<p> </p>
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'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3>
<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<h1 style="text-align: center;"><strong>PRE-RELEASE PROGRAM</strong></h1>
<h2 style="text-align: center;">Be involved in the optimization of the solution</h2>
<p style="text-align: center;">Handling <strong>highly viscous extracted DNA samples </strong>for any downstream steps and QC should not be a challenge anymore. The purpose is to combine the <strong>DNAFluid+ Kit </strong>with the <strong>Megaruptor 3</strong> in order to allow an effective and automatized homogenization of highly viscous DNA samples by keeping the integrity of the DNA and a full control of the DNA size prior any downstream applications.</p>
<p style="text-align: center;">You can be part of the improvement of the solution while getting an early access to the kit and giving us your comments, concerns, and suggestions.</p>
<h2 style="text-align: center;"><strong>How to participate ?</strong></h2>
<p style="text-align: center;">You can get an early access by ordering the DNAFluid+ kit as for other Diagenode products and feedback us on the product by clicking on the below button.</p>
<p style="text-align: center;"><strong>Share your experience and protocols</strong></p>
<p> </p>
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<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
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<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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'description' => '<p>The Megaruptor 3 shearing kitには、すぐに使えるHydropore-SyringeとHydro Tubesの合計16サンプルが含まれています。 Shearing kitはMegaruptor®3（B06010003）と併用するよう特別設計になっており、<strong>5 kbから100 kb以上</strong>の範囲で再現性のあるDNA断片を生成が可能です。</p>
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<div id="info1" class="content active">
<p><strong>Storage</strong><br />Store at room temperature</p>
<p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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'search_order' => '',
'price_EUR' => '370',
'price_USD' => '390',
'price_GBP' => '300',
'price_JPY' => '57960',
'price_CNY' => '',
'price_AUD' => '975',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
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'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'megaruptor-3-shearing-kit',
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'ProductsGroup' => array(
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$edit = ''
$testimonials = ''
$featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p><span>Circulomics is excited to collaborate with Diagenode to develop rapid and easy methods to facilitate the handling and shearing of HMW DNA. Combining Nanobind HMW DNA with Megaruptor shearing and Short Read Eliminator size selection allows accurate control of DNA size for long read sequencing.</span></p>
<div class="small-12 columns"><center><img src="../../img/partners/circulomics.png" /></center></div>
<p class="small" text-align="right"><a href="https://example.com">Circulomics</a></p><cite>Dr. Kelvin Liu, Founder and CEO of Circulomics Inc</cite></blockquote>
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<div class="small-12 columns"><center><img src="../../img/partners/circulomics.png" /></center></div>
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'modified' => '2020-12-10 16:40:33',
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<button class="alert small button expand" onclick="$(this).addToCart('Megaruptor 3 Shearing Kit',
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<div class="small-6 medium-6 large-6 columns">
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<div class="small-12 medium-12 large-12 columns">
<h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Megaruptor<sup>®</sup> 3</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>
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<div class="small-3 large-2 columns">
<span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
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<div class="small-3 large-2 columns">
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<div class="small-12 columns" >
<h6 style="height:60px">Megaruptor® 3</h6>
</div>
</div>
</li>
'
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'id' => '3009',
'antibody_id' => null,
'name' => 'Megaruptor<sup>®</sup> 3',
'description' => '<p><a href="https://go.diagenode.com/l/928883/2023-04-06/3jhlc"><img src="https://www.diagenode.com/img/banners/pacbio-mega-promo-2023.png" /></a></p>
<p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>
<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p>',
'label1' => 'Demonstrated shearing of DNA from 5 -100 kb',
'info1' => '<h3>Excellent reproducibility and size distribution achieved with the Megaruptor 3</h3>
<center><img src="https://www.diagenode.com/img/product/shearing_technologies/mega3-results.png" /></center>
<p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>',
'label2' => 'Specifications for the Megaruptor',
'info2' => '<table style="width: 942px;">
<tbody>
<tr style="height: 55px;">
<th class="text-center" style="width: 296px; height: 55px;"></th>
<th class="text-center" style="width: 307px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" caption="false" width="120" height="83" />Megaruptor 3</th>
<th class="text-center" style="width: 329px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" alt="Megaruptor 2" caption="false" width="109" height="75" />Megaruptor 2</th>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Size range</td>
<td class="text-center" style="width: 307px; height: 38px;">5 - 100 kb</td>
<td class="text-center" style="width: 329px; height: 38px;">3 - 75 kb</td>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Volume range</td>
<td class="text-center" style="width: 307px; height: 38px;">65 - 500 <span>µl</span></td>
<td class="text-center" style="width: 329px; height: 38px;">50 - 400 <span>µl</span></td>
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<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Concentration range</td>
<td class="text-center" style="width: 307px; height: 38px;"><span><span>0 - 150 ng/</span></span><span><span>µl</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span><span>0 - 50 ng/</span></span><span><span>µl</span></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Throughput</td>
<td class="text-center" style="width: 307px; height: 38px;"><span><span>1 to 8 samples in parallel</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span>1 to 2 samples in series</span></td>
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<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Touchscreen</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
</tr>
<tr style="height: 56px;">
<td style="width: 296px; height: 56px;">Eliminate cross-contamination</td>
<td class="text-center" style="width: 307px; height: 56px;">with disposable elements in a completely closed-system</td>
<td class="text-center" style="width: 329px; height: 56px;">with disposable elements and with washing sequences</td>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Multiple-orifice disposables</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
</tr>
<tr style="height: 32px;">
<td style="width: 296px; height: 32px;">Consumables</td>
<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
<td class="text-center" style="width: 329px; height: 32px; text-align: center;">
<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
</td>
</tr>
</tbody>
</table>',
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'format' => '1 unit',
'catalog_number' => 'B06010003',
'old_catalog_number' => '',
'sf_code' => 'B06010003-',
'type' => 'DIS',
'search_order' => '',
'price_EUR' => '27500',
'price_USD' => '31500',
'price_GBP' => '24500',
'price_JPY' => '4307875',
'price_CNY' => '0',
'price_AUD' => '78750',
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<h1 style="text-align: center;"><strong>PRE-RELEASE PROGRAM</strong></h1>
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<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<td class="text-center" style="width: 307px; height: 56px;">with disposable elements in a completely closed-system</td>
<td class="text-center" style="width: 329px; height: 56px;">with disposable elements and with washing sequences</td>
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<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
<td class="text-center" style="width: 329px; height: 32px; text-align: center;">
<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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<h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Megaruptor<sup>®</sup> 3</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>
</div>
</div>
<form action="/jp/quotes/quote?id=3009" id="Quote-3009" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="3009" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="eDVvNkRPMHZ6dkRLbUpMaTZubER5dz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="megaruptor-3" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/>
<div class="row collapse">
<h2>Contact Information</h2>
<div class="small-3 large-2 columns">
<span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Phone number</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">City</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-3009">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d'Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People's Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People's Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
<option value="LU">Luxembourg</option>
<option value="MO">Macao</option>
<option value="MK">Macedonia, Republic of</option>
<option value="MG">Madagascar</option>
<option value="MW">Malawi</option>
<option value="MY">Malaysia</option>
<option value="MV">Maldives</option>
<option value="ML">Mali</option>
<option value="MT">Malta</option>
<option value="MH">Marshall Islands</option>
<option value="MQ">Martinique</option>
<option value="MR">Mauritania</option>
<option value="MU">Mauritius</option>
<option value="YT">Mayotte</option>
<option value="MX">Mexico</option>
<option value="FM">Micronesia, Federated States of</option>
<option value="MD">Moldova</option>
<option value="MC">Monaco</option>
<option value="MN">Mongolia</option>
<option value="ME">Montenegro</option>
<option value="MS">Montserrat</option>
<option value="MA">Morocco</option>
<option value="MZ">Mozambique</option>
<option value="MM">Myanmar</option>
<option value="NA">Namibia</option>
<option value="NR">Nauru</option>
<option value="NP">Nepal</option>
<option value="NL">Netherlands</option>
<option value="NC">New Caledonia</option>
<option value="NZ">New Zealand</option>
<option value="NI">Nicaragua</option>
<option value="NE">Niger</option>
<option value="NG">Nigeria</option>
<option value="NU">Niue</option>
<option value="NF">Norfolk Island</option>
<option value="MP">Northern Mariana Islands</option>
<option value="NO">Norway</option>
<option value="OM">Oman</option>
<option value="PK">Pakistan</option>
<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
<option value="PA">Panama</option>
<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
<option value="PE">Peru</option>
<option value="PH">Philippines</option>
<option value="PN">Pitcairn</option>
<option value="PL">Poland</option>
<option value="PT">Portugal</option>
<option value="PR">Puerto Rico</option>
<option value="QA">Qatar</option>
<option value="RE">Réunion</option>
<option value="RO">Romania</option>
<option value="RU">Russian Federation</option>
<option value="RW">Rwanda</option>
<option value="BL">Saint Barthélemy</option>
<option value="SH">Saint Helena, Ascension and Tristan da Cunha</option>
<option value="KN">Saint Kitts and Nevis</option>
<option value="LC">Saint Lucia</option>
<option value="MF">Saint Martin (French part)</option>
<option value="PM">Saint Pierre and Miquelon</option>
<option value="VC">Saint Vincent and the Grenadines</option>
<option value="WS">Samoa</option>
<option value="SM">San Marino</option>
<option value="ST">Sao Tome and Principe</option>
<option value="SA">Saudi Arabia</option>
<option value="SN">Senegal</option>
<option value="RS">Serbia</option>
<option value="SC">Seychelles</option>
<option value="SL">Sierra Leone</option>
<option value="SG">Singapore</option>
<option value="SX">Sint Maarten (Dutch part)</option>
<option value="SK">Slovakia</option>
<option value="SI">Slovenia</option>
<option value="SB">Solomon Islands</option>
<option value="SO">Somalia</option>
<option value="ZA">South Africa</option>
<option value="GS">South Georgia and the South Sandwich Islands</option>
<option value="ES">Spain</option>
<option value="LK">Sri Lanka</option>
<option value="SD">Sudan</option>
<option value="SR">Suriname</option>
<option value="SS">South Sudan</option>
<option value="SJ">Svalbard and Jan Mayen</option>
<option value="SZ">Swaziland</option>
<option value="SE">Sweden</option>
<option value="CH">Switzerland</option>
<option value="SY">Syrian Arab Republic</option>
<option value="TW">Taiwan</option>
<option value="TJ">Tajikistan</option>
<option value="TZ">Tanzania</option>
<option value="TH">Thailand</option>
<option value="TL">Timor-Leste</option>
<option value="TG">Togo</option>
<option value="TK">Tokelau</option>
<option value="TO">Tonga</option>
<option value="TT">Trinidad and Tobago</option>
<option value="TN">Tunisia</option>
<option value="TR">Turkey</option>
<option value="TM">Turkmenistan</option>
<option value="TC">Turks and Caicos Islands</option>
<option value="TV">Tuvalu</option>
<option value="UG">Uganda</option>
<option value="UA">Ukraine</option>
<option value="AE">United Arab Emirates</option>
<option value="GB">United Kingdom</option>
<option value="US" selected="selected">United States</option>
<option value="UM">United States Minor Outlying Islands</option>
<option value="UY">Uruguay</option>
<option value="UZ">Uzbekistan</option>
<option value="VU">Vanuatu</option>
<option value="VE">Venezuela</option>
<option value="VN">Viet Nam</option>
<option value="VG">Virgin Islands, British</option>
<option value="VI">Virgin Islands, U.S.</option>
<option value="WF">Wallis and Futuna</option>
<option value="EH">Western Sahara</option>
<option value="YE">Yemen</option>
<option value="ZM">Zambia</option>
<option value="ZW">Zimbabwe</option>
</select><script>
$('#country_selector_quote-3009').selectize();
</script><br />
</div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">State</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][state]" id="state-3009" maxlength="3" type="text"/><br />
</div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/> </div>
</div>
<div class="row collapse" id="email_v">
<div class="small-3 large-2 columns">
<span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Comment</span>
</div>
<div class="small-9 large-10 columns">
<textarea name="data[Quote][comment]" placeholder="Additional comments" cols="30" rows="6" id="QuoteComment"></textarea> </div>
</div>
<!------------SERVICES PARTICULAR FORM START---------------->
<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->
<div class="row collapse">
<div class="small-3 large-2 columns">
</div>
<div class="small-9 large-10 columns">
<div class="recaptcha"><div id="recaptcha662b6dabac2b8"></div></div> </div>
</div>
<br />
<div class="row collapse">
<div class="small-3 large-2 columns">
</div>
<div class="small-9 large-10 columns">
<button id="submit_btn-3009" class="alert button expand" form="Quote-3009" type="submit">Contact me</button> </div>
</div>
</form><script>
var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c';
function postToPardot(formAction, id) {
$('#pardot-form-handler').load(function(){
$('#Quote-' + id).attr('action', formAction);
$('#Quote-' + id).submit();
});
$('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize());
}
$(document).ready(function() {
$('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>');
$('#Quote-3009').attr('action','javascript:postToPardot(\'' + $('#Quote-3009').attr('action') + '\', 3009)');
});
$("#Quote-3009 #submit_btn-3009").click(function (e) {
if($(this).closest('form')[0].checkValidity()){
e.preventDefault();
//disable the submit button
$("#Quote-3009 #submit_btn-3009").attr("disabled", true);
$("#Quote-3009 #submit_btn-3009").html("Processing...");
//submit the form
$("#Quote-3009").submit();
}
})
</script>
<script>
if ($("#Quote-3009 #country_selector_quote-3009.selectized").val() == 'US') {
var val = $("#state-3009").val();
$("#Quote-3009 #state-3009").replaceWith('<select name="data[Quote][state]" id="state-3009" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
if (val.length == 2) {
$("#state-3009").val(val);
}
$("#state-3009").parent().parent().show();
} else if ($("#Quote-3009 #country_selector_quote-3009.selectized").val() == 'CA') {
var val = $("#state-3009").val();
$("#Quote-3009 #state-3009").replaceWith('<select name="data[Quote][state]" id="state-3009" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
if (val.length == 2) {
$("#state-3009").val(val);
}
$("#state-3009").parent().parent().show();
} else if ($("#Quote-3009 #country_selector_quote-3009.selectized").val() == 'DE') {
var val = $("#state-3009").val();
$("#Quote-3009 #state-3009").replaceWith('<select name="data[Quote][state]" id="state-3009" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
if (val.length == 2) {
$("#state-3009").val(val);
}
$("#state-3009").parent().parent().show();
} else {
$("#Quote-3009 #state-3009").parent().parent().hide();
$("#Quote-3009 #state-3009").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3009" value="">');
}
$("#Quote-3009 #country_selector_quote-3009.selectized").change(function() {
if (this.value == 'US') {
$("#Quote-3009 #state-3009").replaceWith('<select name="data[Quote][state]" id="state-3009" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
$("#Quote-3009 #state-3009").parent().parent().show();
} else if (this.value == 'CA') {
$("#Quote-3009 #state-3009").replaceWith('<select name="data[Quote][state]" id="state-3009" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
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<div class="small-12 columns" >
<h6 style="height:60px">Megaruptor® 3</h6>
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<p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>
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<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
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<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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'description' => '<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center>
<h1 style="text-align: center;"><strong>PRE-RELEASE PROGRAM</strong></h1>
<h2 style="text-align: center;">Be involved in the optimization of the solution</h2>
<p style="text-align: center;">Handling <strong>highly viscous extracted DNA samples </strong>for any downstream steps and QC should not be a challenge anymore. The purpose is to combine the <strong>DNAFluid+ Kit </strong>with the <strong>Megaruptor 3</strong> in order to allow an effective and automatized homogenization of highly viscous DNA samples by keeping the integrity of the DNA and a full control of the DNA size prior any downstream applications.</p>
<p style="text-align: center;">You can be part of the improvement of the solution while getting an early access to the kit and giving us your comments, concerns, and suggestions.</p>
<h2 style="text-align: center;"><strong>How to participate ?</strong></h2>
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<p style="text-align: center;"><strong>Share your experience and protocols</strong></p>
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'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3>
<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p><strong>Storage</strong><br /> Store at room temperature</p>
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'description' => '<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center>
<h1 style="text-align: center;"><strong>PRE-RELEASE PROGRAM</strong></h1>
<h2 style="text-align: center;">Be involved in the optimization of the solution</h2>
<p style="text-align: center;">Handling <strong>highly viscous extracted DNA samples </strong>for any downstream steps and QC should not be a challenge anymore. The purpose is to combine the <strong>DNAFluid+ Kit </strong>with the <strong>Megaruptor 3</strong> in order to allow an effective and automatized homogenization of highly viscous DNA samples by keeping the integrity of the DNA and a full control of the DNA size prior any downstream applications.</p>
<p style="text-align: center;">You can be part of the improvement of the solution while getting an early access to the kit and giving us your comments, concerns, and suggestions.</p>
<h2 style="text-align: center;"><strong>How to participate ?</strong></h2>
<p style="text-align: center;">You can get an early access by ordering the DNAFluid+ kit as for other Diagenode products and feedback us on the product by clicking on the below button.</p>
<p style="text-align: center;"><strong>Share your experience and protocols</strong></p>
<p> </p>
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'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3>
<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>
<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
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<p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>',
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<th class="text-center" style="width: 296px; height: 55px;"></th>
<th class="text-center" style="width: 307px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" caption="false" width="120" height="83" />Megaruptor 3</th>
<th class="text-center" style="width: 329px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" alt="Megaruptor 2" caption="false" width="109" height="75" />Megaruptor 2</th>
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<td style="width: 296px; height: 38px;">Size range</td>
<td class="text-center" style="width: 307px; height: 38px;">5 - 100 kb</td>
<td class="text-center" style="width: 329px; height: 38px;">3 - 75 kb</td>
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<td class="text-center" style="width: 329px; height: 38px;">50 - 400 <span>µl</span></td>
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<td class="text-center" style="width: 307px; height: 38px;"><span><span>0 - 150 ng/</span></span><span><span>µl</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span><span>0 - 50 ng/</span></span><span><span>µl</span></span></td>
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<td style="width: 296px; height: 38px;">Throughput</td>
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<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
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<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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<div class="small-12 columns"><center><img src="../../img/partners/circulomics.png" /></center></div>
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'
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<div class="small-12 columns"><center><img src="../../img/partners/circulomics.png" /></center></div>
<p class="small" text-align="right"><a href="https://example.com">Circulomics</a></p>',
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'slug' => 'testimonial-dnafluidkit-circulomics',
'meta_keywords' => '',
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'modified' => '2020-12-10 16:40:33',
'created' => '2020-12-10 11:53:52',
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<h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Megaruptor<sup>®</sup> 3</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>
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<div class="small-12 columns" >
<h6 style="height:60px">Megaruptor® 3</h6>
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<p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>
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<p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>',
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<th class="text-center" style="width: 307px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" caption="false" width="120" height="83" />Megaruptor 3</th>
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<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
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<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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'description' => '<p>The Megaruptor 3 shearing kitには、すぐに使えるHydropore-SyringeとHydro Tubesの合計16サンプルが含まれています。 Shearing kitはMegaruptor®3（B06010003）と併用するよう特別設計になっており、<strong>5 kbから100 kb以上</strong>の範囲で再現性のあるDNA断片を生成が可能です。</p>
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'description' => '<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center>
<h1 style="text-align: center;"><strong>PRE-RELEASE PROGRAM</strong></h1>
<h2 style="text-align: center;">Be involved in the optimization of the solution</h2>
<p style="text-align: center;">Handling <strong>highly viscous extracted DNA samples </strong>for any downstream steps and QC should not be a challenge anymore. The purpose is to combine the <strong>DNAFluid+ Kit </strong>with the <strong>Megaruptor 3</strong> in order to allow an effective and automatized homogenization of highly viscous DNA samples by keeping the integrity of the DNA and a full control of the DNA size prior any downstream applications.</p>
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<h2 style="text-align: center;"><strong>How to participate ?</strong></h2>
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<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
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<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<h2 style="text-align: center;">Be involved in the optimization of the solution</h2>
<p style="text-align: center;">Handling <strong>highly viscous extracted DNA samples </strong>for any downstream steps and QC should not be a challenge anymore. The purpose is to combine the <strong>DNAFluid+ Kit </strong>with the <strong>Megaruptor 3</strong> in order to allow an effective and automatized homogenization of highly viscous DNA samples by keeping the integrity of the DNA and a full control of the DNA size prior any downstream applications.</p>
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<p style="text-align: center;"><strong>Share your experience and protocols</strong></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
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<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
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<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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'description' => '<p>The Megaruptor 3 shearing kitには、すぐに使えるHydropore-SyringeとHydro Tubesの合計16サンプルが含まれています。 Shearing kitはMegaruptor®3（B06010003）と併用するよう特別設計になっており、<strong>5 kbから100 kb以上</strong>の範囲で再現性のあるDNA断片を生成が可能です。</p>
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<p><strong>Storage</strong><br />Store at room temperature</p>
<p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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<h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Megaruptor<sup>®</sup> 3</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>
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<div class="small-9 large-10 columns">
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</div>
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<div class="small-12 columns" >
<h6 style="height:60px">Megaruptor® 3</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '3009',
'antibody_id' => null,
'name' => 'Megaruptor<sup>®</sup> 3',
'description' => '<p><a href="https://go.diagenode.com/l/928883/2023-04-06/3jhlc"><img src="https://www.diagenode.com/img/banners/pacbio-mega-promo-2023.png" /></a></p>
<p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>
<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p>',
'label1' => 'Demonstrated shearing of DNA from 5 -100 kb',
'info1' => '<h3>Excellent reproducibility and size distribution achieved with the Megaruptor 3</h3>
<center><img src="https://www.diagenode.com/img/product/shearing_technologies/mega3-results.png" /></center>
<p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>',
'label2' => 'Specifications for the Megaruptor',
'info2' => '<table style="width: 942px;">
<tbody>
<tr style="height: 55px;">
<th class="text-center" style="width: 296px; height: 55px;"></th>
<th class="text-center" style="width: 307px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" caption="false" width="120" height="83" />Megaruptor 3</th>
<th class="text-center" style="width: 329px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" alt="Megaruptor 2" caption="false" width="109" height="75" />Megaruptor 2</th>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Size range</td>
<td class="text-center" style="width: 307px; height: 38px;">5 - 100 kb</td>
<td class="text-center" style="width: 329px; height: 38px;">3 - 75 kb</td>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Volume range</td>
<td class="text-center" style="width: 307px; height: 38px;">65 - 500 <span>µl</span></td>
<td class="text-center" style="width: 329px; height: 38px;">50 - 400 <span>µl</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Concentration range</td>
<td class="text-center" style="width: 307px; height: 38px;"><span><span>0 - 150 ng/</span></span><span><span>µl</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span><span>0 - 50 ng/</span></span><span><span>µl</span></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Throughput</td>
<td class="text-center" style="width: 307px; height: 38px;"><span><span>1 to 8 samples in parallel</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span>1 to 2 samples in series</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Touchscreen</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
</tr>
<tr style="height: 56px;">
<td style="width: 296px; height: 56px;">Eliminate cross-contamination</td>
<td class="text-center" style="width: 307px; height: 56px;">with disposable elements in a completely closed-system</td>
<td class="text-center" style="width: 329px; height: 56px;">with disposable elements and with washing sequences</td>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Multiple-orifice disposables</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
</tr>
<tr style="height: 32px;">
<td style="width: 296px; height: 32px;">Consumables</td>
<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
<td class="text-center" style="width: 329px; height: 32px; text-align: center;">
<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'Video',
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'featured' => true,
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'slug' => 'megaruptor-3',
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'modified' => '2023-04-06 14:32:56',
'created' => '2018-12-20 16:38:16',
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'id' => '3018',
'antibody_id' => null,
'name' => 'Megaruptor 3 Shearing Kit',
'description' => '<p>The Megaruptor 3 shearing kitには、すぐに使えるHydropore-SyringeとHydro Tubesの合計16サンプルが含まれています。 Shearing kitはMegaruptor®3（B06010003）と併用するよう特別設計になっており、<strong>5 kbから100 kb以上</strong>の範囲で再現性のあるDNA断片を生成が可能です。</p>
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<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '',
'info1' => '<p>Sterile <span>Hydropores, Syringes, and Hydro Tubes</span> are intended for single use</p>
<div id="info1" class="content active">
<p><strong>Storage</strong><br />Store at room temperature</p>
<p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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'sf_code' => 'E07010003-',
'type' => 'ACC',
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'price_EUR' => '370',
'price_USD' => '390',
'price_GBP' => '300',
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'country' => 'ALL',
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'slug' => 'megaruptor-3-shearing-kit',
'meta_title' => 'Megaruptor Shearing kit',
'meta_keywords' => '',
'meta_description' => 'Megaruptor Shearing kit',
'modified' => '2023-03-31 13:53:54',
'created' => '2019-03-07 12:37:06',
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$document = array(
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'name' => 'Using the DNAFluid+ Kit for viscous DNA to effectively pre-condition high molecular weight DNA for consistent shearing',
'description' => '<p>This application note demonstrates that the PacBio Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition viscous DNA for Long Hydropore shearing without clogging.</p>',
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'type' => 'Application Note',
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'slug' => 'application-note-dnafluid.pdf',
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'modified' => '2022-03-24 09:57:10',
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)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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