Diagenode

Megaruptor® 3





PUB FLASH
Bcl11b, a novel GATA3-interacting protein, suppresses Th1...

CELL DIFFERENTIATION
Tight fragment size distribution
Hydropores eliminate clogging
High quality libraires
1 to 8 samples in parallel
Tuneable to between 5 - 100 kb
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カタログ番号
フォーマット
B06010003
1 unit

Designed to provide simple, automated, and highly reproducible fragmentation of DNA from 5kb to 100kb, the Megaruptor is essential for long-read de novo sequencing, detecting structural variants, and single molecule sequencing. The Megaruptor’s unique design provides state-of-the-art shearing performance which is is independent of the source, concentration, temperature, or salt content of a DNA sample, providing researcher with ultimate flexibility and ease of use. Safe from contamination and clogs, the Megaruptor seamlessly can process a flexible throughput of 1 to 8 samples simultaneously.

  • User-friendly touchscreen interface
  • High throughput: Process 1 to 8 samples simultaneously
  • Automated: Software allows for processing without user input; no washing sequence needed
  • Safe: Completely closed system with disposable non-clogging hydropores
  • Unmatched quality: No other system provides the tight fragment distribution you need
  • Ergonomic and space-efficient: Small footprint and low noise

TESTIMONIAL

The NGS Competence Center Tübingen (NCCT), together with four other national centers, has been established by the DFG (German Research Foundation) to support research projects with diverse needs of high-throughput sequencing technologies.

Long-read sequencing is very helpful to answer scientific questions in various topics such as microbiology or clinical research. We have noticed that the data yield of Nanopore sequencing can be notably increased by shearing the high molecular weight genomic DNA with an average size distribution of ~30kb and obtaining a read length N50 of 30kb. In this context, the Megaruptor 3 was critical to achieve long, homogenous and reproducible DNA preparation.

Megaruptor 3 is able to shear different molecular weight ranges up to 100kb; provided the input genomic DNA is of high-molecular weight. We have tested the Megaruptor 3 with genomic DNA from human blood, fibroblasts and difficult samples such as bacterial genomic DNA with high viscosity. With the Megaruptor 3 we have easily sheared up to 8 samples in parallel, saving preparation time. We have tested concentrations as low as 5 ng/µL and up to 70 ng/µL, saving sample material for optimization and meeting downstream requirements for library preparation.  

Finally, handling of the Megaruptor 3 is quick, with a simple interface. Diagenode is fast in delivering consumables and these are ready-to-go. Sample preparation requires one pipetting step. You need to enter 2 parameters of your sample: volume and concentration in addition to the speed related to your desired size. It is a safe process without sample cross-contamination. It is easy to control whether the sample is going through the hydropore. The system is fast; for the concentration and speed conditions we have tested, runs were completed between half an hour and 2 hours.

Elena Buena Atienza and Dr. Nicolas Casadei, Institute of Medical Genetics and Applied Genomics, University Clinics Tübingen
  • Demonstrated shearing of DNA from 5 -100 kb

    Excellent reproducibility and size distribution achieved with the Megaruptor 3

    A: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. B: DNA samples sheared at different speed settings of the Megaruptor were analyzed by pulse-field gel electrophoresis in 1% agarose gel. (NS: No Sheared)

  •  実験手法
    次世代シーケンシング
    DNA断片化、ライブラリー調製、自動化:NGSのワンストップショップ 1. 断片化装置を選択してください:150 bp〜75 kbの範囲でDNAを断片化します。 5μlまで断片化:150 bp〜2 kbNGS DNAライブラリー調製およびFFPE核酸抽出に最適で、 2 kb〜75 kbの範囲をできます。メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで ... Read more
    DNA/RNA断片
    近年の次世代シーケンシング(NGS)の進歩はゲノミクスや生物学に革命をもたらしました。 この成長により、最適なサンプル調製、ゲノムライブラリー構築には欠かせない高度技術の要求、必要性がさらに高まっています。 最適なライブラリー調製に最も重要な要因の一つは、シーケンシングされるDNAの品質です。 まずは、DNAを効果的かつ一貫してシーケンシングプラットフォームに応じた適切な断片サイズに切断し、具体的で信頼できるNGS結果を実現する必要があります。 Picoruptor and Megar... Read more
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    Megaruptor® 3 FLYER
    Your partner in long-read sequencing Designed to provide simple and automated DNA sample prepara...
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