Megaruptor® 3 旨在提供 5 kb - 100 kb DNA 片段化的最佳体验。剪切性能与 DNA 样品的来源、浓度、温度或盐含量无关。我们的用户友好系统可实现同时处理 8 份样品而无需用户额外进样。只需设置所需的参数，自动化系统即可完成其余工作。通过 Megaruptor 进行剪切可使用 PacBio® 和 Oxford Nanopore™ 技术系统实现最佳长读测序。
TESTIMONIALAs a PacBio Certified Service Provider it is critical that sample processing in my laboratory is precise and reproducible. For genome sequencing projects, the fragmentation of genomic DNA to precise and reproducible sizes is essential in order to optimize conditions for library preparation, sequencing, and downstream assembly. For this my laboratory relies on the Megaruptor system. The Megaruptor is the optimal system for long DNA fragment generation and tight fragment length distribution.
Brewster Kingham, Delaware Biotechnology Institute, University of Delaware
TESTIMONIALThe NGS Competence Center Tübingen (NCCT), together with four other national centers, has been established by the DFG (German Research Foundation) to support research projects with diverse needs of high-throughput sequencing technologies.
Long-read sequencing is very helpful to answer scientific questions in various topics such as microbiology or clinical research. We have noticed that the data yield of Nanopore sequencing can be notably increased by shearing the high molecular weight genomic DNA with an average size distribution of ~30kb and obtaining a read length N50 of 30kb. In this context, the Megaruptor 3 was critical to achieve long, homogenous and reproducible DNA preparation.
Megaruptor 3 is able to shear different molecular weight ranges up to 100kb; provided the input genomic DNA is of high-molecular weight. We have tested the Megaruptor 3 with genomic DNA from human blood, fibroblasts and difficult samples such as bacterial genomic DNA with high viscosity. With the Megaruptor 3 we have easily sheared up to 8 samples in parallel, saving preparation time. We have tested concentrations as low as 5 ng/µL and up to 70 ng/µL, saving sample material for optimization and meeting downstream requirements for library preparation.
Finally, handling of the Megaruptor 3 is quick, with a simple interface. Diagenode is fast in delivering consumables and these are ready-to-go. Sample preparation requires one pipetting step. You need to enter 2 parameters of your sample: volume and concentration in addition to the speed related to your desired size. It is a safe process without sample cross-contamination. It is easy to control whether the sample is going through the hydropore. The system is fast; for the concentration and speed conditions we have tested, runs were completed between half an hour and 2 hours.
Elena Buena Atienza and Dr. Nicolas Casadei, Institute of Medical Genetics and Applied Genomics, University Clinics Tübingen
A: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. B: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)
| Megaruptor 3 |
 Megaruptor 2 |
|
|---|---|---|
| Size range | 5 - 100 kb | 3 - 75 kb |
| Volume range | 65 - 500 µl | 50 - 400 µl |
| Concentration range | 0 - 150 ng/µl | 0 - 50 ng/µl |
| Throughput | 1 to 8 samples in parallel | 1 to 2 samples in series |
| Touchscreen | ||
| Eliminate cross-contamination | with disposable elements in a completely closed-system | with disposable elements and with washing sequences |
| Multiple-orifice disposables | ||
| Consumables | Megaruptor 3 Shearing Kit : Cat. No. E07010003 |
Hydropore - short : Cat. No. E07010001 |
 How to properly cite this product in your workDiagenode strongly recommends using this: Megaruptor® 3 (Diagenode Cat# B06010003). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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