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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>
<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
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<p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>',
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<tr style="height: 55px;">
<th class="text-center" style="width: 296px; height: 55px;"></th>
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<td style="width: 296px; height: 38px;">Volume range</td>
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<td style="width: 296px; height: 38px;">Concentration range</td>
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<td class="text-center" style="width: 329px; height: 38px;"><span><span>0 - 50 ng/</span></span><span><span>µl</span></span></td>
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<td class="text-center" style="width: 307px; height: 38px;"><span><span>1 to 8 samples in parallel</span></span></td>
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<td style="width: 296px; height: 38px;">Touchscreen</td>
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<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<td style="width: 296px; height: 56px;">Eliminate cross-contamination</td>
<td class="text-center" style="width: 307px; height: 56px;">with disposable elements in a completely closed-system</td>
<td class="text-center" style="width: 329px; height: 56px;">with disposable elements and with washing sequences</td>
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<td style="width: 296px; height: 38px;">Multiple-orifice disposables</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<td style="width: 296px; height: 32px;">Consumables</td>
<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
<td class="text-center" style="width: 329px; height: 32px; text-align: center;">
<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
<p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>',
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'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3>
<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p>
<p><strong>Storage</strong><br /> Store at room temperature</p>
<p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div>
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<div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div>
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<div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div>
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<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
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<p class="p1">Designed to provide simple and automated <span class="s1">DNA sample preparation</span>, the Megaruptor gives state-ofthe-art shearing performance which is independent of the source, concentration, temperature, or salt content of a DNA sample, providing researcher with ultimate flexibility and ease of use. The shearing with the Megaruptor leads to optimal long-read sequencing using <span class="s1">PacBio</span><span class="s2">®</span>, and <span class="s1">Oxford Nanopore™ </span>technologies.</p>',
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p>
<p><strong>Storage</strong><br /> Store at room temperature</p>
<p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p>
<p><strong>Storage</strong><br /> Store at room temperature</p>
<p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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<p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>
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<th class="text-center" style="width: 296px; height: 55px;"></th>
<th class="text-center" style="width: 307px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" caption="false" width="120" height="83" />Megaruptor 3</th>
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<td style="width: 296px; height: 56px;">Eliminate cross-contamination</td>
<td class="text-center" style="width: 307px; height: 56px;">with disposable elements in a completely closed-system</td>
<td class="text-center" style="width: 329px; height: 56px;">with disposable elements and with washing sequences</td>
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<td style="width: 296px; height: 32px;">Consumables</td>
<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
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<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p>
<p><strong>Storage</strong><br /> Store at room temperature</p>
<p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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<div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div>
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<div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div>
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<div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div>
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<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
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<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p><strong>Storage</strong><br /> Store at room temperature</p>
<p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<td style="width: 296px; height: 38px;">Volume range</td>
<td class="text-center" style="width: 307px; height: 38px;">65 - 500 <span>µl</span></td>
<td class="text-center" style="width: 329px; height: 38px;">50 - 400 <span>µl</span></td>
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<td style="width: 296px; height: 38px;">Concentration range</td>
<td class="text-center" style="width: 307px; height: 38px;"><span><span>0 - 150 ng/</span></span><span><span>µl</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span><span>0 - 50 ng/</span></span><span><span>µl</span></span></td>
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<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Throughput</td>
<td class="text-center" style="width: 307px; height: 38px;"><span><span>1 to 8 samples in parallel</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span>1 to 2 samples in series</span></td>
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<td style="width: 296px; height: 38px;">Touchscreen</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<td style="width: 296px; height: 56px;">Eliminate cross-contamination</td>
<td class="text-center" style="width: 307px; height: 56px;">with disposable elements in a completely closed-system</td>
<td class="text-center" style="width: 329px; height: 56px;">with disposable elements and with washing sequences</td>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Multiple-orifice disposables</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<td style="width: 296px; height: 32px;">Consumables</td>
<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
<td class="text-center" style="width: 329px; height: 32px; text-align: center;">
<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p>
<p><strong>Storage</strong><br /> Store at room temperature</p>
<p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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<div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div>
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<div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div>
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<div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div>
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<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3>
<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p>
<p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p>
<p><strong>Storage</strong><br /> Store at room temperature</p>
<p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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'description' => '<p>The Megaruptor 3 shearing kitには、すぐに使えるHydropore-SyringeとHydro Tubesの合計16サンプルが含まれています。 Shearing kitはMegaruptor®3（B06010003）と併用するよう特別設計になっており、<strong>5 kbから100 kb以上</strong>の範囲で再現性のあるDNA断片を生成が可能です。</p>
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<p><strong>Storage</strong><br />Store at room temperature</p>
<p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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<span class="success label" style="">B06010003</span>
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<h2>Contact Information</h2>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>
<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p>',
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<center><img src="https://www.diagenode.com/img/product/shearing_technologies/mega3-results.png" /></center>
<p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>',
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<th class="text-center" style="width: 296px; height: 55px;"></th>
<th class="text-center" style="width: 307px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" caption="false" width="120" height="83" />Megaruptor 3</th>
<th class="text-center" style="width: 329px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" alt="Megaruptor 2" caption="false" width="109" height="75" />Megaruptor 2</th>
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<td class="text-center" style="width: 329px; height: 38px;">3 - 75 kb</td>
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<td class="text-center" style="width: 329px; height: 38px;">50 - 400 <span>µl</span></td>
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<td style="width: 296px; height: 38px;">Concentration range</td>
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<td class="text-center" style="width: 329px; height: 38px;"><span><span>0 - 50 ng/</span></span><span><span>µl</span></span></td>
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<td class="text-center" style="width: 307px; height: 38px;"><span><span>1 to 8 samples in parallel</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span>1 to 2 samples in series</span></td>
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<td style="width: 296px; height: 38px;">Touchscreen</td>
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<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<td style="width: 296px; height: 56px;">Eliminate cross-contamination</td>
<td class="text-center" style="width: 307px; height: 56px;">with disposable elements in a completely closed-system</td>
<td class="text-center" style="width: 329px; height: 56px;">with disposable elements and with washing sequences</td>
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<td style="width: 296px; height: 38px;">Multiple-orifice disposables</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<td style="width: 296px; height: 32px;">Consumables</td>
<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
<td class="text-center" style="width: 329px; height: 32px; text-align: center;">
<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p>
<p><strong>Storage</strong><br /> Store at room temperature</p>
<p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div>
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<div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div>
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<div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div>
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<div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div>
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<p class="p1">Designed to provide simple and automated <span class="s1">DNA sample preparation</span>, the Megaruptor gives state-ofthe-art shearing performance which is independent of the source, concentration, temperature, or salt content of a DNA sample, providing researcher with ultimate flexibility and ease of use. The shearing with the Megaruptor leads to optimal long-read sequencing using <span class="s1">PacBio</span><span class="s2">®</span>, and <span class="s1">Oxford Nanopore™ </span>technologies.</p>',
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3>
<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p>
<p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p>
<p><strong>Storage</strong><br /> Store at room temperature</p>
<p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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'name' => 'Megaruptor 3 Shearing Kit',
'description' => '<p>The Megaruptor 3 shearing kitには、すぐに使えるHydropore-SyringeとHydro Tubesの合計16サンプルが含まれています。 Shearing kitはMegaruptor®3（B06010003）と併用するよう特別設計になっており、<strong>5 kbから100 kb以上</strong>の範囲で再現性のあるDNA断片を生成が可能です。</p>
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<p><strong>Storage</strong><br />Store at room temperature</p>
<p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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<span class="success label" style="">B06010003</span>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
<p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>',
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'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3>
<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p>
<p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p>
<p><strong>Storage</strong><br /> Store at room temperature</p>
<p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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