Optimal long DNA fragment generation prior to SMRTbell® library preparation
Several methods exist today for shearing genomic DNA, but few are reliable for generating DNA fragments >50 kb necessary for assembly of many microbial, human, plant and animal genomes de novo. Pacific Biosciences highly recommends the MegaruptorTM System for shearing genomic DNA to large fragments for long-read sequencing in the Sequel® System.
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