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<p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>
<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
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<p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>',
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<th class="text-center" style="width: 296px; height: 55px;"></th>
<th class="text-center" style="width: 307px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" caption="false" width="120" height="83" />Megaruptor 3</th>
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<td class="text-center" style="width: 307px; height: 38px;">5 - 100 kb</td>
<td class="text-center" style="width: 329px; height: 38px;">3 - 75 kb</td>
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<td style="width: 296px; height: 38px;">Volume range</td>
<td class="text-center" style="width: 307px; height: 38px;">65 - 500 <span>µl</span></td>
<td class="text-center" style="width: 329px; height: 38px;">50 - 400 <span>µl</span></td>
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<td style="width: 296px; height: 38px;">Concentration range</td>
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<td class="text-center" style="width: 329px; height: 38px;"><span><span>0 - 50 ng/</span></span><span><span>µl</span></span></td>
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<td class="text-center" style="width: 307px; height: 38px;"><span><span>1 to 8 samples in parallel</span></span></td>
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<td style="width: 296px; height: 38px;">Touchscreen</td>
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<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<td style="width: 296px; height: 56px;">Eliminate cross-contamination</td>
<td class="text-center" style="width: 307px; height: 56px;">with disposable elements in a completely closed-system</td>
<td class="text-center" style="width: 329px; height: 56px;">with disposable elements and with washing sequences</td>
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<td style="width: 296px; height: 38px;">Multiple-orifice disposables</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<td style="width: 296px; height: 32px;">Consumables</td>
<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
<td class="text-center" style="width: 329px; height: 32px; text-align: center;">
<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
<p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>',
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<p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p>
<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<div class="small-12 columns"><center><img src="../../img/partners/circulomics.png" /></center></div>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<tbody>
<tr style="height: 55px;">
<th class="text-center" style="width: 296px; height: 55px;"></th>
<th class="text-center" style="width: 307px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" caption="false" width="120" height="83" />Megaruptor 3</th>
<th class="text-center" style="width: 329px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" alt="Megaruptor 2" caption="false" width="109" height="75" />Megaruptor 2</th>
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<td style="width: 296px; height: 38px;">Size range</td>
<td class="text-center" style="width: 307px; height: 38px;">5 - 100 kb</td>
<td class="text-center" style="width: 329px; height: 38px;">3 - 75 kb</td>
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<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Volume range</td>
<td class="text-center" style="width: 307px; height: 38px;">65 - 500 <span>µl</span></td>
<td class="text-center" style="width: 329px; height: 38px;">50 - 400 <span>µl</span></td>
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<td style="width: 296px; height: 38px;">Concentration range</td>
<td class="text-center" style="width: 307px; height: 38px;"><span><span>0 - 150 ng/</span></span><span><span>µl</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span><span>0 - 50 ng/</span></span><span><span>µl</span></span></td>
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<td class="text-center" style="width: 307px; height: 38px;"><span><span>1 to 8 samples in parallel</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span>1 to 2 samples in series</span></td>
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<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Touchscreen</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<tr style="height: 56px;">
<td style="width: 296px; height: 56px;">Eliminate cross-contamination</td>
<td class="text-center" style="width: 307px; height: 56px;">with disposable elements in a completely closed-system</td>
<td class="text-center" style="width: 329px; height: 56px;">with disposable elements and with washing sequences</td>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Multiple-orifice disposables</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<tr style="height: 32px;">
<td style="width: 296px; height: 32px;">Consumables</td>
<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
<td class="text-center" style="width: 329px; height: 32px; text-align: center;">
<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
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<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
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<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
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<td style="width: 296px; height: 38px;">Size range</td>
<td class="text-center" style="width: 307px; height: 38px;">5 - 100 kb</td>
<td class="text-center" style="width: 329px; height: 38px;">3 - 75 kb</td>
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<td style="width: 296px; height: 38px;">Volume range</td>
<td class="text-center" style="width: 307px; height: 38px;">65 - 500 <span>µl</span></td>
<td class="text-center" style="width: 329px; height: 38px;">50 - 400 <span>µl</span></td>
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<td style="width: 296px; height: 38px;">Concentration range</td>
<td class="text-center" style="width: 307px; height: 38px;"><span><span>0 - 150 ng/</span></span><span><span>µl</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span><span>0 - 50 ng/</span></span><span><span>µl</span></span></td>
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<td style="width: 296px; height: 38px;">Throughput</td>
<td class="text-center" style="width: 307px; height: 38px;"><span><span>1 to 8 samples in parallel</span></span></td>
<td class="text-center" style="width: 329px; height: 38px;"><span>1 to 2 samples in series</span></td>
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<td style="width: 296px; height: 38px;">Touchscreen</td>
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<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<td style="width: 296px; height: 56px;">Eliminate cross-contamination</td>
<td class="text-center" style="width: 307px; height: 56px;">with disposable elements in a completely closed-system</td>
<td class="text-center" style="width: 329px; height: 56px;">with disposable elements and with washing sequences</td>
</tr>
<tr style="height: 38px;">
<td style="width: 296px; height: 38px;">Multiple-orifice disposables</td>
<td class="text-center" style="width: 307px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
<td class="text-center" style="width: 329px; height: 38px;"><i class="fa fa-check green" aria-hidden="true"></i></td>
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<td style="width: 296px; height: 32px;">Consumables</td>
<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
<td class="text-center" style="width: 329px; height: 32px; text-align: center;">
<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
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<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
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<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<a href="/en/p/megaruptor-3"><img src="/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" class="th"/></a> </div>
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<span class="success label" style="">B06010003</span>
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<p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p>
<center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
<p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p>',
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<p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>',
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<th class="text-center" style="width: 307px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" caption="false" width="120" height="83" />Megaruptor 3</th>
<th class="text-center" style="width: 329px; height: 55px;"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" alt="Megaruptor 2" caption="false" width="109" height="75" />Megaruptor 2</th>
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<td class="text-center" style="width: 307px; height: 32px;"><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Megaruptor 3 Shearing Kit : </a><br /><a href="https://www.diagenode.com/en/p/megaruptor-3-shearing-kit">Cat. No. E07010003</a></td>
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<p style="text-align: center;"><a href="https://www.diagenode.com/p/hydropore-short-10-pc"> Hydropore - short : Cat. No. E07010001 </a> <br /> <a href="https://www.diagenode.com/en/p/hydropore-long-10-pc"> Hydropore - long : Cat. No. E07010002 </a><br /><a href="https://www.diagenode.com/en/p/hydro-tubes-50-pc">Hydro Tubes : Cat. No. C30010018</a></p>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2>
<p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p>
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<h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p>
<p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p>
<h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2>
<p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p>
<p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p>
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<h6 style="height:60px">Megaruptor® 3</h6>
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$related = array(
'id' => '3018',
'antibody_id' => null,
'name' => 'Megaruptor 3 Shearing Kit',
'description' => '<p>The Megaruptor 3 shearing kitには、すぐに使えるHydropore-SyringeとHydro Tubesの合計16サンプルが含まれています。 Shearing kitはMegaruptor®3（B06010003）と併用するよう特別設計になっており、<strong>5 kbから100 kb以上</strong>の範囲で再現性のあるDNA断片を生成が可能です。</p>
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<p><strong>Storage</strong><br />Store at room temperature</p>
<p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
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'info2' => '<center><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
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'label3' => '',
'info3' => '<p></p>
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'format' => '16 samples',
'catalog_number' => 'E07010003',
'old_catalog_number' => '',
'sf_code' => 'E07010003-',
'type' => 'ACC',
'search_order' => '',
'price_EUR' => '370',
'price_USD' => '390',
'price_GBP' => '300',
'price_JPY' => '57960',
'price_CNY' => '',
'price_AUD' => '975',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'megaruptor-3-shearing-kit',
'meta_title' => 'Megaruptor Shearing kit',
'meta_keywords' => '',
'meta_description' => 'Megaruptor Shearing kit',
'modified' => '2023-03-31 13:53:54',
'created' => '2019-03-07 12:37:06',
'ProductsRelated' => array(
'id' => '4829',
'product_id' => '3219',
'related_id' => '3018'
),
'Image' => array(
(int) 0 => array(
'id' => '1794',
'name' => 'product/shearing_technologies/E0701003-mega3-shearing-kit.jpg',
'alt' => 'megaruptor 3 shearing kit',
'modified' => '2019-03-07 13:38:16',
'created' => '2019-03-07 13:38:16',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3018',
'antibody_id' => null,
'name' => 'Megaruptor 3 Shearing Kit',
'description' => '<p>The Megaruptor 3 shearing kitには、すぐに使えるHydropore-SyringeとHydro Tubesの合計16サンプルが含まれています。 Shearing kitはMegaruptor®3（B06010003）と併用するよう特別設計になっており、<strong>5 kbから100 kb以上</strong>の範囲で再現性のあるDNA断片を生成が可能です。</p>
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<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '',
'info1' => '<p>Sterile <span>Hydropores, Syringes, and Hydro Tubes</span> are intended for single use</p>
<div id="info1" class="content active">
<p><strong>Storage</strong><br />Store at room temperature</p>
<p><strong>Precautions</strong><br />This product is for research use only. Not for use in diagnostic or therapeutic procedures</p>
</div>
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'label2' => '',
'info2' => '<center><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>
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'label3' => '',
'info3' => '<p></p>
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'format' => '16 samples',
'catalog_number' => 'E07010003',
'old_catalog_number' => '',
'sf_code' => 'E07010003-',
'type' => 'ACC',
'search_order' => '',
'price_EUR' => '370',
'price_USD' => '390',
'price_GBP' => '300',
'price_JPY' => '57960',
'price_CNY' => '',
'price_AUD' => '975',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'megaruptor-3-shearing-kit',
'meta_title' => 'Megaruptor Shearing kit',
'meta_keywords' => '',
'meta_description' => 'Megaruptor Shearing kit',
'modified' => '2023-03-31 13:53:54',
'created' => '2019-03-07 12:37:06',
'ProductsGroup' => array(
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'product_id' => '3018',
'group_id' => '355'
)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1042',
'name' => 'Megaruptor® 3 ',
'description' => '<p class="p1"><strong>Your partner in long-read sequencing</strong></p>
<p class="p1">Designed to provide simple and automated <span class="s1">DNA sample preparation</span>, the Megaruptor gives state-ofthe-art shearing performance which is independent of the source, concentration, temperature, or salt content of a DNA sample, providing researcher with ultimate flexibility and ease of use. The shearing with the Megaruptor leads to optimal long-read sequencing using <span class="s1">PacBio</span><span class="s2">®</span>, and <span class="s1">Oxford Nanopore™ </span>technologies.</p>',
'image_id' => '186',
'type' => 'flyer',
'url' => 'files/flyers/megaruptor3_flyer.pdf',
'slug' => 'megaruptor3-flyer',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2019-03-05 14:23:36',
'created' => '2019-02-19 15:45:41',
'ProductsDocument' => array(
'id' => '3218',
'product_id' => '3219',
'document_id' => '1042'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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