MeCP2 Antibody

Catalog Number
100 µl
  Bulk order

Alternative names: AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT

Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.

Concentrationnot determined
Species reactivityHuman
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 - 1:500 Fig 1
Western Blotting 1:2,000 Fig 2
  • Validation Data


    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2
    Figure 2A: Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder.
    Figure 2B: Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.

  •  Applications
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
    Datasheet MeCP2 CS-088-100 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
    MeCP2 Antibody SDS GB en Download
    MeCP2 Antibody SDS US en Download
    MeCP2 Antibody SDS BE nl Download
    MeCP2 Antibody SDS BE fr Download
    MeCP2 Antibody SDS FR fr Download
    MeCP2 Antibody SDS ES es Download
    MeCP2 Antibody SDS DE de Download
    MeCP2 Antibody SDS JP ja Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MeCP2 Antibody (Diagenode Cat# C15310088 Lot# A131-004 ). Click here to copy to clipboard.

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