Request a quote for a bulk order for MeCP2 polyclonal antibody - Pioneer. Please fill out the form here below. Your local sales account manager will get in touch with you
shortly and send you a quotation based on your requirements.
Alternative names: AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT
Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2 Figure 2A: Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. Figure 2B: Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
Learn more about: Load... Read more