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Figure 1. Western blot analysis using the Diagenode antibody directed against LSD1 Figure 1A: Western blot was performed with the Diagenode antibody against LSD1 (cat# CS-028-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk on purified recombinant LSD1 protein (50 and 25 ng, lane 1 and 2) and on nuclear extracts (50 μg) from HeLa and HEK293 cells (lane 3 and 4, respectively). Figure 1B: Western blot was performed as described above using nuclear extracts from HeLa cells (HeLa NE, 20 μg). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Figure 2. Western blot analysis using the Diagenode antibody directed against LSD1 Whole cell extracts (40 μg) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (cat. No. C15310028) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
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