Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against Inosine
RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 1 µg of the Diagenode monoclonal antibody against Inosine (cat. No. C15200251) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the Inosine antibody (right) The left panel shows the input. Figure 1B shows the gel image for the Inosine antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.