Diagenode

Inosine Antibody (sample size)

Catalog Number
Format
Price
C15200251-10
10 µg
$97.00
  Bulk order
Other format



Monoclonal antibody raised in mouse using Inosine (I) conjugated to BSA as immunogen.

Lot001
Concentration1.75 µg/µl
Species reactivityHuman, other (wide range): positive.
TypeMonoclonal
PurityProtein A purified monoclonal antibody.
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% sodium azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
RIP * 1 µg per IP Fig 1, 2 ,3

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 10 µg per IP.

  • Validation Data
    A.
    B.

    Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against Inosine
    RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 1 µg of the Diagenode monoclonal antibody against Inosine (cat. No. C15200251) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the Inosine antibody (right) The left panel shows the input. Figure 1B shows the gel image for the Inosine antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

    Figure 2. RIP using the Diagenode monoclonal antibody directed against Inosine
    RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against Inosine (cat. No. C15200251). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Figure 3. RIP using the Diagenode monoclonal antibody directed against Inosine
    RIP was performed with 1 µg of the Diagenode antibody against Inosine (cat. No. C15200251) on 40 µg total RNA from human HeLa cells was spiked with an in vitro produced RNA molecule containing Inosine nucleotides as well as an unmodified control RNA (100 ng each). IgG (1 µg/IP) as well as an m6A antibody (C15410208) were used as negative control. QRT-PCR was performed with primers specific for the Inosine and unmodified RNA molecules. Figure 3 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  •  Applications
  •  Documents
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Datasheet Inosine Antibody C15200251 DATASHEET
    Download
  •  Safety sheets
    Inosine Antibody SDS GB en Download
    Inosine Antibody SDS US en Download
    Inosine Antibody SDS DE de Download
    Inosine Antibody SDS JP ja Download
    Inosine Antibody SDS BE nl Download
    Inosine Antibody SDS BE fr Download
    Inosine Antibody SDS FR fr Download
    Inosine Antibody SDS ES es Download
  •  Publications

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