Inosine Antibody

Catalog Number
50 µg/29 µl
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Monoclonal antibody raised in mouse using Inosine (I) conjugated to BSA as immunogen.

Concentration2 µg/µl
Species reactivityHuman, other (wide range): positive.
PurityProtein A purified monoclonal antibody.
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% sodium azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
RIP * 1 µg per IP Fig 1

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 10 µg per IP.

  • Validation Data

    Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against Inosine
    IP was performed with the Diagenode antibody against Inosine (cat. No. C15200251) on 40 µg total RNA from human HeLa cells, spiked with an in vitro produced RNA molecule containing Inosine nucleotides as well as an unmodified control RNA (40 ng each). A titration of the antibody of 1, 2 and 5 µg was analyzed. IgG (2 µg/IP) was used as negative control.
    Figure 1A The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. The peak at ~500 bp corresponds to the Inosine spike in, whereas the negative control spike in (expected size ~300 bp) is not captured by the antibody.
    Figure 1B shows the gel image for the Inosine antibody (lane 1, 2 and 3), the IgG negative control (lane 4) and the input (lane 5). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA and of both spike in controls is indicated on the right.

  •  実験手法
  •  資料
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Datasheet Inosine Antibody C15200251 DATASHEET
  •  Safety sheets
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  •  出版物

    How to properly cite this product in your work

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    Jul 10-Jul 12, 2024


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