6X His epitope tag Antibody

Catalog Number
100 µg
  Bulk order

Monoclonal antibody raised in mouse against 6X His epitope tag, using a KLH-conjugated synthetic peptide.

Concentration1.0 μg/μl
Species reactivity
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:75,000
Western Blotting 1:1,000
Immunoprecipitation 1:200 Fig 1, 2
  • Validation Data


    Figure 1. 6X His Tag antibody ELISA results
    ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000.

    Western Blot

    Figure 2. 6X His Tag antibody western blot results
    Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT.

    Western Blot

    Figure 3. 6X His Tag antibody western blot results
    Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1.

  •  Applications
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunoprecipitation Read more
  •  Documents
  •  Publications

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