Diagenode

6X His epitope tag monoclonal antibody

Catalog Number
Format
Price
C15200208
100 µg
$440.00
  Bulk order



Monoclonal antibody raised in mouse against 6X His epitope tag, using a KLH-conjugated synthetic peptide.

Lot001
Concentration1.0 μg/μl
Species reactivity
TypeMonoclonal
PurityAffinity purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:75,000
Western Blotting 1:1,000
Immunoprecipitation 1:200 Fig 1, 2
  • Validation Data

    ELISA

    Figure 1. 6X His Tag antibody ELISA results
    ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000.

    Western Blot

    Figure 2. 6X His Tag antibody western blot results
    Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT.

    Western Blot

    Figure 3. 6X His Tag antibody western blot results
    Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1.

  •  実験手法
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
  •  資料
  •  Safety sheets
    SDS C15200208 6X His epitope tag Antibody US en Download
    SDS C15200208 6X His epitope tag Antibody GB en Download
    SDS C15200208 6X His epitope tag Antibody ES es Download
    SDS C15200208 6X His epitope tag Antibody DE de Download
    SDS C15200208 6X His epitope tag Antibody JP ja Download
    SDS C15200208 6X His epitope tag Antibody BE nl Download
    SDS C15200208 6X His epitope tag Antibody BE fr Download
    SDS C15200208 6X His epitope tag Antibody FR fr Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: 6X His epitope tag monoclonal antibody (Diagenode Cat# C15200208 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy