Applications

Protein-coding RNA with specific poly A selectionCancer-related mRNA signaturesAberrant mRNA translation in cancer pathogenesisGene expression profiling and identification of differentially expressed genes (DEG)Epigenetic mechanism of gene expression (transcriptome plus H3K36me3 and H3K4me3 marks)Functional annotati... Learn more

Transcriptome sequencing: Analysis of whole transcriptome and RNAs larger than 200nt with optional rRNA depletionNon-protein coding RNAs involved in chromatin remodeling & transcriptional/post-transcriptional regulation Learn more

Post-transcriptional regulation of gene expressionNovel transcripts and differentially-expressed small RNAsmiRNAs involved in regulation of onco- and tumor-suppressor gene expressionDifferential miRNA expression Learn more

Low input libraries from picograms  Specific analyses  Complete customized projects  Learn more

Use our services to explore ChIP-seq histone modifications down to 10,000 cellsPost-translational modification of histones regulates gene expression. With our Epigenomic Profiling Services, study regulatory elements and their interacting proteins with active promoter and enhancer analysis. Profile genome-wide histon... Learn more

Explore the effects of transcription factor binding through ChIP-seq analysis of a multitude of TFs including:CTCF: transcriptional repressor and insulator activityp300/CBP: histone acetyltransferasePol II, p53, and moreEpigenetic writers, readers, erasersNuclear receptorsTumor supressor genes Learn more

Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine D... Learn more

The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is ... Learn more

The MBD approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. The H6-GST-MBD fusion protein can be used to... Learn more

Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purificati... Learn more

We have what you need for a successful Western blot! Western blots require multiple steps - sample preparation, sample loading, electrophoresis, protein transfer, antibody incubation, and signal detection - so it is important to be sure that each step is done correctly. For a successful Western blot we offer: Loadin... Learn more

Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus o... Learn more

Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life s... Learn more

In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to... Learn more

Diagenode's high yields FFPE DNA extraction using Bioruptor® is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no toxic reagents, digest tissues, and purify DNA with high y... Learn more

Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to fragmented DNA or RNA prior to sequencing After input DNA has been fragmented, it is end-repaired and blunt-ended. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphoryl... Learn more

Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. Diageno... Learn more

Protein array Learn more

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Dot blotting Learn more

DIP Learn more

Enzyme-linked immunosorbent assay. Learn more

FISH Learn more

Flow cyt Learn more

Gel Supershift Learn more

Immunochemistry Learn more

Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°... Learn more

Immunohistochemistry Learn more

Immunoprecipitation Learn more

Peptide array Learn more

RIP Learn more

Epigenetic antibodies you can trust! Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-hist... Learn more

Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Loading control, MW marker visualization. Check our selection of antibod... Learn more