Applications

RNA-seq

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Messenger RNA (mRNA)
Protein-coding RNA with specific poly A selectionCancer-related mRNA signaturesAberrant mRNA translation in cancer pathogenesisGene expression profiling and identification of differentially expressed genes (DEG)Epigenetic mechanism of gene expression (transcriptome plus H3K36me3 and H3K4me3 marks)Functional annotati... Learn more

Whole transcriptome analysis and long non-coding RNA (lncRNA) analysis
Transcriptome sequencing: Analysis of whole transcriptome and RNAs larger than 200nt with optional rRNA depletionNon-protein coding RNAs involved in chromatin remodeling & transcriptional/post-transcriptional regulation Learn more

Small RNA and miRNA
Post-transcriptional regulation of gene expressionNovel transcripts and differentially-expressed small RNAsmiRNAs involved in regulation of onco- and tumor-suppressor gene expressionDifferential miRNA expression Learn more
Services

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Custom NGS services
Low input libraries from picograms Specific analyses Complete customized projects Learn more

Histone ChIP-seq services
Use our services to explore ChIP-seq histone modifications down to 10,000 cellsPost-translational modification of histones regulates gene expression. With our Epigenomic Profiling Services, study regulatory elements and their interacting proteins with active promoter and enhancer analysis. Profile genome-wide histon... Learn more

Transcription factor ChIP-seq services
Explore the effects of transcription factor binding through ChIP-seq analysis of a multitude of TFs including:CTCF: transcriptional repressor and insulator activityp300/CBP: histone acetyltransferasePol II, p53, and moreEpigenetic writers, readers, erasersNuclear receptorsTumor supressor genes Learn more
DNA Methylation

Complete solutions for DNA methylation studies Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements. DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. In vivo, DNA is methylated following DNA replication ... Learn more

Bisulfite conversion
Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine D... Learn more

Methylated DNA immunoprecipitation
The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is ... Learn more

Methylbinding domain protein
The MBD approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. The H6-GST-MBD fusion protein can be used to... Learn more
Chromatin shearing

The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, Diagenode's Bioruptor® uses state-of-the-art ultrasound ACT (Adaptive Cavitation Technology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor® provides a precisely controlled temperature environment that preserves chromatin from heat ... Learn more
Protein Analysis

Proteins play a critical role in virtually all cell processes including metabolism, structure, growth, and repair. Understanding protein function, behavior, structure, and their importance are key aspects of understanding disease and therapeutics. Experimental analysis of proteins typically involves expression and purification or the direct extraction of proteins from cells or tissues for further downstream analysis such as ligand binding assays, mass spectrometry, or protein sequencing. Other protein analysis methods include detection methods such as BCA assays and Western blots or methods to understand protein-to-protein in... Learn more

Protein extraction
Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purificati... Learn more

Western blot analysis
We have what you need for a successful Western blot! Western blots require multiple steps - sample preparation, sample loading, electrophoresis, protein transfer, antibody incubation, and signal detection - so it is important to be sure that each step is done correctly. For a successful Western blot we offer: Loadin... Learn more
Chromatin Analysis

Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression. ChIP involved the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a specific protein or protein modification are used to determine the relative abundance of the antigen at specific loci. ChIP-seq and ChIP-qPCR are techniques that allow the identification of protein-DNA binding sites. Check out how to do your ChIP yourself and what you will need Learn about why ChIP is... Learn more

ChIP-qPCR
Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus o... Learn more

ChIP-seq
Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life s... Learn more
Next Generation Sequencing

DNA Shearing, library preparation, and automation: your one-stop shop for NGS 1. Choose your shearing device: Shear DNA anywhere from 150 bp to 75 kb Shear down to 5 μl: 150 bp - 2 kb Perfect for NGS DNA library prep and FFPE nucleic acid extraction Shear anywhere from 2 kb – 75 kb Perfect for mate pair library prep and long fragment DNA sequencing Shear 20 or 50 µl with this light desktop device 2. Choose your optimized library preparation kit 3. Choose library prep automation for unmatched data reproducibility Low input of 50 pg: MicroPlex Library Preparation Input above ... Learn more

DNA/RNA shearing
In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to... Learn more

FFPE DNA extraction
Diagenode's high yields FFPE DNA extraction using Bioruptor® is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no toxic reagents, digest tissues, and purify DNA with high y... Learn more

DNA/RNA library preparation
Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to fragmented DNA or RNA prior to sequencing After input DNA has been fragmented, it is end-repaired and blunt-ended. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphoryl... Learn more

RNA extraction
Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. Diageno... Learn more
Antibody Applications

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Protein array
Protein array Learn more

ChIP-qPCR (ab)
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ChIP-seq (ab)
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DB
Dot blotting Learn more

DIP
DIP Learn more

ELISA
Enzyme-linked immunosorbent assay. Learn more

FISH
FISH Learn more

Flow Cyt
Flow cyt Learn more

GSA
Gel Supershift Learn more

IC
Immunochemistry Learn more

IF
Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°... Learn more

IHC
Immunohistochemistry Learn more

IP
Immunoprecipitation Learn more

Peptide array
Peptide array Learn more

RIP
RIP Learn more

siRNA Knockdown
Epigenetic antibodies you can trust! Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-hist... Learn more

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Loading control, MW marker visualization. Check our selection of antibod... Learn more

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