Complete solutions for DNA methylation studies Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements. DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. In vivo, DNA is methylated following DNA replication ... Learn more
The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, Diagenode's Bioruptor® uses state-of-the-art ultrasound ACT (Adaptive Cavitation Technology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor® provides a precisely controlled temperature environment that preserves chromatin from heat ... Learn more
Proteins play a critical role in virtually all cell processes including metabolism, structure, growth, and repair. Understanding protein function, behavior, structure, and their importance are key aspects of understanding disease and therapeutics. Experimental analysis of proteins typically involves expression and purification or the direct extraction of proteins from cells or tissues for further downstream analysis such as ligand binding assays, mass spectrometry, or protein sequencing. Other protein analysis methods include detection methods such as BCA assays and Western blots or methods to understand protein-to-protein in... Learn more
Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression. ChIP involved the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a specific protein or protein modification are used to determine the relative abundance of the antigen at specific loci. ChIP-seq and ChIP-qPCR are techniques that allow the identification of protein-DNA binding sites. Check out how to do your ChIP yourself and what you will need Learn about why ChIP is... Learn more
DNA Shearing, library preparation, and automation: your one-stop shop for NGS 1. Choose your shearing device: Shear DNA anywhere from 150 bp to 75 kb Shear down to 5 μl: 150 bp - 2 kb Perfect for NGS DNA library prep and FFPE nucleic acid extraction Shear anywhere from 2 kb – 75 kb Perfect for mate pair library prep and long fragment DNA sequencing Shear 20 or 50 µl with this light desktop device 2. Choose your optimized library preparation kit 3. Choose library prep automation for unmatched data reproducibility Low input of 50 pg: MicroPlex Library Preparation Input above ... Learn more
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