Diagenode provides kits with optimized reagents and simplified protocols for ChIP including the LowCell# ChIP Kit, HighCell# ChIP Kit, iDeal ChIP-seq kit and the True MicroChIP kit. This protocol describes the use of the LowCell# ChIP Kit which features:
One day protocol
Rapid DNA purification with a simplified DIB (DNA isolation buffer)
Ability to work with as little as 200 cells for histone ChIPs.
Reduction of reagent usage with fewer buffers
Fewer steps and reduced handling with magnetic procedure using our optimized DiaMag Magnetic Rack
Validation with our shearing kits and the Bioruptor® sonicator
A variety of control options with our ChIP-Seq and ChIP grade antibodies and negative IgG from rabbit or mouse.