Diagenode

MagMeDIP qPCR Kit

目录号
格式
价格
C02010020
(mc-magme-A10)
10 rxns (IP)
$380.00
其他格式

Sensitive tumour detection and classification using plasma cell-free DNA methylomes
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Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA
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Perform MeDIP (Methylated DNA Immunoprecipitation) followed by qPCR to estimate DNA methylation status of your sample using 5-methylcytosine antibody. Our kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.

To perform an NGS compatible MeDIP-seq assay, you will need to use the MagMeDIP-seq Package.

Additionally, the development of Diagenode’s MagMeDIP qPCR Kit has been largely inspired by the reproducibility standards reached using the SX-8G IP-Star® Automated System. If you are interested in automating this protocol, please check our Auto MagMeDIP qPCR Kit.

Characteristics

  • Generate highly consistent results with internal controls in 24h
  • Minimize error with many reagents in 1 tube
  • Optimized purification (DIB - DNA isolation buffer)
  • Allows direct correlation between IP’d material & methylation status

MagMeDIP kit validated using Bioruptor

Figure 1. IP results obtained with Diagenode MagMeDIP qPCR Kit. MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.