V5 Epitope tag polyclonal antibody - Classic

Catalog Number
100 μg
  Bulk order

Polyclonal antibody raised in rabbit against V5 Epitope tag, using a KLH-conjugated synthetic peptide.

Concentration1.0 μg/μl
Species reactivity
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:10,000 - 1:60,000
Immunohistochemistry/td> 1:500 - 1:3,000
Western Blotting 1:5,000 - 1:10,000 Fig 3
  • Validation Data

    Western blot results

    Figure 1. V5 Epitope tag antibody Western blot results
    The V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. The antibody was used at 1.0 μg/ml to detect 0.05 μg (lane 2) of full- length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS- PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4° C.

  • Applications
    Enzyme-linked immunosorbent assay. Read more
    Immunohistochemistry Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet V5 Epitope tag C15410270 DATASHEET
    Datasheet description
  • Publications

    How to properly cite this product in your work

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