Diagenode

TDG monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200227
50 μg
$295.00
  Bulk order

Alternative name: HTDG

Monoclonal antibody raised in mouse against TDG (Thymine-DNA glycosylase), using a recombinant protein.

Lot001
Concentration1 μg/μl
Species reactivityHuman
TypeMonoclonal
PurityAffinity purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 Fig 1
Immunofluorescence 1:400 Fig 2
  • Validation data

    ELISA

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against TDG
    Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against TDG(Cat. No. C15200227) diluted 1:1,000. The position of the TDG and the sumoylated TDG protein is indicated on the right; the marker (in kDa) is shown on the left.

    Dot blot

    Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against TDG
    HeLa cells were stained with the Diagenode antibody against TDG (Cat. No. C15200227) diluted 1:400 followed by an anti-mouse antibody conjugated to Alexa488 (left). The right figure shows staining of the nuclei with DAPI.

  • Target description

    TDG (UniProt/Swiss-Prot entry Q13569) belongs to the TDG/mug DNA glycosylase family. It removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. It is also able to remove thymine from C/T and T/T mispairings, although with lower activity. Furrther, TDG plays a key role in active DNA demethylation as it binds to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), but not 5-hydroxymethylcytosine (5hmC), and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  • Documents
    Datasheet TDG monoclonal antibody - C15200227 DATASHEET
    Monoclonal antibody raised in mouse against TDG (Thymine-DNA glycosylase), using a recombinant pr...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: TDG monoclonal antibody - Classic (Diagenode Cat# C15200227 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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