Diagenode

STAT2 monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200210
100 µg
$325.00
  Bulk order

Alternative names: STAT113, P113, ISGF-3

Monoclonal antibody raised in mouse against STAT2, using a KLH-conjugated synthetic peptide.

Lot001
Concentration1.0 μg/μl
Species reactivityHuman, mouse
TypeMonoclonal
PurityProtein A purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ELISA 1:5,000 - 1:10,000
Western Blotting 1:1,000 Fig 1
Immunohistochemistry 1:2.5 μg/mL Fig 2

* Optimal dilutions/concentrations should be determined by the researcher.

  • Validation Data

    Western Blot

    Figure 1. STAT2 western blot results
    Western blot was performed on 1 μg HeLa whole cell lysate with the STAT2 monoclonal antibody (Cat. No. C15200210). The blot was blocked with 1% BSA in TBST for 30 min at RT, then washed and incubated with the Diagenode STAT2 antibody diluted 1:1,000 in 3% BSA/TBST overnight at 4C.

    immunohistochemistry

    Figure 2. STAT2 antibody immunohistochemistry results
    The Diagenode STAT2 monoclonal antibody was used at a 2.5 μg/mL to detect STAT2 in squamous epithelium from human skin (40X) showing moderate to strong nuclear and faint to moderate cytoplasmic staining (figure 2). Expression of STAT2 is expected to be cytoplasmic, and nuclear upon activation. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IHC
    Immunohistochemistry Read more
  • Documents
    Datasheet STAT2 C15200210 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: STAT2 monoclonal antibody - Classic (Diagenode Cat# C15200210 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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