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Figure 1. STAT2 western blot results Western blot was performed on 1 μg HeLa whole cell lysate with the STAT2 monoclonal antibody (Cat. No. C15200210). The blot was blocked with 1% BSA in TBST for 30 min at RT, then washed and incubated with the Diagenode STAT2 antibody diluted 1:1,000 in 3% BSA/TBST overnight at 4C.
Figure 2. STAT2 antibody immunohistochemistry results The Diagenode STAT2 monoclonal antibody was used at a 2.5 μg/mL to detect STAT2 in squamous epithelium from human skin (40X) showing moderate to strong nuclear and faint to moderate cytoplasmic staining (figure 2). Expression of STAT2 is expected to be cytoplasmic, and nuclear upon activation. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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